Water Use Efficiency in Chilean and Argentine Humid Temperate Grass-Legume Pastures

At two sites in Argentina and Chile five levels of water input were applied to four sown pastures of varying ages during spring and summer. The pastures consisted principally of C3 grasses and legumes, some of which were sown such as Lolium perenne, Trifolium repens, Dactylis glomerata. Dry matter (DM) production was measured and related to the estimated total evapotranspiration (ET): responses were both highly linear. Both responses to ET and absolute yields were higher at the Argentinian than at the Chilean site: respectively 10.7 and 15.2 kg DM/mm water evapotranspired. Nevertheless the calculated indices of sensitivity (Ky) of Doorenbos and Kassam (1979) were similar for the two sites, indicating a similar priority for irrigation in terms of expected responses

Internationalisation, cultural distance and country characteristics: a Bayesian analysis of SME's financial performance

Relying on the accounting data of a panel of 403 Italian manufacturing SMEs collected over a period of 5 years, we find results suggesting that multinationality per se does not impact on the economic performance of international small and medium sized firms. It is the characteristics of the country selected i.e. the political hazard, the financial stability and the economic performance that significantly influence SMEs financial performance. The management implication for small and medium sized firms selecting and entering new geographic markets is significant, since our results show that for SMEs it is the market selection process that really matters and not the degree of multinationality

FATZ, a filamin-, actinin-, and telethonin-binding protein of the Z-disc of skeletal muscle

We report the identification and characterization of a novel 32-kDa protein expressed in skeletal muscle and located in the Z-disc of the sarcomere. We found that this protein binds to three other Z-disc proteins; therefore, we have-named it FATZ, gamma -filamin/ABP-L, alpha -actinin and telethonin binding protein of the Z-disc. From yeast two-hybrid experiments we are able to show that the SR3-SR4 domains of alpha -actinin 2 are required to bind the COOH-terminal region of the FATZ as does gamma -filamin/ABP-L, Furthermore, by using a glutathione S-transferase overlay assay we find that FATZ also binds telethonin. The level of FATZ protein in muscle cells increases during differentiation, being clearly detectable before the onset of myosin, Although FATZ has no known interaction domains, it would appear to be involved in a complex network of interactions with other Z-band components. On the basis of the information known about its binding partners, we could envisage a central role for FATZ in the: myofibrillogenesis, After screening our muscle expressed sequence tag data base and the public expressed sequence tag data bases, we were able to assemble two other muscle transcripts that show a high level of identity with FATZ in two different domains. Therefore, FATZ may be the first member of a small family of novel muscle proteins

Large-scale response of the Eastern Mediterranean thermohaline circulation to African monsoon intensification during sapropel S1 formation

The formation of Eastern Mediterranean sapropels has periodically occurred during intensification of northern hemisphere monsoon precipitation over North Africa. However, the large-scale response of the Eastern Mediterranean thermohaline circulation during these monsoon-fuelled freshening episodes is poorly constrained. Here, we investigate the formation of the youngest sapropel (S1) along an across-slope transect in the Adriatic Sea. Foraminifera-based oxygen index, redox-sensitive elements and biogeochemical parameters reveal – for the first time – that the Adriatic S1 was synchronous with the deposition of south-eastern Mediterranean S1 beds. Proxies of paleo thermohaline currents indicate that the bottom-hugging North Adriatic Dense Water (NAdDW) suddenly decreased at the sapropel onset simultaneously with the maximum freshening of the Levantine Sea during the African Humid Period. We conclude that the lack of the “salty” Levantine Intermediate Water hampered the preconditioning of the northern Adriatic waters necessary for the NAdDW formation prior to the winter cooling. Consequently, a weak NAdDW limited in turn the Eastern Mediterranean Deep Water (EMDWAdriatic) formation with important consequences for the ventilation of the Ionian basin as well. Our results highlight the importance of the Adriatic for the deep water ventilation and the interdependence among the major eastern Mediterranean water masses whose destabilization exerted first-order control on S1 deposition

Large-scale response of the Eastern Mediterranean thermohaline circulation to African monsoon intensification during sapropel S1 formation

This study was supported by Shell International Exploration and Production Inc. We thank the R/V URANIA crew for at sea assistance. This is the ISMAR contribution n. 1914. We thank Dr. L. Capotondi and Dr. L. Vigliotti for their constructive comments on the first draft of the manuscript. We also thank Dr. Daria Pasqual (University of Padova, Dept. of Geosciences) for her assistance in XRF analyses. We thank two anonymous reviewers and the Editor H. Bauch for their constructive comments. We also acknowledge Prof. Gerhard Schmiedl (Universität Hamburg) and Associate Prof. Syee Weldeab (Earth Science, UC Santa Barbara) for providing published data used in this study.Peer reviewedPostprin

Intratumor Regulatory Noncytotoxic NK Cells in Patients with Hepatocellular Carcinoma

Previous studies support the role of natural killer (NK) cells in controlling hepatocellular carcinoma (HCC) progression. However, ambiguity remains about the multiplicity and the role of different NK cell subsets, as a pro-oncogenic function has been suggested. We performed phenotypic and functional characterization of NK cells infiltrating HCC, with the corresponding nontumorous tissue and liver from patients undergoing liver resection for colorectal liver metastasis used as controls. We identified a reduced number of NK cells in tumors with higher frequency of CD56(BRIGHT)CD16(-) NK cells associated with higher expression of NKG2A, NKp44, and NKp30 and downregulation of NKG2D. Liver-resident (CXCR6(+)) NK cells were reduced in the tumors where T-bet(hi)Eomes(lo) expression was predominant. HCCs showed higher expression of CD49a with particular enrichment in CD49a(+)Eomes(+) NK cells, a subset typically represented in the decidua and playing a proangiogenic function. Functional analysis showed reduced TNF-alpha production along with impaired cytotoxic capacity that was inversely related to CXCR6(-), T-bet(hi)Eomes(lo), and CD49a(+)Eomes(+) NK cells. In conclusion, we identified a subset of NK cells infiltrating HCC, including non-liver-resident cells that coexpressed CD49a and Eomes and showed reduced cytotoxic potential. This NK cell subset likely plays a regulatory role in proangiogenic function

The Late Pleistocene Po River lowstand wedge in the Adriatic Sea: Controls on architecture variability and sediment partitioning

Although facies and stratal geometries of continental margin successions can be defined in detail based on subsurface and outcrop studies, most studies lack the high-resolution age control needed to constrain the time scale of formation of such successions and infer their external forcing mechanisms. Our work on the Po River Lowstand Wedge (PRLW) indicates that deposition rates are surprisingly high with the entire 350-m-thick succession being deposited in less than 17,000 years, and with individual clinothems recording time periods ranging from 400 to 4700 years. The PRLW preserves a high-resolution record of stacked, deltaic shelf-edge clinothems deposited during the Last Glacial Maximum (31.8–14.4 ky BP) in the Adriatic basin (Mediterranean Sea). We investigated clinothem internal geometry, stacking patterns, and facies distributions to infer the main controls on their growth by integrating seismic reflection data with seismic facies attributes and paleoenvironmental proxies. The stratigraphic framework of the shelf-edge clinothems was then related to major paleoenvironmental shifts during the last glacial cycle and driven by eustatic and climatic changes. Within the PRLW, we recognized three distinctive types of 100's-m-high shelf-edge clinothems, type A, type B and type C, each with diagnostic topset geometries, shelf-edge trajectories, and associated distal basin-fill deposits. These elemental clinothem types stack into two Clinothem Sets. Clinothem Set 1, with essentially flat to slightly descending shelf-edge trajectory, is composed of stacked types A and B clinothems, and records the direct influence of river flux leading to dysoxic conditions on the bottom of the basin. In particular, clinothem accumulation rates were as much as 200 km3/ky in some of the type B clinothems. Clinothem Set 2, showing ascending shelf-edge trajectory, records an aggradational stacking coupled with a retreat of the river-entry points with benthic fauna assemblages that reflect the influence of peaks in freshwater discharge. Whereas Clinothem Set 1 developed under perturbations of river supply linked to the multi-scale waxing and waning of glaciers during an interval dominated by eustatic fall, Clinothem Set 2 reflects the main thawing of glaciers concomitant to the first phase of the eustatic rise. From a sequence stratigraphic perspective, Clinothem Set 1 is interpreted as staked high-frequency sequences, while Clinothem Set 2 represents a stack of high-frequency parasequences. The high-resolution age control from boreholes and seismic data enabled us to relate stratal character to independently constrained environmental proxies: this revealed how the evolution of a shelf-edge system intricately convolves the influences of both global (eustacy) and regional (climate-driven supply fluctuations) controls, both at sub-Milankovitch scales. Finally, the thickness, geometry, and stacking patterns of the centennial to millennial clinothems of the PRLW vary in systematic ways resulting in geometries that closely resemble those of ancient shelf-edge systems, and offering the PRLW as a sub-modern analogue. Our observations also reinforce the focus of the classic sequence-stratigraphic approach on analyzing surfaces and their geometric relations and not on time duration or formation mechanisms

A novel DSP zebrafish model reveals training- and drug-induced modulation of arrhythmogenic cardiomyopathy phenotypes

Arrhythmogenic cardiomyopathy (AC) is an inherited disorder characterized by progressive loss of the ventricular myocardium causing life-threatening ventricular arrhythmias, syncope and sudden cardiac death in young and athletes. About 40% of AC cases carry one or more mutations in genes encoding for desmosomal proteins, including Desmoplakin (Dsp). We present here the first stable Dsp knock-out (KO) zebrafish line able to model cardiac alterations and cell signalling dysregulation, characteristic of the AC disease, on which environmental factors and candidate drugs can be tested. Our stable Dsp knock-out (KO) zebrafish line was characterized by cardiac alterations, oedema and bradycardia at larval stages. Histological analysis of mutated adult hearts showed reduced contractile structures and abnormal shape of the ventricle, with thinning of the myocardial layer, vessels dilation and presence of adipocytes within the myocardium. Moreover, TEM analysis revealed “pale”, disorganized and delocalized desmosomes. Intensive physical training protocol caused a global worsening of the cardiac phenotype, accelerating the progression of the disease. Of note, we detected a decrease of Wnt/β-catenin signalling, recently associated with AC pathogenesis, as well as Hippo/YAP-TAZ and TGF-β pathway dysregulation. Pharmacological treatment of mutated larvae with SB216763, a Wnt/β-catenin agonist, rescued pathway expression and cardiac abnormalities, stabilizing the heart rhythm. Overall, our Dsp KO zebrafish line recapitulates many AC features observed in human patients, pointing at zebrafish as a suitable system for in vivo analysis of environmental modulators, such as the physical exercise, and the screening of pathway-targeted drugs, especially related to the Wnt/β-catenin signalling cascade

The use of porcine small intestinal submucosa mesh (SURGISIS) as a pelvic sling in a man and a woman with previous pelvic surgery: two case reports

<p>Abstract</p> <p>Introduction</p> <p>Closing the pelvic peritoneum to prevent the small bowel dropping into the pelvis after surgery for locally recurrent rectal cancer is important to prevent adhesions deep in the pelvis or complications of adjuvant radiotherapy. Achieving this could be difficult because sufficient native tissue is unavailable; we report on the use of small intestine submucosa extra-cellular matrix mesh in the obliteration of the pelvic brim.</p> <p>Case presentation</p> <p>We describe two cases in which submucosa extra-cellular matrix mesh was used to obliterate the pelvic brim following resection of a recurrent rectal tumour; the first patient, a 78-year-old Caucasian man, presented with small bowel obstruction caused by adhesions to a recurrent rectal tumour. The second patient, an 84-year-old Caucasian woman, presented with vaginal discharge caused by an entero-vaginal fistula due to a recurrent rectal tumour.</p> <p>Conclusion</p> <p>We report on the use of submucosa extra-cellular matrix mesh as a pelvic sling in cases where primary closure of the pelvic peritoneum is unfeasible. Its use had no infective complications and added minimal morbidity to the postoperative period. This is an original case report that would be of interest to general and colorectal surgeons.</p

Efficient clofilium tosylate-mediated rescue of POLG-related disease phenotypes in zebrafish

The DNA polymerase gamma (Polg) is a nuclear-encoded enzyme involved in DNA replication in animal mitochondria. In humans, mutations in the POLG gene underlie a set of mitochondrial diseases characterized by mitochondrial DNA (mtDNA) depletion or deletion and multiorgan defects, named POLG disorders, for which an effective therapy is still needed. By applying antisense strategies, ENU- and CRISPR/Cas9-based mutagenesis, we have generated embryonic, larval-lethal and adult-viable zebrafish Polg models. Morphological and functional characterizations detected a set of phenotypes remarkably associated to POLG disorders, including cardiac, skeletal muscle, hepatic and gonadal defects, as well as mitochondrial dysfunctions and, notably, a perturbed mitochondria-to-nucleus retrograde signaling (CREB and Hypoxia pathways). Next, taking advantage of preliminary evidence on the candidate molecule Clofilium tosylate (CLO), we tested CLO toxicity and then its efficacy in our zebrafish lines. Interestingly, at well tolerated doses, the CLO drug could successfully rescue mtDNA and Complex I respiratory activity to normal levels, even in mutant phenotypes worsened by treatment with Ethidium Bromide. In addition, the CLO drug could efficiently restore cardio-skeletal parameters and mitochondrial mass back to normal values. Altogether, these evidences point to zebrafish as a valuable vertebrate organism to faithfully phenocopy multiple defects detected in POLG patients. Moreover, this model represents an excellent platform to screen, at the whole-animal level, candidate molecules with therapeutic effects in POLG disorders