Observation of intraseasonal oscillation of 64 day in the ionospheric sporadic E layers using Indian MST Radar, SABER / TIMED instrument and Ionosonde present at Gadanki(13.5 N,79.2 E)

15th MST Radar WorkshopSession M6: Middle atmosphere dynamics and structureMay 30 (Tue), Poster Sessio

Precisely Alternating Functionalized Polyampholytes Prepared in a Single Pot from Sustainable Thiolactone Building Blocks

Polyampholytes with precisely alternating cationic and anionic functional groups were prepared using sustainable thiolactone building blocks in a simple one-pot procedure at room temperature and in water. Ring opening of the <i>N-</i>maleamic acid-functionalized homocysteine thiolactone monomer enabled the introduction of different functional groups into the polymer chain, which contributed to both ionic and hydrogen bonding interactions. The resulting polyampholytes exhibited various isoelectric points while maintaining high solubility in water under different pH and ionic strengths, which expands their potential applications. Finally, it is shown that the upper critical solution temperature (UCST) of these alternating polyampholytes in water/ethanol (30/70% vol) solutions can be tuned as a function of the content of ionic and hydroxyl groups

Additional file 10: Figure S5. of Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine

Diet-based comparisons of enzyme expression in pantothenate pathway. Two comparisons are shown: (A) Plin2-HF vs. Plin2-LF and (B) WT-HF vs. WT-LF. Circular nodes indicate enzymes, with size indicating relative difference in expression between sample types and color indicating direction of change (see inset key). Associated heatmaps indicate global changes in expression for each enzyme, in addition to taxon-specific changes in expression for each of the 17 defined taxa colored according to phylum. The following abbreviations are used: 5,6-dh-uracil (5,6-dihydro-uracil), N-cm-β-alanine (N-carbamoyl-β-alanine), N-pt-Cys (N-pantothenoyl-cysteine), and (R)-4′-P-pt-L-Cys ((R)-4′-phospho-pantothenoyl-l-cysteine. (PDF 1119 kb

Additional file 3: Figure S1. of Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine

Principal component analysis for four data types (Taxa, Enzymes, Significant Differentially Expressed Transcripts and Metabolic Pathways). With each plot, p values (< 0.05) are provided indicating significant differences in clustering between each of the four pairwise comparisons. (PDF 912 kb

Additional file 8: Figure S3. of Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine

Comparison of glycolysis pathway enzyme expression between sample types. Three comparisons are shown: (A) Plin2-LF vs. WT-LF; (B) Plin2-HF vs. Plin2-LF; (C) WT-HF vs. WT-LF. Circular nodes indicate enzymes, with size indicating relative difference in expression between sample types and color indicating direction of change (see inset key). Associated heatmaps indicate global changes in expression for each enzyme, in addition to taxon-specific changes in expression for each of the 17 defined taxa colored according to phylum. (PDF 1138 kb

Additional file 2: Table S2. of Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine

Expression values (RPKM) for each of the 57,736 transcripts identified across all samples. (XLSX 35991 kb

Additional file 14: Figure S8. of Perilipin-2 modulates dietary fat-induced microbial global gene expression profiles in the mouse intestine

Rarefaction analysis of annotated mRNA reads. Recovery of species (A) and enzymes (B) with increasing numbers of annotated mRNA reads (reads mapped to known transcripts) indicate that sequencing depth for each sample was sufficient to recover the vast majority of species and enzymes present within each of the 16 samples. Rarefaction analysis was performed using R. (PDF 16825 kb

DataSheet1_Phenylalanine hydroxylase mRNA rescues the phenylketonuria phenotype in mice.docx

Phenylketonuria (PKU) is an inborn error of metabolism caused by a deficiency in functional phenylalanine hydroxylase (PAH), resulting in accumulation of phenylalanine (Phe) in patients’ blood and organs. Affected patients encounter severe developmental delay, neurological deficits, and behavioral abnormalities when not treated. Early diagnosis and treatment are extremely important; newborn screening programs have been implemented in most countries to ensure early identification of patients with PKU. Despite available treatment options, several challenges remain: life-long adherence to a strict diet, approval of some medications for adults only, and lack of response to these therapies in a subpopulation of patients. Therefore, there is an urgent need for treatment alternatives. An mRNA-based approach tested in PKU mice showed a fast reduction in the accumulation of Phe in serum, liver and brain, the most significant organ affected. Repeated injections of LNP-formulated mouse PAH mRNA rescued PKU mice from the disease phenotype for a prolonged period of time. An mRNA-based approach could improve the quality of life tremendously in PKU patients of all ages by replacing standard-of-care treatments.</p

Additional file 1 of Functional intraepithelial lymphocyte changes in inflammatory bowel disease and spondyloarthritis have disease specific correlations with intestinal microbiota

Supplementary Data. (DOCX 22 kb