170 research outputs found
Modulating endothelial adhesion and migration impacts stem cell therapies efficacy
Background: Limited knowledge of stem cell therapies‘ mechanisms of action hampers their sustainable implementation into the clinic. Specifically, the interactions of transplanted stem cells with the host vasculature and its implications for their therapeutic efficacy are not elucidated. We tested whether adhesion receptors and chemokine receptors on stem cells can be functionally modulated, and consequently if such modulation may substantially affect therapeutically relevant stem cell interactions with the host endothelium. Methods: We investigated the effects of cationic molecule polyethylenimine (PEI) treatment with or without nanoparticles on the functions of adhesion receptors and chemokine receptors of human bone marrow-derived Mesenchymal Stem Cells (MSC). Analyses included MSC functions in vitro, as well as homing and therapeutic efficacy in rodent models of central nervous system´s pathologies in vivo. Findings: PEI treatment did not affect viability, immunomodulation or differentiation potential of MSC, but increased the CCR4 expression and functionally blocked their adhesion receptors, thus decreasing their adhesion capacity in vitro. Intravenously applied in a rat model of brain injury, the homing rate of PEI-MSC in the brain was highly increased with decreased numbers of adherent PEI-MSC in the lung vasculature. Moreover, in comparison to untreated MSC, PEI-MSC featured increased tumour directed migration in a mouse glioblastoma model, and superior therapeutic efficacy in a murine model of stroke. Interpretation: Balanced stem cell adhesion and migration in different parts of the vasculature and tissues together with the local microenvironment impacts their therapeutic efficacy. Funding: Robert Bosch Stiftung, IZEPHA grant, EU grant 7 FP Healt
Discovery of Very High Energy gamma-rays from 1ES 1011+496 at z=0.212
We report on the discovery of Very High Energy (VHE) gamma-ray emission from
the BL Lacertae object 1ES1011+496. The observation was triggered by an optical
outburst in March 2007 and the source was observed with the MAGIC telescope
from March to May 2007. Observing for 18.7 hr we find an excess of 6.2 sigma
with an integrated flux above 200 GeV of (1.58 photons
cm s. The VHE gamma-ray flux is >40% higher than in March-April
2006 (reported elsewhere), indicating that the VHE emission state may be
related to the optical emission state. We have also determined the redshift of
1ES1011+496 based on an optical spectrum that reveals the absorption lines of
the host galaxy. The redshift of z=0.212 makes 1ES1011+496 the most distant
source observed to emit VHE gamma-rays up to date.Comment: 4 pages, 6 figures, minor changes to fit the ApJ versio
MAGIC observations of very high energy gamma-rays from HESS J1813-178
Recently, the HESS collaboration has reported the detection of gamma-ray
emission above a few hundred GeV from eight new sources located close to the
Galactic Plane. The source HESS J1813-178 has sparked particular interest, as
subsequent radio observations imply an association with SNR G12.82-0.02.
Triggered by the detection in VHE gamma-rays, a positionally coincident source
has also been found in INTEGRAL and ASCA data. In this Letter we present MAGIC
observations of HESS J1813-178, resulting in the detection of a differential
gamma-ray flux consistent with a hard-slope power law, described as dN/(dA dt
dE) = (3.3+/-0.5)*10^{-12} (E/TeV)^{-2.1+/-0.2} cm^(-2)s^(-1)TeV^(-1). We
briefly discuss the observational technique used, the procedure implemented for
the data analysis, and put this detection in the perspective of multifrequency
observations.Comment: Accepted by ApJ Letter
Systematic search for VHE gamma-ray emission from X-ray bright high-frequency BL Lac objects
All but three (M87, BL Lac and 3C 279) extragalactic sources detected so far
at very high energy (VHE) gamma-rays belong to the class of high-frequency
peaked BL Lac (HBL) objects. This suggested to us a systematic scan of
candidate sources with the MAGIC telescope, based on the compilation of X-ray
blazars by Donato et al. (2001). The observations took place from December 2004
to March 2006 and cover sources on the northern sky visible under small zenith
distances zd < 30 degrees at culmination. The sensitivity of the search was
planned for detecting X-ray bright F(1 keV) > 2 uJy) sources emitting at least
the same energy flux at 200 GeV as at 1 keV. In order to avoid strong gamma-ray
attenuation close to the energy threshold, the redshift of the sources was
constrained to values z<0.3. Of the fourteen sources observed, 1ES 1218+304 and
1ES 2344+514 have been detected in addition to the known bright TeV blazars Mrk
421 and Mrk 501. A marginal excess of 3.5 sigma from the position of 1ES
1011+496 was observed and has been confirmed as a source of VHE gamma-rays by a
second MAGIC observation triggered by a high optical state (Albert et al.
2007). For the remaining sources, we present here the 99% confidence level
upper limits on the integral flux above ~200 GeV. We characterize the sample of
HBLs (including all HBLs detected at VHE so far) by looking for correlations
between their multi-frequency spectral indices determined from simultaneous
optical, archival X-ray, and radio luminosities, finding that the VHE emitting
HBLs do not seem to constitute a unique subclass. The absorption corrected
gamma-ray luminosities at 200 GeV of the HBLs are generally not higher than
their X-ray luminosities at 1 keV.Comment: 15 pages, 7 figures, 5 tables, submitted to ApJ (revised version
Constraints on the steady and pulsed very high energy gamma-ray emission from observations of PSR B1951+32/CTB 80 with the MAGIC Telescope
We report on very high energy gamma-observations with the MAGIC Telescope of
the pulsar PSR B1951+32 and its associated nebula, CTB 80. Our data constrain
the cutoff energy of the pulsar to be less than 32 GeV, assuming the pulsed
gamma-ray emission to be exponentially cut off. The upper limit on the flux of
pulsed gamma-ray emission above 75 GeV is 4.3*10^-11 photons cm^-2 sec^-1, and
the upper limit on the flux of steady emission above 140 GeV is 1.5*10^-11
photons cm^-2 sec^-1. We discuss our results in the framework of recent model
predictions and other studies.Comment: 7 pages, 7 figures, replaced with published versio
First bounds on the very high energy gamma-ray emission from Arp 220
Using the Major Atmospheric Gamma Imaging Cherenkov Telescope (MAGIC), we
have observed the nearest ultra-luminous infrared galaxy Arp 220 for about 15
hours. No significant signal was detected within the dedicated amount of
observation time. The first upper limits to the very high energy -ray
flux of Arp 220 are herein reported and compared with theoretical expectations.Comment: Accepted for publication in Ap
Upper limit for gamma-ray emission above 140 GeV from the dwarf spheroidal galaxy Draco
The nearby dwarf spheroidal galaxy Draco with its high mass to light ratio is
one of the most auspicious targets for indirect dark matter searches.
Annihilation of hypothetical DM particles can result in high-energy gamma-rays,
e.g. from neutralino annihilation in the supersymmetric framework. With the
MAGIC telescope a search for a possible DM signal originating from Draco was
performed during 2007. The analysis of the data results in a flux upper limit
of 1.1x10^-11 photons cm^-2 sec^-1 for photon energies above 140 GeV, assuming
a point like source. Furthermore, a comparison with predictions from
supersymmetric models is given. While our results do not constrain the mSUGRA
phase parameter space, a very high flux enhancement can be ruled out.Comment: Accepted for publication by Astrophysical Journa
Implementation of the Random Forest Method for the Imaging Atmospheric Cherenkov Telescope MAGIC
The paper describes an application of the tree classification method Random
Forest (RF), as used in the analysis of data from the ground-based gamma
telescope MAGIC. In such telescopes, cosmic gamma-rays are observed and have to
be discriminated against a dominating background of hadronic cosmic-ray
particles. We describe the application of RF for this gamma/hadron separation.
The RF method often shows superior performance in comparison with traditional
semi-empirical techniques. Critical issues of the method and its implementation
are discussed. An application of the RF method for estimation of a continuous
parameter from related variables, rather than discrete classes, is also
discussed.Comment: 16 pages, 8 figure
Unfolding of differential energy spectra in the MAGIC experiment
The paper describes the different methods, used in the MAGIC experiment, to
unfold experimental energy distributions of cosmic ray particles (gamma-rays).
Questions and problems related to the unfolding are discussed. Various
procedures are proposed which can help to make the unfolding robust and
reliable. The different methods and procedures are implemented in the MAGIC
software and are used in most of the analyses.Comment: Submitted to NIM
Discovery of Very High Energy -Rays from Markarian~180 Triggered by an Optical Outburst
The high-frequency-peaked BL Lacertae object Markarian~180 (Mrk~180) was
observed to have an optical outburst in 2006 March, triggering a Target of
Opportunity observation with the MAGIC telescope. The source was observed for
12.4 hr and very high energy -ray emission was detected with a
significance of 5.5 . An integral flux above 200 GeV of
was measured, corresponding to
11% of the Crab Nebula flux. A rather soft spectrum with a photon index of
has been determined. No significant flux variation was found.Comment: Accepted by ApJ Letters, minor revision
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