A novel lice control method: photochemical treatment (Photolice)

Control of salmon lice has long been the biggest challenge for the further development of a sustainable industry. Preventive measures are expected to play an increasing role, but experience suggests that drug treatment will still be an important tool in an effective lice strategy. Today's control options are limited by unwanted environmental effects or resistance issues linked to the use of chemicals. This project has tested whether light-activated chemicals used in e.g. human cancer medicine could be an effective and environmentally friendly treatment method against salmon lice. The project consisted of three parts, which first examined the direct effect of substances on salmon lice (screening phase), after which selected substances were tested on lice-infected fish and finally on organisms from the environment around the cage. Nine different compounds were tested, where three of the tested compounds induced mortality when applied directly to salmon lice but did not induce loss of salmon lice when applied to infected fish. The tested compounds had no negative effects on fish welfare, nor did they induce mortality or change the behavior of rock-pool shrimps, which were used as an example of an organism that might be affected. We conclude that the use of photoactivated compounds may be possible to eliminate salmon lice from fish in aquaculture environments, but further studies are necessary.A novel lice control method: photochemical treatment (Photolice)publishedVersio

Disentangling the key drivers of salmon louse Lepeophtheirus salmonis fecundity using multiyear field samples

Planktonic salmon louse Lepeophtheirus salmonis salmonis larvae produced at sal - mon farms spread to and infect both wild and farmed salmonids. Understanding and forecasting the production and distribution of these larval stages from farms is critical to aquaculture management. However, model forecasts are based on available data and therefore include parameters with limited empirical support. This investigation examined salmon louse fecundity with a focus on batch egg clutch size by collecting lice from farmed Atlantic salmon Salmo salar at multiple farms and from wild Atlantic salmon and sea trout S. trutta captured at field sites throughout Norway. The data were analyzed with mixed effects models and total length of female lice was identified as the primary determinant of clutch size. Further analysis revealed that female louse total length is partially explained by temperature at sampling. However, if the temperature at sampling is spatially or temporally disconnected from rearing temperature, it may not be possible to predict the total length of a louse using temperature. The fecundity investigation further found that 66% of female lice on farmed salmon were sexually mature, and 10% of these were not egg-bearing. In comparison, 73% of adult female lice on sea trout were sexually mature, and 40% of these were not egg-bearing. Our results indicate that salmon louse production forecasts would be improved by incorporating female louse sexual maturity and a clutch size parameter that is related to total length of female lice.publishedVersio

Transcriptomic and targeted immune transcript analyses confirm localized skin immune responses in Atlantic salmon towards the salmon louse

Atlantic salmon (Salmo salar) are highly susceptible to infestations with the ectoparasite Lepeophtheirus salmonis, the salmon louse. Infestations elicit an immune response in the fish, but the response does not lead to parasite clearance, nor does it protect against subsequent infestations. It is, however, not known why the immune response is not adequate, possibly because the local response directly underneath the louse has been poorly evaluated. The present study describes the transcriptomic response by RNA sequencing of skin at the site of copepodid attachment. Analysing differentially expressed genes, 2864 were higher and 1357 were lower expressed at the louse attachment site compared to uninfested sites in the louse infested fish, while gene expression at uninfested sites were similar to uninfested control fish. The transcriptional patterns of selected immune genes were further detailed in three skin compartments/types: Whole skin, scales only and fin tissue. The elevation of pro-inflammatory cytokines and immune cell marker transcripts observed in whole skin and scale samples were not induced in fin, and a higher cytokine transcript level in scale samples suggest it can be used as a nonlethal sampling method to enhance selective breeding trials. Furthermore, the immune response was followed in both skin and anterior kidney as the infestation developed. Here, newly moulted preadult 1 stage lice induced a higher immune response than chalimi and adult lice. Overall, infestation with salmon louse induce a modest but early immune response with an elevation of mainly innate immune transcripts, with the response primarily localized to the site of attachment.publishedVersio

The salmon louse Lepeophtheirus salmonis (Copepoda: Caligidae) life cycle has only two chalimus stages

Each year the salmon louse (Lepeophtheirus salmonis Krøyer, 1838) causes multi-million dollar commercial losses to the salmon farming industry world-wide, and strict lice control regimes have been put in place to reduce the release of salmon louse larvae from aquaculture facilities into the environment. For half a century, the Lepeophtheirus life cycle has been regarded as the only copepod life cycle including 8 post-nauplius instars as confirmed in four different species, including L. salmonis. Here we prove that the accepted life cycle of the salmon louse is wrong. By observations of chalimus larvae molting in incubators and by morphometric cluster analysis, we show that there are only two chalimus instars: chalimus 1 (comprising the former chalimus I and II stages which are not separated by a molt) and chalimus 2 (the former chalimus III and IV stages which are not separated by a molt). Consequently the salmon louse life cycle has only six post-nauplius instars, as in other genera of caligid sea lice and copepods in general. These findings are of fundamental importance in experimental studies as well as for interpretation of salmon louse biology and for control and management of this economically important parasite.publishedVersio

In sea trout, the physiological response to salmon louse is stronger in female than in males

The aims of this study were to compare male and female sea trout (Salmo trutta) with respect to their hypo-osmoregulatory ability over a simulated migration to seawater and their clinical and physiological response to salmon louse (Lepeophtheirus salmonis) infection in seawater and over a simulated pre-mature return to freshwater. For this purpose, 2-year-old hatchery-reared male and female brown trout (F1 offspring of wild caught anadromous fish) were infected with salmon lice and measured for changes in plasma ions, glucose, lactate and osmolality and relative heart, liver and gonad sizes during a simulated seawater migration and thereafter a premature return to freshwater after 4 weeks in seawater (pre-adult louse). Un-infected trout served as control. Male trout used longer time to develop full hypo-osmoregulatory ability in seawater and showed a stronger response in plasma glucose and lactate following simulated premature return to freshwater, compared to female trout. Response to salmon louse was stronger in female trout, shown by stronger osmotic stress by chalimus (plasma Cl−) and pre-adult louse (plasma osmolality) and elevated relative liver size (hepatosomatic index) by pre-adult louse in female compared to male trout. Moreover, high plasma cortisol in infected female and low plasma cortisol in infected male trout produced a significant treatment–sex interaction on plasma cortisol. Lice infection intensity was initially higher in male (0.18 lice g−1) compared to female trout (0.11 lice g−1) at the chalimus stage, but equal between sexes at the pre-adult stage (male 0.15 and female 0.17 lice g−1). This study showed that female trout were better adapted for changes in water salinity, while male trout were more robust against salmon louse infection. These results suggests that the elevated salmon louse infection pressure generated by salmon farming have strong and unexplored negative effects on wild sea trout populations. Further research on this topic is vital for the conservation of wild sea trout populations.publishedVersio

RNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonis

Retrograde transport of proteins from the endoplasmic reticulum to the Golgi is an essential part of the secretory pathway that all newly synthesised secreted and membrane proteins in eukaryotic cells undergo. The aim of this study was to characterise two components of the retrograde transport pathway in the parasitic copepod Lepeophtheirus salmonis (salmon louse) on a molecular and functional level. LsKDELR and LsCOPB2 were confirmed to be the salmon louse homologues of the chosen target proteins by sequence similarity. Ontogenetic analysis by qRT-PCR revealed the highest expression levels of both genes in adult females and the earliest larval stage. LsKDELR and LsCOPB2 localisation in adult females was detected by immunofluorescence and in situ hybridisation, respectively. Both LsKDELR and LsCOPB2 were found in the ovaries, the oocytes and the gut. LsKDELR and LsCOPB2 were knocked down by RNA interference in preadult females, which was confirmed by qRT-PCR. LsCOPB2 knockdown lice had a significantly higher mortality and failed to develop normally, while both LsCOPB2 and LsKDELR knockdown caused disturbed digestion and the absence of egg strings. This shows the potential of LsKDELR and LsCOPB2 as suitable target candidates for new pest control methods

RNA interference mediated knockdown of the KDEL receptor and COPB2 inhibits digestion and reproduction in the parasitic copepod Lepeophtheirus salmonis

Retrograde transport of proteins from the endoplasmic reticulum to the Golgi is an essential part of the secretory pathway that all newly synthesised secreted and membrane proteins in eukaryotic cells undergo. The aim of this study was to characterise two components of the retrograde transport pathway in the parasitic copepod Lepeophtheirus salmonis (salmon louse) on a molecular and functional level. LsKDELR and LsCOPB2 were confirmed to be the salmon louse homologues of the chosen target proteins by sequence similarity. Ontogenetic analysis by qRT-PCR revealed the highest expression levels of both genes in adult females and the earliest larval stage. LsKDELR and LsCOPB2 localisation in adult females was detected by immunofluorescence and in situ hybridisation, respectively. Both LsKDELR and LsCOPB2 were found in the ovaries, the oocytes and the gut. LsKDELR and LsCOPB2 were knocked down by RNA interference in preadult females, which was confirmed by qRT-PCR. LsCOPB2 knockdown lice had a significantly higher mortality and failed to develop normally, while both LsCOPB2 and LsKDELR knockdown caused disturbed digestion and the absence of egg strings. This shows the potential of LsKDELR and LsCOPB2 as suitable target candidates for new pest control methods.acceptedVersio

Characterization of a novel RXR receptor in the salmon louse (Lepeophtheirus salmonis, Copepoda) regulating growth and female reproduction

Nuclear receptors have crucial roles in all metazoan animals as regulators of gene transcription. A wide range of studies have elucidated molecular and biological significance of nuclear receptors but there are still a large number of animals where the knowledge is very limited. In the present study we have identified an RXR type of nuclear receptor in the salmon louse (Lepeophtheirus salmonis) (i.e. LsRXR). RXR is one of the two partners of the Ecdysteroid receptor in arthropods, the receptor for the main molting hormone 20-hydroxyecdysone (E20) with a wide array of effects in arthropods

Molekylære studier av lakselus : Molecular studies of salmon lice

Salmon lice are an important threat to wild populations of salmon and sea trout and a growing problem in aquaculture. At the Institute of Marine Research, a molecular characterization of salmon lice is performed to provide a detailed study of salmon lice biology

Lakselus – risikovurdering og kunnskapsstatus 2023 — Dødelighet og negative effekter hos laksefisk som følge av lakselus

Risikovurderingen omfatter dødelighet på utvandrende postsmolt laks og negative effekter på sjøørret og sjørøye som følge av lakselussmitte. Vurderingene baserer seg på konsekvensen av at villfisken blir smittet med lakselus fra oppdrett og toleransen villfisken har for lakselussmitte. Til grunn for vurderingen av lakselussmitte har vi benyttet kunnskap om utslipp av lakselus og i hvilken grad det er overlapp med tilstedeværelse av villfisken i tid og rom. Utslipp av lakselus er beregnet fra antallet av voksne hunnlus på oppdrettsfisk, antall fisk i anleggene og temperatur innrapportert fra oppdrettsanlegg. Vi har benyttet modeller for å simulere spredningen av lakseluslarver fra oppdrettsanlegg. Inkludert her er beregning av hvordan vannstrømmene transporterer lus, hvordan lakseluslarvene utvikler seg som funksjon av vanntemperatur og hvordan de beveger seg nedover i områder med lav saltholdighet. Hvor og når fisken blir eksponert for lakselus er basert på estimerte utvandringstider, og utvandringsruter som i stor grad antar at laksen svømmer målrettet mot havet, mens for sjøørret og sjørøye vurderes utvandringsperiode, beiteområde og beiteperiode.  Lakselus – risikovurdering og kunnskapsstatus 2023 — Dødelighet og negative effekter hos laksefisk som følge av lakseluspublishedVersio