Impossible to 'wean' when more aid is needed

Kirigia and Diarra-Nama from the WHO Regional Office for Africa say that funding for health in the WHO Africa Region remains inadequate and that, in some countries, is significantly dependent on donor funding. They propose five strategies for these countries to “wean themselves off” donor funding. While each of the proposed strategies might have some value in itself, they will not succeed in the double objective the authors set: to wean countries from depending upon international health aid and to achieve the US$34 per person annual health expenditure target suggested by the Commission on Macroeconomics and Health1 – an amount that must now be adjusted to US$ 40 due to inflation.

An assessment of wheat (Triticum aestivum L.) genotypes under saline and waterlogged compacted soil conditions, I: grain yield and yield components

A pot experiment was conducted to study effects of salinity and waterlogging under soil compaction conditions on grain yield and yield components of wheat. Treatments were arranged in a factorial layout assigned to a randomized complete design with three replications. Treatment combinations included: two sets of compaction levels, i.e. non-compacted and compacted soil; four abiotic stresses, i.e. non-saline aerobic (untreated silt loam texture soil having ECe = 3 dS m-1); saline × aerobic (S) (ECe 15 dS m-1); saline × waterlogged (S×W); and waterlogged alone (W) were applied; and two Iranian wheat genotypes i.e. Kouhdasht and Tajan. Compaction was achieved by dropping a 5 kg weight, 20 times from 70 cm height on a wooden block placed on top of soil-filled pots. In non-waterlogged treatments, soil water was maintained at 70% of available water holding capacity (AWHC). Waterlogging was achieved by maintaining water up to 110% of the soil’s AWHC for 25 days during tillering stage. Compaction significantly intensified effect of all other treatments, except waterlogging, on grain yield and yield components of wheat genotypes as compared to control. S×W caused significantly higher reduction in grain yield and yield components for both genotypes than other treatments

An assessment of wheat (Triticum aestivum L.) genotypes under saline and waterlogged compacted soil conditions, II: leaf ion concentrations

A pot experiment was conducted to study effects of salinity and waterlogging under soil compaction conditions on grain yield and yield components of wheat. Treatments were arranged in a factorial layout assigned to a randomized complete design with three replications. Treatment combinations included: two sets of compaction levels, i.e. non-compacted and compacted soil; four abiotic stresses, i.e. non-saline aerobic (untreated silt loam texture soil having ECe = 3 dS m-1); saline × aerobic (S) (ECe 15 dS m-1); saline × waterlogged (S×W); and waterlogged alone (W) were applied; and two Iranian wheat genotypes i.e. Kouhdasht and Tajan. Compaction was achieved by dropping a 5 kg weight, 20 times from 70 cm height on a wooden block placed on top of soil-filled pots. In non-waterlogged treatments, soil water was maintained at 70% of available water holding capacity (AWHC). Waterlogging was achieved by maintaining water up to 110% of the soil’s AWHC for 25 days during tillering stage. S×W caused significantly higher reduction in K+ concentration for both genotypes than other treatments. S×W also resulted in higher leaf Na+ and Cl- concentrations in comparison to other treatments. Kouhdasht maintained significantly higher K + concentration and K+: Na+ ratio at S and S×W treatments than that Tajan (under both non-compacted and compacted soil conditions)

Perceived distance, shape and size

AbstractIf distance, shape and size are judged independently from the retinal and extra-retinal information at hand, different kinds of information can be expected to dominate each judgement, so that errors in one judgement need not be consistent with errors in other judgements. In order to evaluate how independent these three judgements are, we examined how adding information that improves one judgement influences the others. Subjects adjusted the size and the global shape of a computer-simulated ellipsoid to match a tennis ball. They then indicated manually where they judged the simulated ball to be. Adding information about distance improved the three judgements in a consistent manner, demonstrating that a considerable part of the errors in all three judgements were due to misestimating the distance. Adding information about shape that is independent of distance improved subjects’ judgements of shape, but did not influence the set size or the manually indicated distance. Thus, subjects ignored conflicts between the cues when judging the shape, rather than using the conflicts to improve their estimate of the ellipsoid’s distance. We conclude that the judgements are quite independent, in the sense that no attempt is made to attain consistency, but that they do rely on some common measures, such as that of distance

Mass spectrometry and ribosome profiling, a perfect combination towards a more comprehensive identification strategy of true in vivo protein forms

An increasing number of studies involve integrative analysis of gene and protein expression data, taking advantage of new technologies such as next-generation transcriptome sequencing (RNA-Seq) and highly sensitive mass spectrometry (MS). Recently, a strategy, termed ribosome profiling, based on deep sequencing of ribosome-protected mRNA fragments, indirectly monitoring protein synthesis, has been described. In contrast to routinely employed protein databases in proteomics searches, RIBO-seq derived data gives a more representative expression state and accounts for sequence variation information and alternative translation initiation. To verify the potential of ribosome profiling in providing us with a true snapshot of the translational landscape, we devised a proteogenomic approach generating a database of translation products based on ribosome profiling experiments. The raw and untreated RIBO-seq data is analyzed for both splice isoforms and single nucleotide polymorphisms, as such taking into account transcriptional variation. Next to that, RIBO-seq data for translation start site discovery (treated with harringtonine, lactomidomycin or puromycin) is used to obtain a genome wide blueprint of all possible translation initiation sites and as such taking into account translation variation. By adding protein-DB annotation to the genomic RIBO-seq derived data and after in silico translation a protein database is constructed reflecting the full complexity of the proteome. Using a first version of our proteogenomic approach on an undifferentiated mouse embryonic stem cell line (E14) we could demonstrate an increase of the overall protein identification rate with 2.5% as compared to only searching UniProtKB-SwissProt. Furthermore, identification of N-terminal COFRADIC data resulted in detection of 16 alternative start sites giving rise to N-terminally extended protein variants besides the identification of four translated uORFs

The Health Systems Funding Platform : is this where we thought we were going?

Background: In March 2009, the Task Force for Innovative International Financing for Health Systems recommended "a health systems funding platform for the Global Fund, GAVI Alliance, the World Bank and others to coordinate, mobilize, streamline and channel the flow of existing and new international resources to support national health strategies." Momentum to establish the Health Systems Funding Platform was swift, with the World Bank convening a Technical Workshop on Health Systems Strengthening (HSS), and serial meetings organized to progress the agenda. Despite its potential significance, there has been little comment in peer-reviewed literature, though some disquiet in the international development community around the scope of the Platform and the capacity of the partners, which appears disproportionate to the available information. Methods: This case study uses documentary analysis, participant observation and 24 in-depth interviews to examine the processes of development and key issues raised by the Platform. Results: The findings show a fluid and volatile process, with debate over whether ongoing engagement in HSS by Global Fund and GAVI represents a dilution of organizational focus, risking ongoing support, or a paradigm shift that facilitates the achievement of targeted objectives, builds systems capacity, and will attract additional resources. Uncertainty in the development of the Platform reflects the flexibility of the recently formed global health initiatives, and the instability of donor commitments, particularly in the current financial climate. But implicit in the conflict is tension between key global stakeholders over defining and ownership of the health systems agenda. Conclusions: The tensions appear to have been resolved through a focus on national planning, applying International Health Partnership principles, though the global financial crisis and key personnel changes may yet alter outcomes. Despite its dynamic evolution, the Platform may offer an incremental path towards increasing integration around health systems, that has not been previously possible