Reproductive biology and condition factor of the African catfish, Clarias gariepinus (Burchell 1822) in Lake Koka, Ethiopia

The study was conducted to investigate the breeding season, sex ratio, size at maturation, fecundity and condition factor of African catfish Clarias gariepinus in Lake Koka. A total of 754 fish specimens were collected from October 2020 to August 2021. Total length and weight were measured and sexes were identified and gonad maturity levels were recorded. The sex ratio of African catfish in different size classes was similar to that of a hypothetical 1:1 ratio. The length at first maturity (L50) was 51.8 cm in total length. The mean Fulton’s condition factor of female African catfish ranged from 0.65 ± 0.05 – 0.71 ± 0.14, while the males ranged from 0.62 ± 0.04 – 0.7 ± 0.11. The Fulton’s condition factor was significantly higher in August for females than in the rest of the sampling months. The highest ripe gonads were recorded in the rainy season and the lowest was in the dry season, with the percentage of ripe gonads being 27.5%, while the males showed similarly extended maturity patterns among seasons. Fisheries management for African catfish should therefore take into account the estimated L50 value and the main breeding season to sustain the fishery and benefits the fishermen

Exploring the Variation in Grain and Straw Yield and Straw Quality Traits of Improved Varieties of Tef [Eragrostis tef (Zucc.) Trotter]

Tef [Eragrostis tef (Zucc.) Trotter] is an indigenous Ethiopian cereal providing healthy and nutritious diets for people and a palatable straw for livestock. Thirty-five released varieties and a local check were grown at two locations over two years in Ethiopia to investigate the variation in grain and straw yield and straw quality traits of tef. The investigated traits were grain yield (GY), straw yield (STY), crude protein (CP), in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), acid detergent fiber (ADF), neutral detergent fiber (NDF) and acid detergent lignin (ADL). GY, CP, IVOMD and ME were significantly (P < 0.01) affected by the variety of tef, the cultivation environment and their interactions. The performances of the GY, CP, IVOMD and ME traits were higher (P < 0.01) at mid-altitude, in Debre Zeit, compared to the high-altitude site, Holetta, and in 2016 than 2015. Similarly, the associations observed between GY and STY, among CP, ME and IVOMD, and among NDF, ADF and ADL were significant (P<0.01) and positive. Conversely, the association between yield and quality traits was significant and negative. The present study also identified four groups of varieties based on their grain and straw yield and straw quality traits, with some varieties performing above the mean for both yield and quality traits. Hence, varieties like Melko which possess better grain and straw yield and quality traits could be promoted for immediate utilization as a dual-purpose variety or as a parental line in changing the existing tef breeding strategy that focuses on grain yield improvement alone

Spatiotemporal distribution and bionomics of Anopheles stephensi in different eco-epidemiological settings in Ethiopia

Background: Malaria is a major public health concern in Ethiopia, and its incidence could worsen with the spread of the invasive mosquito species Anopheles stephensi in the country. This study aimed to provide updates on the distribution of An. stephensi and likely household exposure in Ethiopia. Methods: Entomological surveillance was performed in 26 urban settings in Ethiopia from 2021 to 2023. A kilometer-by-kilometer quadrant was established per town, and approximately 20 structures per quadrant were surveyed every 3 months. Additional extensive sampling was conducted in 50 randomly selected structures in four urban centers in 2022 and 2023 to assess households’ exposure to An. stephensi. Prokopack aspirators and CDC light traps were used to collect adult mosquitoes, and standard dippers were used to collect immature stages. The collected mosquitoes were identified to species level by morphological keys and molecular methods. PCR assays were used to assess Plasmodium infection and mosquito blood meal source. Results: Catches of adult An. stephensi were generally low (mean: 0.15 per trap), with eight positive sites among the 26 surveyed. This mosquito species was reported for the first time in Assosa, western Ethiopia. Anopheles stephensi was the predominant species in four of the eight positive sites, accounting for 75–100% relative abundance of the adult Anopheles catches. Household-level exposure, defined as the percentage of households with a peridomestic presence of An. stephensi, ranged from 18% in Metehara to 30% in Danan. Anopheles arabiensis was the predominant species in 20 of the 26 sites, accounting for 42.9–100% of the Anopheles catches. Bovine blood index, ovine blood index and human blood index values were 69.2%, 32.3% and 24.6%, respectively, for An. stephensi, and 65.4%, 46.7% and 35.8%, respectively, for An. arabiensis. None of the 197 An. stephensi mosquitoes assayed tested positive for Plasmodium sporozoite, while of the 1434 An. arabiensis mosquitoes assayed, 62 were positive for Plasmodium (10 for P. falciparum and 52 for P. vivax). Conclusions: This study shows that the geographical range of An. stephensi has expanded to western Ethiopia. Strongly zoophagic behavior coupled with low adult catches might explain the absence of Plasmodium infection. The level of household exposure to An. stephensi in this study varied across positive sites. Further research is needed to better understand the bionomics and contribution of An. stephensi to malaria transmission. Graphical Abstract

Knowledge and associated factors towards diabetes mellitus among adult non-diabetic community members of Gondar city, Ethiopia 2019.

IntroductionDiabetes mellitus is a metabolic disorder resulting from either loss of insulin producing cells, insufficient insulin action, or both. Knowledge can play an important role in preventing diabetes mellitus and its complications. There is limited information regarding knowledge and related factors regarding diabetes mellitus among non-diabetic adult community members in the study area. Therefore, the current study aimed to determine knowledge and associated factors towards diabetes mellitus among non-diabetes community members of Gondar city.MethodsCommunity-based cross-sectional study was conducted on non-diabetic community members from July 1-29, 2019 in Gondar city. The participants were selected randomly from each households. A structured questionnaire was used to collect the data. EpiData version 3.1 was used for data entry and SPSS version 20 was used for data processing and analysis. Descriptive statistics were calculated for most variables. Multivariable logistic regression was used to identify the associated factors. A variable was considered significantly associated at p-valueResultA total of 633 study subjects participated in this study with a mean age of 36.12 (± 12.87) years. Of these study participants, 572 had awareness about diabetes mellitus and 51.4% (95% CI: 47.4%, 55.8%) had good knowledge. Being male [Adjusted odds ratio = 1.62 (95% CI: 1.05, 2.48)], monthly income of 3000-5000 birr [Adjusted odds ratio = 1.88 (95% CI: 1.03, 3.41)], monthly income of ≥5001 birr [Adjusted odds ratio = 2.37 (95% CI: 1.17, 4.78)], previous training on diabetes mellitus [Adjusted odds ratio = 4.37 (95% CI; 3.04, 7.37)], being grade 9-12 [Adjusted odds ratio = 3.1 (95% CI: 1.09, 8.66)], having college and above educational qualification [Adjusted odds ratio = 3.70 (95% CI: 1.26, 10.85)] were significantly associated with good knowledge towards diabetes mellitus.ConclusionThe level of knowledge regarding diabetes mellitus was low among study participants which indicates a need for health education intervention. Previous training on diabetes mellitus, educational status and average monthly income and being male were the factors associated with good knowledge of participants about diabetes mellitus

Antimalarial compounds from Kniphofia foliosa roots.

During the course of screening Ethiopian medicinal plants for their antimalarial properties, it was found that the dichloromethane extract of the roots of Kniphofia foliosa Hochst. (Asphodelaceae), which have long been used in the traditional medicine of Ethiopia for the treatment of abdominal cramps and wound healing, displayed strong in vitro antiplasmodial activity against the chloroquine-sensitive 3D7 strain of Plasmodium falciparum with an ED50 value of 3.8 microg/mL and weak cytotoxic activity against KB cells with an ED50 value of 35.2 microg/mL. Five compounds were isolated from the roots and evaluated for their in vitro antimalarial activity. Among the compounds tested, 10-(chrysophanol-7'-yl)-10-(xi)-hydroxychrysopanol-9-anthrone and chryslandicin, showed a high inhibition of the growth of the malaria parasite, P. falciparum with ED50 values of 0.260 and 0.537 microg/mL, respectively, while the naphthalene derivative, 2-acetyl-1-hydroxy-8-methoxy-3-methylnaphthalene, exhibited a less significant antimalarial activity with an ED50 value of 15.4 microg/mL. To compare the effect on the parasite with toxicity to mammalian cells, the cytotoxic activities of the isolated compounds against the KB cell line were evaluated and 10-(chrysophanol-7'-yl)-10-(xi)-hydroxychrysopanol-9-anthrone and chryslandicin displayed very low toxicity with ED50 values of 104 and 90 microg/mL, respectively. This is the first report of the inhibition of the growth of P. falciparum by anthraquinone-anthrone dimers and establishes them as a new class of potential antimalarial compounds with very little host cell toxicity

Prevalence of Depression and Associated Factors among Diabetes Patients in East Shewa, Ethiopia: Bayesian Approach

Background. Depression is one of the most pressing public health problems and also highly prevalent comorbid condition among diabetes mellitus (DM) patients. Depression may impact lifestyle decisions and ability to poorly perform tasks which are risk factors for DM. For reducing the impact of depression among DM patients in developing countries, it is crucial to identify and assess associated risk factors of depression among DM patients, thereby designing effective management techniques. In line with this, the current study applies the Bayesian framework, which pools prior information and current data, to find factors associated with depression among DM patients. Methods. A hospital-based cross-sectional study was conducted at Adama Hospital and Medical College (AHMC) from March to April 2019. Data was entered into the Epi-data 3.1 then exported to the R software 3.4.4. Bayesian logistic regression models were fitted to the data using the Markov chain Monte Carlo (MCMC) algorithm. Estimates of model parameters including adjusted odds ratio (AOR) with 95% credible intervals (CI) were calculated. Results. A total of 359 adults with DM were included in the analysis. The prevalence of depression among diabetic patients was 9.22% (95% CI: 6.4% to 12.7%). Higher fasting blood sugar level (AOR=−1.012; HPD CI: (1.0020, 1.025)), having diabetic complication (AOR=0.1876; HPD CI: (0.0214, 0.671)), history of hospital admission (AOR=0.2865; HPD CI: (0.0711, 0.7318)), low medication adherence (AOR=29.29; HPD CI: (3.383, 92.26)), and taking both insulin and oral antidiabetic medication (AOR=24.46; HPD CI: (15.20, 49.37) were significantly and strongly associated with depression among DM patients. Conclusions. Prevalence of depression among diabetes patients in the catchment area of Adama Hospital, Ethiopia, was found to be very low. Higher fasting blood sugar level, diabetic complication, history of hospital admission, low medication adherence, and taking both insulin and oral antidiabetic medication were found to be strong predictors of prevalence of depression among DM patients. Based on the findings, we recommend that integrating screening and treating of depression, early detection and management of diabetic complication, and giving counseling to improve medication adherence is an effective approach for lowering the impact of depression on DM patients

Exploring the variation in grain and straw yield and straw quality traits of improved varieties of Tef [Eragrostis tef (Zucc.) Trotter]

Tef [Eragrostis tef (Zucc.) Trotter] is an indigenous Ethiopian cereal providing healthy and nutritious diets for people and a palatable straw for livestock. Thirty-five released varieties and a local check were grown at two locations over two years in Ethiopia to investigate the variation in grain and straw yield and straw quality traits of tef. The investigated traits were grain yield (GY), straw yield (STY), crude protein (CP), in vitro organic matter digestibility (IVOMD), metabolizable energy (ME), acid detergent fiber (ADF), neutral detergent fiber (NDF) and acid detergent lignin (ADL). GY, CP, IVOMD and ME were significantly (P < 0.01) affected by the variety of tef, the cultivation environment and their interactions. The performances of the GY, CP, IVOMD and ME traits were higher (P < 0.01) at mid-altitude, in Debre Zeit, compared to the high-altitude site, Holetta, and in 2016 than 2015. Similarly, the associations observed between GY and STY, among CP, ME and IVOMD, and among NDF, ADF and ADL were significant (P<0.01) and positive. Conversely, the association between yield and quality traits was significant and negative. The present study also identified four groups of varieties based on their grain and straw yield and straw quality traits, with some varieties performing above the mean for both yield and quality traits. Hence, varieties like Melko which possess better grain and straw yield and quality traits could be promoted for immediate utilization as a dual-purpose variety or as a parental line in changing the existing tef breeding strategy that focuses on grain yield improvement alone

Hypertriglyceridemia and Other Plasma Lipid Profile Abnormalities among People Living with Diabetes Mellitus in Ethiopia: A Systematic Review and Meta-Analysis

Background. Dyslipidemia is one of the leading causes of cardiovascular complications in diabetes mellitus (DM) patients. Though it is a major public health problem in Ethiopia, there is no a nation-wide study to determine dyslipidemia among DM patients yet. Therefore, this systematic review and meta-analysis intended to estimate the prevalence of hypertriglyceridemia and other plasma lipid abnormalities among people living with DM in Ethiopia. Methods. We systematically searched PubMed, Google Scholar, African Journals Online, Hinari, and direct Google. Studies conducted until May 9, 2020, that reports the prevalence of dyslipidemia among people living with DM were included. The DerSimonian and Laird random-effects model was used to determine the pooled prevalence of lipid profile abnormalities. Heterogeneity was checked using the I2 statistic, whereas publication bias was tested by funnel plot and Egger’s test. Besides, subgroup and sensitivity analyses were performed. Results. We used 18 primary studies, including 4961 participants living with DM, which met the eligibility criteria for the meta-analysis of hypertriglyceridemia. The estimate of hypertriglyceridemia (≥150 mg/dl) was 48.15% (95% CI: 38.15-58.15, I2=98.4%) after performing the main meta-analysis using the random-effects model. The subgroup analysis showed a higher pooled estimate of hypertriglyceridemia among T2DM (57.80% (95% CI: 50.50-65.10), I2=92.5%), studies that used probability sampling technique (59.09% (95% CI: 43.58-74.59), I2=98.6%, p<0.001), and studies from primary data sources (51.43% (95% CI: 40.72-62.13), I2=98.0%, p<0.001). Moreover, the estimated pooled prevalence of the total plasma cholesterol (TC≥200 mg/dl) was 34.08% (95% CI: 28.41-39.75, I2=92.4%), LDL−C≥100 mg/dl was 41.13% (95% CI: 27.15-55.11, I2 = 98.8%), and HDL≤40 mg/dl for men and≤50 mg/dl for women was 44.36% (95% CI: 31.82-56.90, I2=98.8%). Conclusions. The pooled prevalence of hypertriglyceridemia and other lipid abnormalities among DM patients was relatively high in Ethiopia. It strongly suggests the need to give maximal attention to the adherence of DM management to reduce the circulatory lipid profile abnormalities and subsequent complications. Prospero Registration. CRD42020182291