978 research outputs found
Increased toxin expression in a Clostridium difficile mfd mutant
BACKGROUND: The symptoms of Clostridium difficile infection are mediated primarily by two toxins, TcdA and TcdB, the expression of which is governed by a multitude of factors including nutrient availability, growth phase and cell stress. Several global regulators have been implicated in the regulation of toxin expression, such as CcpA and CodY. RESULTS: During attempts to insertionally inactivate a putative secondary cell wall polysaccharide synthesis gene, we obtained several mutants containing off-target insertions. One mutant displayed an unusual branched colony morphology and was investigated further. Marker recovery revealed an insertion in mfd, a gene encoding a transcription-coupled repair factor. The mfd mutant exhibited pleiotropic effects, in particular increased expression of both toxin A and B (TcdA and TcdB) compared to the parental strain. Western blotting and cellular cytotoxicity assays revealed increased expression across all time points over a 24Â h period, with inactivation of mfd resulting in at least a 10 fold increase in cell cytotoxicity. qRT-PCR demonstrated the upregulation of both toxins occurred on a transcriptional level. All effects of the mfd mutation were complemented by a plasmid-encoded copy of mfd, showing the effects are not due to polar effects of the intron insertion or to second site mutations. CONCLUSIONS: This study adds Mfd to the repertoire of factors involved in regulation of toxin expression in Clostridium difficile. Mfd is known to remove RNA polymerase molecules from transcriptional sites where it has stalled due to repressor action, preventing transcriptional read through. The consistently high levels of toxin in the C. difficile mfd mutant indicate this process is inefficient leading to transcriptional de-repression
Comparing [C II], H I, and CO dynamics of nearby galaxies
The HI and CO components of the interstellar medium (ISM) are usually used to derive the dynamical mass M-dyn of nearby galaxies. Both components become too faint to be used as a tracer in observations of high-redshift galaxies. In those cases, the 158 mu m line of atomic carbon ([CII]) may be the only way to derive M-dyn. As the distribution and kinematics of the ISM tracer affects the determination of M-dyn, it is important to quantify the relative distributions of HI, CO, and [CII]. HI and CO are well-characterized observationally, however, for [CII] only very few measurements exist. Here we compare observations of CO, HI, and [CII] emission of a sample of nearby galaxies, drawn from the HERACLES, THINGS, and KINGFISH surveys. We find that within R-25, the average [CII] exponential radial profile is slightly shallower than that of the CO, but much steeper than the HI distribution. This is also reflected in the integrated spectrum ("global profile"), where the [CII] spectrum looks more like that of the CO than that of the HI. For one galaxy, a spectrally resolved comparison of integrated spectra was possible; other comparisons were limited by the intrinsic line-widths of the galaxies and the coarse velocity resolution of the [CII] data. Using high-spectral-resolution SOFIA [CII] data of a number of star forming regions in two nearby galaxies, we find that their [CII] linewidths agree better with those of the CO than the HI. As the radial extent of a given ISM tracer is a key input in deriving M-dyn from spatially unresolved data, we conclude that the relevant length-scale to use in determining M-dyn based on [CII] data, is that of the well-characterized CO distribution. This length scale is similar to that of the optical disk
Timescales of Massive Human Entrainment
The past two decades have seen an upsurge of interest in the collective
behaviors of complex systems composed of many agents entrained to each other
and to external events. In this paper, we extend concepts of entrainment to the
dynamics of human collective attention. We conducted a detailed investigation
of the unfolding of human entrainment - as expressed by the content and
patterns of hundreds of thousands of messages on Twitter - during the 2012 US
presidential debates. By time locking these data sources, we quantify the
impact of the unfolding debate on human attention. We show that collective
social behavior covaries second-by-second to the interactional dynamics of the
debates: A candidate speaking induces rapid increases in mentions of his name
on social media and decreases in mentions of the other candidate. Moreover,
interruptions by an interlocutor increase the attention received. We also
highlight a distinct time scale for the impact of salient moments in the
debate: Mentions in social media start within 5-10 seconds after the moment;
peak at approximately one minute; and slowly decay in a consistent fashion
across well-known events during the debates. Finally, we show that public
attention after an initial burst slowly decays through the course of the
debates. Thus we demonstrate that large-scale human entrainment may hold across
a number of distinct scales, in an exquisitely time-locked fashion. The methods
and results pave the way for careful study of the dynamics and mechanisms of
large-scale human entrainment.Comment: 20 pages, 7 figures, 6 tables, 4 supplementary figures. 2nd version
revised according to peer reviewers' comments: more detailed explanation of
the methods, and grounding of the hypothese
Resolving the far-IR line deficit : photoelectric heating and far-IR line cooling in NGC 1097 and NGC 4559
The physical state of interstellar gas and dust is dependent on the processes which heat and cool this medium. To probe heating and cooling of the interstellar medium over a large range of infrared surface brightness, on sub-kiloparsec scales, we employ line maps of [C II] 158 mu m, [O I] 63 mu m, and [N II] 122 mu m in NGC 1097 and NGC 4559, obtained with the Photodetector Array Camera & Spectrometer on board Herschel. We matched new observations to existing Spitzer Infrared Spectrograph data that trace the total emission of polycyclic aromatic hydrocarbons (PAHs). We confirm at small scales in these galaxies that the canonical measure of photoelectric heating efficiency, ([C II] + [O I])/TIR, decreases as the far-infrared (far-IR) color, nu f(nu)(70 mu m) nu f(nu)(100 mu m), increases. In contrast, the ratio of far-IR cooling to total PAH emission, ([C II] + [O I])/PAH, is a near constant similar to 6% over a wide range of far-IR color, 0.5 , derived from models of the IR spectral energy distribution. Emission from regions that exhibit a line deficit is characterized by an intense radiation field, indicating that small grains are susceptible to ionization effects. We note that there is a shift in the 7.7/11.3 mu m PAH ratio in regions that exhibit a deficit in ([C II] + [O I])/PAH, suggesting that small grains are ionized in these environments
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COMPARING [C â„], H i, and CO DYNAMICS of NEARBY GALAXIES
The HI and CO components of the interstellar medium (ISM) are usually used to
derive the dynamical mass M_dyn of nearby galaxies. Both components become too
faint to be used as a tracer in observations of high-redshift galaxies. In
those cases, the 158 m line of atomic carbon [CII] may be the only way to
derive M_dyn. As the distribution and kinematics of the ISM tracer affects the
determination of M_dyn, it is important to quantify the relative distributions
of HI, CO and [CII]. HI and CO are well-characterised observationally, however,
for [CII] only very few measurements exist. Here we compare observations of CO,
HI, and [CII] emission of a sample of nearby galaxies, drawn from the HERACLES,
THINGS and KINGFISH surveys. We find that within R_25, the average [CII]
exponential radial profile is slightly shallower than that of the CO, but much
steeper than the HI distribution. This is also reflected in the integrated
spectrum ("global profile"), where the [CII] spectrum looks more like that of
the CO than that of the HI. For one galaxy, a spectrally resolved comparison of
integrated spectra was possible; other comparisons were limited by the
intrinsic line-widths of the galaxies and the coarse velocity resolution of the
[CII] data. Using high-spectral-resolution SOFIA [CII] data of a number of star
forming regions in two nearby galaxies, we find that their [CII] linewidths
agree better with those of the CO than the HI. As the radial extent of a given
ISM tracer is a key input in deriving M_dyn from spatially unresolved data, we
conclude that the relevant length-scale to use in determining M_dyn based on
[CII] data, is that of the well-characterised CO distribution. This length
scale is similar to that of the optical disk.This is the accepted manuscript. It is currently embargoed pending publication
Sideband Cooling Micromechanical Motion to the Quantum Ground State
The advent of laser cooling techniques revolutionized the study of many
atomic-scale systems. This has fueled progress towards quantum computers by
preparing trapped ions in their motional ground state, and generating new
states of matter by achieving Bose-Einstein condensation of atomic vapors.
Analogous cooling techniques provide a general and flexible method for
preparing macroscopic objects in their motional ground state, bringing the
powerful technology of micromechanics into the quantum regime. Cavity opto- or
electro-mechanical systems achieve sideband cooling through the strong
interaction between light and motion. However, entering the quantum regime,
less than a single quantum of motion, has been elusive because sideband cooling
has not sufficiently overwhelmed the coupling of mechanical systems to their
hot environments. Here, we demonstrate sideband cooling of the motion of a
micromechanical oscillator to the quantum ground state. Entering the quantum
regime requires a large electromechanical interaction, which is achieved by
embedding a micromechanical membrane into a superconducting microwave resonant
circuit. In order to verify the cooling of the membrane motion into the quantum
regime, we perform a near quantum-limited measurement of the microwave field,
resolving this motion a factor of 5.1 from the Heisenberg limit. Furthermore,
our device exhibits strong-coupling allowing coherent exchange of microwave
photons and mechanical phonons. Simultaneously achieving strong coupling,
ground state preparation and efficient measurement sets the stage for rapid
advances in the control and detection of non-classical states of motion,
possibly even testing quantum theory itself in the unexplored region of larger
size and mass.Comment: 13 pages, 7 figure
Circulating Fatty Acids and Risk of Coronary Heart Disease and Stroke: Individual Participant Data Meta-Analysis in Up to 16Â 126 Participants
BACKGROUND
We aimed at investigating the association of circulating fatty acids with coronary heart disease (CHD) and stroke risk.
METHODS AND RESULTS
We conducted an individualâparticipant data metaâanalysis of 5 UKâbased cohorts and 1 matched caseâcontrol study. Fatty acids (ie, omegaâ3 docosahexaenoic acid, omegaâ6 linoleic acid, monounsaturated and saturated fatty acids) were measured at baseline using an automated highâthroughput serum nuclear magnetic resonance metabolomics platform. Data from 3022 incident CHD cases (13 104 controls) and 1606 incident stroke cases (13 369 controls) were included. Logistic regression was used to model the relation between fatty acids and odds of CHD and stroke, adjusting for demographic and lifestyle variables only (ie, minimally adjusted model) or with further adjustment for other fatty acids (ie, fully adjusted model). Although circulating docosahexaenoic acid, but not linoleic acid, was related to lower CHD risk in the fully adjusted model (odds ratio, 0.85; 95% CI, 0.76â0.95 per standard unit of docosahexaenoic acid), there was evidence of high betweenâstudy heterogeneity and effect modification by study design. Stroke risk was consistently lower with increasing circulating linoleic acid (odds ratio for fully adjusted model, 0.82; 95% CI, 0.75â0.90). Circulating monounsaturated fatty acids were associated with higher CHD risk across all models and with stroke risk in the fully adjusted model (odds ratio, 1.22; 95% CI, 1.03â1.44). Saturated fatty acids were not related to increased CHD risk in the fully adjusted model (odds ratio, 0.94; 95% CI, 0.82â1.09), or stroke risk.
CONCLUSIONS
We found consistent evidence that linoleic acid was associated with decreased risk of stroke and that monounsaturated fatty acids were associated with increased risk of CHD. The different pattern between CHD and stroke in terms of fatty acids risk profile suggests future studies should be cautious about using composite events. Different study designs are needed to assess which, if any, of the associations observed is causal
Surgical procedures in the treatment of 784 infected THAs reported to the Norwegian Arthroplasty Register: Best survival with 2-stage exchange revision, but also good results with debridement and retention of the fixed implant
Association between clinically recorded alcohol consumption and initial presentation of 12 cardiovascular diseases: population based cohort study using linked health records
To investigate the association between alcohol consumption and cardiovascular disease at higher resolution by examining the initial lifetime presentation of 12 cardiac, cerebrovascular, abdominal, or peripheral vascular diseases among five categories of consumption.
Population based cohort study of linked electronic health records covering primary care, hospital admissions, and mortality in 1997-2010 (median follow-up six years).
CALIBER (ClinicAl research using LInked Bespoke studies and Electronic health Records).
1â937â360 adults (51% women), aged â„30 who were free from cardiovascular disease at baseline.
12 common symptomatic manifestations of cardiovascular disease, including chronic stable angina, unstable angina, acute myocardial infarction, unheralded coronary heart disease death, heart failure, sudden coronary death/cardiac arrest, transient ischaemic attack, ischaemic stroke, intracerebral and subarachnoid haemorrhage, peripheral arterial disease, and abdominal aortic aneurysm.
114â859 individuals received an incident cardiovascular diagnosis during follow-up. Non-drinking was associated with an increased risk of unstable angina (hazard ratio 1.33, 95% confidence interval 1.21 to 1.45), myocardial infarction (1.32, 1.24 to1.41), unheralded coronary death (1.56, 1.38 to 1.76), heart failure (1.24, 1.11 to 1.38), ischaemic stroke (1.12, 1.01 to 1.24), peripheral arterial disease (1.22, 1.13 to 1.32), and abdominal aortic aneurysm (1.32, 1.17 to 1.49) compared with moderate drinking (consumption within contemporaneous UK weekly/daily guidelines of 21/3 and 14/2 units for men and women, respectively). Heavy drinking (exceeding guidelines) conferred an increased risk of presenting with unheralded coronary death (1.21, 1.08 to 1.35), heart failure (1.22, 1.08 to 1.37), cardiac arrest (1.50, 1.26 to 1.77), transient ischaemic attack (1.11, 1.02 to 1.37), ischaemic stroke (1.33, 1.09 to 1.63), intracerebral haemorrhage (1.37, 1.16 to 1.62), and peripheral arterial disease (1.35; 1.23 to 1.48), but a lower risk of myocardial infarction (0.88, 0.79 to 1.00) or stable angina (0.93, 0.86 to 1.00).
Heterogeneous associations exist between level of alcohol consumption and the initial presentation of cardiovascular diseases. This has implications for counselling patients, public health communication, and clinical research, suggesting a more nuanced approach to the role of alcohol in prevention of cardiovascular disease is necessary.
clinicaltrails.gov (NCT01864031).This work was supported by the National Institute for Health Research (RP-PG-0407-10314), Wellcome Trust (WT 086091/Z/08/Z), the Medical Research Council prognosis research strategy (PROGRESS) Partnership (G0902393/99558), and awards to establish the Farr Institute of Health Informatics Research at UCLPartners, from the Medical Research Council, Arthritis Research UK, British Heart Foundation, Cancer Research UK, Chief Scientist Office, Economic and Social Research Council, Engineering and Physical Sciences Research Council, National Institute for Health Research, National Institute for Social Care and Health Research, and Wellcome Trust (grant MR/K006584/1). SB and AB were supported by grants from the European Research Council (ERC-StG-2012- 309337_AlcoholLifecourse) and the Medical Research Council/Alcohol Research UK (MR/M006638/1). JG was funded by a NIHR doctoral fellowship (DRF-2009-02-50). ADS is supported by a clinical research training fellowship from the Wellcome Trust (0938/30/Z/10/Z). This article presents independent research funded in part by the NIHR. The views expressed are those of the authors and not necessarily those of the NHS, the NIHR, or the Department of Health
Governors and directors: Competing models of corporate governance
Why do we use the term âcorporate governanceâ rather than âcorporate directionâ? Early British joint stock companies were normally managed by a single âgovernorâ. The âcourt of governorsâ or âboard of directorsâ emerged slowly as the ruling body for companies. By the nineteenth century, however, companies were typically run by directors while not-for-profit entities such as hospitals, schools and charitable bodies had governors. The nineteenth century saw steady refinement of the roles of company directors, often in response to corporate scandals, with a gradual change from the notion of the director as a ârepresentative shareholderâ to the directors being seen collectively as ârepresentatives of the shareholdersâ. Governors in not-for-profit entities, however, were regarded as having broader responsibilities. The term âgovernanceâ itself suggests that corporate boards should be studied as âpoliticalâ entities rather than merely through economic lenses such as agency theory
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