Biocompatibility of acetazolamide pastes in the subcutaneous tissue of rats

This aim of this study was to investigate the biocompatibility of two experimental acetazolamide (AZ)-based pastes in the subcutaneous tissue of rats. Both pastes contained AZ as the main component in similar concentration. The vehicle in experimental paste 1 was saline, while experimental paste 2 was prepared with propylene glycol. Sixty polyethylene tubes were sealed at one end with gutta-percha (GP), which served as a control. Half of the tubes were filled with paste 1 and half with paste 2. The tubes were implanted in the subcutaneous tissue of 15 rats, being 4 tubes for each animal. The animals were killed 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin and eosin and were analyzed by light microscopy. Scores were assigned to level of inflammatory process: 1- none; 2- mild; 3- moderate; 4- severe. The data were analyzed statistically by the Kruskal-Wallis test (p&#8804;0.05). Paste 1 produced an inflammatory process at 7 days. However, the intensity of this inflammation decreased with time and was nearly absent at 45 days. No statistically significant difference (p>0.05) was observed between the control (GP) and paste 1. However, paste 2 produced inflammatory response at all study periods and differed significantly (p<0.05) from the control. In conclusion, in the present study, the experimental AZ-based paste 1 was considered as biocompatible as the control matrial (GP), while experimental paste 2 was irritating to rat subcutaneous tissue.Este estudo investigou a biocompatibilidade de pastas experimentais a base de acetazolamida em tecido subcutâneo de rato. Duas pastas foram usadas neste estudo. Ambas continham a acetalozamida como componente principal em concentrações similares. O veículo usado na pasta experimental 1 foi o soro fisiológico e na pasta experimental 2 foi o propilenoglicol. Sessenta tubos de polietileno foram selados em uma das extremidades com guta-percha, que serviu como controle. Metade dos tubos foi preenchida com a pasta 1 e metade com a pasta 2. Os tubos foram introduzidos no tecido subcutâneo de 15 ratos (4 tubos por animal). Aos 7, 15 e 45 dias após a cirurgia, os animais foram sacrificados e os espécimes processados em laboratório. Os cortes histológicos foram corados com hematoxilina e eosina e analisados em microscópio de luz. Escores foram estabelecidos de acordo com a intensidade do processo inflamatório: 1-sem inflamação; 2-discreta; 3-moderada; 4-severa. Os dados obtidos foram comparados estatisticamente através do teste de Kruskal-Wallis (p&#8804;0,05). A pasta 1 promoveu processo inflamatório aos 7 dias. Entretanto, sua intensidade diminuiu com o tempo e estava praticamente ausente aos 45 dias. Não foi observada diferença estatisticamente significante entre o controle (guta-percha) e a pasta 1. Entretanto, a pasta 2 promoveu reação inflamatória em todos os períodos experimentais, com diferença estatisticamente significante em relação ao controle. Assim, a pasta experimental de acetazolamida 1 foi considerada biocompatível como o controle deste trabalho. Já a pasta experimental 2 foi irritante aos tecidos

Avulsion of permanent teeth: analysis of the efficacy of na informative campaign for professionals from elementary schools

Tooth avulsion is a very common event in children and emergency care procedures are crucial for case prognosis. As anyone at the moment of accident can provide first-aid measures, knowledge of school professionals dealing with children is of paramount importance. An informative campaign about dental trauma was performed in urban schools in the city of Adamantina, São Paulo state, Brazil, in an attempt to educate school professionals about emergency procedures in cases of tooth avulsion. A questionnaire arguing on personal information, tooth avulsion knowledge and personal impressions of the campaign was handed out for evaluation of the informative campaign. Eighty school professionals participated in the survey. Most participants were females (93.75%) aged 41-60 years old (53.75%) with a college degree (77.5%). Time at work ranged from 1-5 years (22.5%). The efficacy of the campaign was considered positive, as 100% of the interviewees replied to the importance of emergency procedures in case of tooth avulsion. In addition, 81.25% of the respondents answered that they would store the avulsed tooth in some storage medium, of which milk was the most frequently mentioned (84.61%). The participants considered the campaign as satisfactory and a region-wide campaign will be accomplished at other schools

Forças em estruturas de acostagem descontínuas em zonas desabrigadas: avaliação de ferramentas de cálculo existentes

Dissertação para obtenção do grau de Mestre em Engenharia Civil na Área de especialização em HidráulicaO crescimento dos navios e do seu respetivo calado tem levado a uma necessidade cada vez maior de estabelecer postos de acostagem em zonas de maior profundidade e muitas vezes fora das bacias portuárias, onde a agitação marítima é mais favorável. Nesse âmbito, surgem os jetties, um tipo de estrutura de acostagem, composta, na sua forma mais geral, por uma plataforma, elevada sobre o nível do mar, com o auxílio de estacas e sustentada por vigas, nas quais os navios acostam e descarregam mercadorias. No entanto, estas estruturas desprotegidas estão sujeitas a forças de difícil quantificação que resultam do cariz aleatório do mar e que introduzem um problema de difícil resolução, tanto para engenheiros, como para investigadores nesta área específica de conhecimento. Nesse âmbito, nesta dissertação são abordados para além dos tipos de estruturas de acostagem, com um enfoque especial em jetties, as forças a que estas estão sujeitas, também designadas de wave-in-deck loadse algumas das diferentes ferramentas de cálculo existentes para a sua quantificação. As ferramentas de cálculo consideradas dividem-se em empíricas hidrostáticas (McConnell et al. (2004), Douglass et al. (2006) e Cuomo et al. (2007)) e semiempíricas cinemáticas (Kaplan et al. (1995) e API (2002)), num total de 5. É realizada uma análise de sensibilidade das ferramentas de cálculo para diferentes condições de agitação, para diferentes níveis de maré e de profundidade, numa plataforma de 20 m de comprimento, 0.16 m de espessura e 5 m de largura, sustentada por 6 vigas, igualmente espaçadas, transversais ao sentido de propagação das ondas, de 5 m de comprimento, 0.2 m de largura e 0.5 m de altura. Por fim, são estabelecidas análises individuais e comparados os diferentes métodos considerados, estabelecidas conclusões para as condições de agitação definidas previamente e propostas sugestões para desenvolvimentos futuros na temática em estudo.Abstract: The increase of ship’s dimensions, including their draft, has led to an increasing need for berths in deeper areas and often out of port basins, where sea waves are more favorable. It is in this context that jetties are used. They are a kind of docking structures, consisting, in its most general form, of a platform, raised above sea level, with the aid of piles and supported by beams, where ships berth to and unload goods. However, the design of these unprotected structures is difficult mainly due to the random nature of sea waves that introduce a difficult problem to solve by both engineers and researchers in this specific area of knowledge. In this context, this thesis will address the different types of berthing structures, with a special emphasis on jetties, forces that those structures are subject to, also known as wave-in-deck loads, and the existing calculation tools for their quantification. The considered calculation tools are divided into hydrostatic empirical (McConnell et al. (2004) Douglass et al. (2006) Cuomo et al. (2007)) and kinematic semi-empirical (Kaplan et al. (1995) and API (2002)), in a total of 5. A sensitivity analysis, of the calculation tools, for different sea state conditions, different tide and depth levels, in a 20 m long, 0.16 m thick and 5 m wide six beam supported platform, is made in which the beams are transverse to the wave propagation direction, 5 m long, 0.2 m wide and 0.5 m high. Finally, the considered methods are analyzed both individually and between themselves, conclusions according to the previously established sea state conditions are taken and future development proposals are made in the subject under study

Effect of zoledronic acid used in the root surface treatment of late replanted teeth: A study in rats

This study evaluated the use of zoledronic acid, a resorption inhibitor, as a medication for root resorption treatment of late replanted teeth. Twenty-four maxillary right central incisors of rats were avulsed and kept dry for 30 min. Then, the teeth were divided into 2 groups. In group I, root surface was treated with 2% sodium fluoride for 20 min; in group II, 10-6M zoledronic acid solution was used for 20 min. All root canals were filled with calcium hydroxide. Next, teeth were replanted in their respective sockets. After 15 and 60 days post-replantation, the animals were killed and the anatomic pieces were obtained and prepared for microscopic and morphometric analyses. The results showed that zoledronic acid was capable of limiting the occurrence of root resorption and preserving cementum resorption. Further research must be performed to confirm the use of zoledronic acid in root surface treatment of late replanted teeth

Propolis as storage media for avulsed teeth: microscopic and morphometric analysis in rats

The maintenance of the avulsed teeth in appropriate media for preserving the cellular viability has been important for repairing the periodontal ligament and preventing the root resorption after tooth reimplantation. Propolis is a substance capable of preserving cellular viability. This study aimed to analyze the propolis substance as a storage media for maintaining the avulsed teeth, besides to determine the ideal time period for keeping the tooth inside it. Thus, 60 maxillary right central incisors of rats were extracted and divided into five groups. In groups I and II, teeth were kept in propolis for 60 min and 6 h, respectively; in group III, teeth were kept in milk for 6 h; in group IV, teeth were kept dry for 60 min; and in group V, they were immediately reimplanted. All teeth had their root canals filled with calcium hydroxide paste. Following, teeth were reimplanted in their sockets. After 15 and 60 days, animals were killed and the obtained samples were processed in laboratory for microscopic and morphometric analyzing. The results showed that the occurrence of inflammatory resorption, dental ankylosis and the formation of the connective tissue parallel to the root surface were similar among groups. It could be verified a greater occurrence of replacement resorption in group IV when comparing to other groups. In groups I and IV, the presence of periodontal ligament-like connective tissue was substantially smaller than the other groups. Regarding to the cementum amount over the root, it could be observed that this was present in smaller amount in groups I and IV. Group II was similar to groups III and IV. Therefore, according to the results of this study, the use of propolis as a storage media for maintaining avulsed teeth could be highlighted, and the 6-h period was more appropriate than the 60-min period

E-Logistics. Alter Wein in neuen Schläuchen?

Alendronate is a known inhibitor of root resorption and the development of alendronate paste would enhance its utilization as intracanal medication. Therefore, this study aimed to investigate the biocompatibility of experimental alendronate paste in subcutaneous tissue of rats, for utilization in teeth susceptible to root resorption. The study was conducted on 15 male rats, weighing similar to 180-200 grams. The rats` dorsal regions were submitted to one incision on the median region and, laterally to the incision, the subcutaneous tissue was raised and gently dissected for introduction of two tubes, in each rat. The tubes were sealed at one end with gutta-percha and taken as control. The tubes were filled with experimental alendronate paste. The animals were killed at 7, 15 and 45 days after surgery and the specimens were processed in laboratory. The histological sections were stained with hematoxylin-eosin and analyzed by light microscopy. Scores were assigned to the in. ammatory process and statistically compared by the Tukey test (P < 0.05). Alendronate paste promoted severe inflammation process at 7 days, with statistically significant difference compared to the control (P < 0.05%). However, at 15 days, there was a regression of in. ammation and the presence of connective tissue with collagen fibers, fibroblasts and blood vessels was observed. After 45 days, it was observed the presence of well-organized connective tissue, with collagen fibers and fibroblasts, and few in. ammatory cells. No statistical difference was observed between the control and experimental paste at 15 and 45 days. The experimental alendronate paste was considered biocompatible with subcutaneous tissue of rat