13 research outputs found
Peroxidase activity of human superoxide dismutase 1: production of the carbonate radical, covalent dimerization of the enzyme, and implications to amyotrophic lateral sclerosis
A esclerose lateral amiotrófica (ELA) é uma doença neurodegenerativa que afeta os neurônios motores levando a atrofia muscular e morte por insuficiência respiratória. Esta patologia se manifesta de forma esporádica ou familiar, que são indistinguÃveis clinicamente. Mutações na enzima antioxidante superóxido dismutase 1 (hSod1) respondem por aproximadamente 20% dos casos familiares de ELA. Além disso, o caráter autossômico dominante destas mutações revela que a hSod1 adquire propriedades tóxicas aos neurônios motores. Atualmente, duas hipóteses não mutuamente excludentes existem para explicar o caráter tóxico das mutantes da hSod1 relacionadas à ELA. A primeira refere-se à produção de oxidantes pela atividade peroxidásica exacerbada das mutantes contribuindo para o estresse oxidativo observado em ELA. A segunda refere-se à agregação de proteÃnas como ocorre em outras doenças neurodegenerativas. Digno de nota, o radical carbonato produzido na atividade peroxidásica da hSod1 causa a formação de um dÃmero covalente da proteÃna análogo a uma espécie de hSod1 frequentemente detectada em modelos experimentais e pacientes da doença e associada à propriedade tóxica das mutantes. Desta forma, o presente trabalho buscou esclarecer o mecanismo de produção do radical carbonato pela hSod1, bem como caracterizar o dÃmero covalente da proteÃna para posterior estudo de sua formação em um modelo de ELA em ratos que superexpressam a mutante G93A da hSod1. Os estudos cinéticos da variação do pH sobre os efeitos de bicarbonato/CO2, nitrito e formato na atividade peroxidásica da hSod1, medidos pelo consumo de peróxido de hidrogênio e produção de radical, permitiram excluir o mecanismo de Fenton para explicar o ciclo peroxidativo da enzima em tampão bicarbonato em favor de outros intermediários reativos. Já, os experimentos de 13C RMN, modelagem molecular e cinética de fluxo interrompido com mistura assimétrica demonstraram que o ânion peroxomonocarbonato constitui o precursor do radical carbonato produzido pela hSod1. A caracterização do dÃmero covalente da hSod1 por proteólise com tripsina seguida de análise por HPLC/UV-vis e HPLC/ESI-MS identificou um peptÃdeo caracterÃstico do dÃmero covalente da hSod1. A digestão enzimática em H2 18O demonstrou de forma inequÃvoca a natureza dÃmerica deste peptÃdeo pela marcação da extremidade C-terminal. Ainda, o sequenciamento do peptÃdeo dimérico por MS/MS revelou a estrutura primária ESNGPVKVW(ESNGPVKVWGSIK)GSIK, na qual as cadeias polipeptÃdicas estão ligadas através de um aduto de ditriptofano composto por resÃduos Trp32 da proteÃna. Por fim, este peptÃdeo dimérico pode ser empregado como marcador bioquÃmico especÃfico para o estudo do dÃmero covalente da hSod1 in vivo. A análise do extrato de proteÃnas das medulas dos ratos modelo de ELA identificou quinze candidatos a dÃmero covalente da hSod1 por Western-blot, sendo que dois deles foram excluÃdos por espectrometria de massa, pois tiveram o resÃduo Trp32 identificado. O peptÃdeo ESNGPVKVW(ESNGPVKVWGSIK)GSIK não foi observado, porém as treze espécies restantes permanecem candidatas e deverão ser reexaminadas em trabalhos que darão sequência a esta tese de doutorado. Em suma, o peroxomonocarbonato constitui o intermediário na produção do radical carbonato pela hSod1 e o peptÃdeo ESNGPVKVW(ESNGPVKVWGSIK)GSIK uma ferramenta importante no estudo da agregação covalente da hSod1 em ELA.Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease of motors neurons that causes muscle atrophy, weakness, and death by respiratory failure. This pathology occurs in both sporadic and familiar forms that are clinically indistinguishable. Mutations in the antioxidant enzyme superoxide dismutase 1 (hSod1) respond to about 20% of the familiar cases of ALS. Besides, the autosomal dominant nature of these hSod1-associated ALS suggests that the mutants gain toxic properties to motor neurons. Currently, two hypotheses exist to explain the toxicity of hSod1 mutants but they do not exclude each other. The first one is related to the production of oxidants by the increased peroxidase activity of the ALS-linked mutants that could contribute to the oxidative stress reported in ALS. The second refers to protein aggregation as proposed in other neurodegenerative diseases. Noteworthy, the carbonate radical produced during hSod1 peroxidase activity leads to the formation of a covalent dimer of the protein similar to a hSod1 species often detected in experimental models and patients of the disease and implicated in the toxic properties of hSod1 mutants. Thus, the present work aimed to determine the mechanism of carbonate radical production by hSod1 and to characterize the covalent dimer of the protein in vitro followed by the study of covalent aggregates of hSod1 in a rat model of ALS that overexpresses the G93A mutant of the protein. The kinetic studies of the effect of bicarbonate/CO2, nitrite and formate in the peroxidase activity of hSod1 at various pH, measured by hydrogen peroxide consumption and radical production, permitted to exclude the Fenton mechanism to explain the enzyme peroxidative cycle in bicarbonate buffer in favor of other reactive intermediates. Furthermore, 13C NMR, molecular docking and stopped-flow experiments with asymmetric mixing demonstrated that the anion peroxomonocarbonate is the precursor of the carbonate radical produced by hSod1. The characterization of hSod1 covalent dimer by proteolysis with trypsin followed by HPLC/UV-vis and HPLC/ESI-MS analysis identified a peptide characteristic of the covalent dimer of the protein. The enzymatic digestion in H2 18 O irrefutably demonstrated the dimeric nature of this peptide because of the C-terminal labeling with oxygen-18 isotopes. In addition, sequencing of the dimeric peptide by MS/MS determined the primary structure ESNGPVKVW(ESNGPVKVWGSIK)GSIK, in which the polipeptide chains are crosslinked through a ditryptophan adduct formed by a covalent bond between the Trp32 residues of each subunit. So, this dimeric peptide can be employed as a biochemical marker for studying the hSod1 covalent dimer in vivo. The analysis of protein extracts from the spinal cord of the rat model of ALS by Western-blot identified fifteen candidates to hSod1 covalent dimer, but two of them were excluded by mass spectrometry analysis that identified unmodified Trp32 residues. Moreover, neither the dimeric peptide nor the Trp32 residue were observed in the remaining species. Therefore, these thirteen candidates must be reexamined in subsequent studies. In conclusion, the anion peroxomonocarbonate is the key intermediate in the production of the carbonate radical by hSod1 and the dimeric peptide constitutes a specific tool to study hSod1 covalent aggregation in AL
Proteostasis disturbance in amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease affecting motoneurons in the brain and spinal cord leading to paralysis and death. Although the etiology of ALS remains poorly understood, abnormal protein aggregation and altered proteostasis are common features of sporadic and familial ALS forms. The proteostasis network is decomposed into different modules highly conserved across species and comprehends a collection of mechanisms related to protein synthesis, folding, trafficking, secretion and degradation that is distributed in different compartments inside the cell. Functional studies in various ALS models are revealing a complex scenario where distinct and even opposite effects in disease progression are observed depending on the targeted component of the proteostasis network. Importantly, alteration of the folding capacity of the endoplasmic reticulum (ER) is becoming a common pathological alteration in ALS, representing one of the earliest defects observed in disease models, contributing to denervation and motoneuron dysfunction. Strategies to target-specific components of the proteostasis network using small molecules and gene therapy are under development, and promise interesting avenues for future interventions to delay or stop ALS progressionFONDAP program
15150012
US Office of Naval Research-Global (ONR-G)
N62909-16-1-2003
Millennium Institute
P09-015-F
FONDEF
ID16I10223
D11E1007
US Air Force Office of Scientific Research
FA9550-16-1-0384
CONICYT-Brazil
441921/2016-7
ALS Therapy Alliance
2014-F-059
Muscular Dystrophy Association
382453
Michael J Fox Foundation for Parkinsons Research - Target Validation grant
9277
FONDECYT
1140549
11150579
3170622
Office of the Assistant Secretary of Defense for Health Affairs through the ALSRP Therapeutic Idea Award
W81XWH-16-1-0112
ALS Association
17-PDF-36
Fine-tuning ER stress signal transducers to treat amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease characterized by the progressive loss of motoneurons and paralysis. The mechanisms underlying neuronal degeneration in ALS are starting to be elucidated, highlighting disturbances in motoneuron proteostasis. Endoplasmic reticulum (ER) stress has emerged as an early pathogenic event underlying motoneuron vulnerability and denervation in ALS. Maintenance of ER proteostasis is controlled by a dynamic signaling network known as the unfolded protein response (UPR). Inositol-requiring enzyme 1 (IRE1) is an ER-located kinase and endoribonuclease that operates as a major ER stress transducer, mediating the establishment of adaptive and pro-apoptotic programs. Here we discuss current evidence supporting the role of ER stress in motoneuron demise in ALS and build the rational to target IRE1 to ameliorate neurodegeneration.Congressionally Directed Medical Research Programs / Muscular Dystrophy Association, 382453 /
FONDAP program, 15150012 / Millennium Institute, P09-015-F /
Fondo Nacional de Desarrollo Cientifico y Tecnologico (FONDECYT), 11150579 /
Michael J. Fox Foundation for Parkinson's Research-Target Validation grant, 9277 /
FONDEF, ID16I10223, D11E1007 / FONDECYT, 1140549 /
US Office of Naval Research-Global (ONR-G), N62909-16-1-2003 /
US Air Force Office of Scientific Research, FA9550-16-1-0384 /
European Commission RD, MSCA-RISE, 734749 / CONICYT-Brazil, 441921/2016-7 /
ALSRP Therapeutic Idea Award, AL15011
Creutzfeldt-jakob disease. Report of one case DesafÃos en el diagnóstico de enfermedad de Creutzfeldt-Jakob. Caso clÃnico
© 2016, Sociedad Medica de Santiago. All rights reserved. Creutzfeldt-Jakob disease has a higher incidence in Chile than in other countries. The post mortem pathological characterization of brain tissue is necessary to reach a definitive diagnosis. We report a 73 years old man with a history compatible with of a rapidly progressive dementia, in which the first electroencephalographic study showed a pattern consistent with non-convulsive status epilepticus. Besides discarding this diagnosis, it was necessary to rule out other causes of rapidly progressive dementia such as Hashimoto encephalopathy. Finally, the sustained clinical deterioration with no response to anticonvulsants and corticosteroids, the imaging studies, a serial electroencephalographic monitoring study and the detection of 14-3-3 protein in cerebrospinal fluid were the keys to achieve the diagnosis of the disease
Oligomerization of Cu,Zn-Superoxide Dismutase (SOD1) by Docosahexaenoic Acid and Its Hydroperoxides In Vitro: Aggregation Dependence on Fatty Acid Unsaturation and Thiols.
Docosahexaenoic acid (C22:6, n-3, DHA) is a polyunsaturated fatty acid highly enriched in the brain. This fatty acid can be easily oxidized yielding hydroperoxides as primary products. Cu, Zn-Superoxide dismutase (SOD1) aggregation is a common hallmark of Amyotrophic Lateral Sclerosis (ALS) and the molecular mechanisms behind their formation are not completely understood. Here we investigated the effect of DHA and its hydroperoxides (DHAOOH) on human SOD1 oligomerization in vitro. DHA induced the formation of high-molecular-weight (HMW) SOD1 species (>700 kDa). Aggregation was dependent on free thiols and occurred primarily with the protein in its apo-form. SOD1 incubation with DHA was accompanied by changes in protein structure leading to exposure of protein hydrophobic patches and formation of non-amyloid aggregates. Site-directed mutagenesis studies demonstrated that Cys 6 and Cys 111 in wild-type and Cys 6 in ALS-linked G93A mutant are required for aggregation. In contrast, DHAOOH did not induce HMW species formation but promoted abnormal covalent dimerization of apo-SOD1 that was resistant to SDS and thiol reductants. Overall, our data demonstrate that DHA and DHAOOH induce distinct types of apo-SOD1 oligomerization leading to the formation of HMW and low-molecular-weight species, respectively
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Functional Role of the Disulfide Isomerase ERp57 in Axonal Regeneration
ArtÃculo de publicación ISIERp57 (also known as grp58 and PDIA3) is a protein disulfide isomerase that catalyzes
disulfide bonds formation of glycoproteins as part of the calnexin and calreticulin cycle.
ERp57 is markedly upregulated in most common neurodegenerative diseases downstream
of the endoplasmic reticulum (ER) stress response. Despite accumulating correlative evidence
supporting a neuroprotective role of ERp57, the contribution of this foldase to the
physiology of the nervous system remains unknown. Here we developed a transgenic
mouse model that overexpresses ERp57 in the nervous system under the control of the
prion promoter. We analyzed the susceptibility of ERp57 transgenic mice to undergo neurodegeneration.
Unexpectedly, ERp57 overexpression did not affect dopaminergic neuron
loss and striatal denervation after injection of a Parkinson’s disease-inducing neurotoxin. In
sharp contrast, ERp57 transgenic animals presented enhanced locomotor recovery after
mechanical injury to the sciatic nerve. These protective effects were associated with
enhanced myelin removal, macrophage infiltration and axonal regeneration. Our results
suggest that ERp57 specifically contributes to peripheral nerve regeneration, whereas its
activity is dispensable for the survival of a specific neuronal population of the central nervous
system. These results demonstrate for the first time a functional role of a component of
the ER proteostasis network in peripheral nerve regeneration.FONDEF
D11I1007
Millennium Institute
P09-015-F
Frick Foundation
FONDECYT
1140549
Michael J Fox Foundation for Parkinson Research
Alzheimer's Association
Muscular Dystrophy Association
COPEC-UC Foundation
CONICYT
USA2013-0003
ECOS-CONICYT
C13S02
ALS Therapy Alliance
Millennium Nucleus
P-07-011-F
CONICYT PAI
82130031
ACT110
Nature of the apo-SOD1 aggregates formed in the presence of DHA or DHAOOH.
<p>Representative bis-ANS fluorescence spectra obtained for the control incubations containing DHA, DHAOOH or H<sub>2</sub>O<sub>2</sub> without the protein (A); and for the incubations containing the (B) apo-SOD1 WT or (C) apo-SOD1 G93A incubated in the presence of DHA, DHAOOH or H<sub>2</sub>O<sub>2</sub>. Transmission electronic microscopy (TEM) of the apo-SOD1 WT or G93A mutant incubated with DHA (D). Two sets of images, one with the scale bar of 500 nm (upper panel) and the other of 100 nm (lower panel) are shown. Images are representative of two experiments. Representative visible spectra of congo red (CR) in the absence and presence of apo-SOD1 WT or G93A mutant pre-incubated with DHA or DHAOOH. Samples were added to the CR solution to give a final concentration of 6 μM of CR. All incubations were conducted in the presence of 10 μM protein and 250 μM of the lipid or H<sub>2</sub>O<sub>2</sub> at 37°C for 24 h.</p
Role of Cys 6 and Cys 111 on DHA induced apo-SOD1 aggregation.
<p>C6S and C111S mutants of apo-SOD1 (10 μM) WT (<b>A</b>) and G93A (<b>B</b>) were incubated in the absence and presence of DHA (250 μM). Percentages of aggregates formed in the incubation were determined by size-exclusion chromatography analysis. The results were presented by means ± standard deviations of three experiments. Significant differences are indicated with * when <i>p<0</i>.<i>01</i>. Dots represent individual values.</p
Analysis of apo-SOD1 aggregates by size-exclusion chromatography.
<p>Apo-SOD1 WT incubated in the absence (<b>A</b>) and presence of 250 μM of DHA (<b>B</b>), DHAOOH <b>(C)</b> or H<sub>2</sub>O<sub>2</sub><b>(D)</b>. Apo-SOD1 G93A incubated in the absence <b>(E)</b> and presence of 250 μM of DHA <b>(F)</b>, DHAOOH <b>(G)</b>, or H<sub>2</sub>O<sub>2</sub><b>(H)</b>. All experiments were conducted in the presence of 10 μM of the protein at 37°C for 24 h. The colored lines represent the incubation times: 2 h (black), 6 h (red) and 24 h (blue). Chromatograms are representative at least 3 independent experiments.</p