Biosynthesis and characterization of silver nanoparticles produced with aqueous extract of Pterodon emarginatus Vogel - Fabaceae seeds associated with gentamicin sulfate and hyaluronic acid with potential antimicrobial activity / Biossíntese e caracterização de nanopartículas de prata produzidas com extrato aquoso de sementes de Pterodon emarginatus Vogel - Fabaceae associadas ao sulfato de gentamicina e ao Ácido Hialurônico com potencial atividade antimicrobiana

The synthesis of metallic nanoparticles by biological systems is known as green synthesis or biosynthesis. In this synthesis strategy, instead of using toxic agents to reduce the metal ion, obtaining the metallic nanoparticles (NPs) is mediated by biological components capable of reducing the metal. In this context, the present work uses aqueous extract from the seeds of Pterodon emarginatus Vogel to obtain silver nanoparticles (AgNPs-PE). These metallic nanoparticles associated with the aqueous extract of P. emarginatus have, among others, antimicrobial property that was enhanced by the association of 1% gentamicin sulfate (AgNPs-PEG) and hyaluronic acid (AgNPs-PEG-AH2). Therefore, the evaluation of antimicrobial activity was addressed after obtaining the AgNPs-PE and their associations. The characterization of AgNPs-PE was performed by means of UV-Vis spectrophotometry, determination of the average hydrodynamic diameter (AHD), zeta potential (ZP) and polydispersity index (PDI), field emission gun scanning electron microscopy (FEG-SEM), energy dispersive X-ray spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopy. Although the green synthesis still does not have its mechanisms clearly elucidated, it is known that the bioreduction of Ag+ and the stabilization of Ag0 are carried out by a combination of primary and secondary metabolites present in the extract, such as tannins and flavonoids. After formed, AgNPs-PE, AgNPs-PEG and AgNPs-PEG-AH2 showed, respectively, maximum absorbance around 423 nm, 425 nm and 430 nm. Microscopy (FEG-SEM) showed spherical-shaped nanoparticles, with an average size ranging from 59 nm for AgNPs-PE, 63 nm for AgNPs-PEG and 66 nm for AgNPs-PEG-AH2. The ZP of all AgNPs-PE and their associations remained within the values considered stable for nanoparticles (-27.15 to -39.73 mV). The PDI of the analyzed samples was on average 0.332 to 0.524, median values for the distribution of NPs. AgNPs-PE, AgNPs-PEG and AgNPs-PEG-AH2 showed bactericidal activity against Gram-positive, Gram-negative and yeast bacteria. The present research indicates that AgNPs-PE and their associations have potential as a therapeutic agent against bacterial and fungal infections

PREVALÊNCIA DE DANO HEPÁTICO EM EX-ALCOOLISTAS DE UM GRUPO DE ALCOÓLICOS ANÔNIMOS DE PONTA GROSSA – PR

O álcool vem sendo apontado como a droga mais consumida no Brasil e o seu consumo em excesso pode gerar danos hepáticos. Pessoas que fazem uso abusivo, depois de alguns anos, podem sofrer com essas complicações. O objetivo deste trabalho foi avaliar os parâmetros bioquímicos das enzimas Transaminase Glutâmica Oxalacética e Pirúvica (TGO e TGP), Gama Glutamil Transferase (GAMAGT) e o doseamento dos níveis séricos de Albumina de 30 pacientes de um grupo de Alcóolicos Anônimos de Ponta Grossa – Pr. Todos os pacientes avaliados eram do sexo masculino, com uma prevalência maior da faixa etária de 30 – 50 anos. Com relação as enzimas hepáticas deste grupo, o TGO teve uma média entre os pacientes de 28,8 U/L, o TGP teve uma média de 32,1 U/L, já o GAMA-GT teve uma média de 40,8 U/L. Para os níveis séricos de Albumina obteve-se uma média de e 4,9 mg/dL. Assim, os parâmetros bioquímicos em geral estavam dentro dos valores de referência mas as dosagens de TGO e GAMA-GT apresentaram diferença estatística quando comparados ao grupo controle

Chemical Composition and Biological Activities of The Essential Oil And Anatomical Markers Of Lavandula Dentata L. Cultivated In Brazil

ABSTRACT Lavandula dentata, popularly known as lavender, is commonly used in traditional medicine for the treatment of digestive and inflammatory disorders. The objective of this study was to analyzed the chemical oil composition, antioxidant and antimicrobial activities of the essential oil and anatomical markers of the leaf and stem of L. dentata cultivated in South Brazil. Essential oil showed an antioxidant activity similar to rutin and gallic acid when analyzed by phosphomolybdenum method. However, by the free radical DPPH and ABTS methods, it showed a slight potential antioxidant. Essential oil presented 1,8-cineol (63%) as major component, antimicrobial activity against Gram-positive, Gram-negative bacteria strains and Candida albicans, by broth microdilution. The anatomical profile provided the following main microscopic markers: hypostomatic leaves; diacytic stomata, thin and striate cuticle; multicellular and branched non-glandular trichomes; capitate glandular trichomes; peltate glandular trichomes; dorsiventral mesophyll; flat-convex shape midrib, truncated on the abaxial side; one collateral vascular bundle in the midrib; square stem shape, angular collenchyma alternated with cortical parenchyma; sclerenchymatic fibers well-developed on the four edges