A Causal Analysis of Women Engaging in Commercial Sex

Female sex workers (FSWs) are women who engage in sexual behavior with others for monetary compensation. The FSW community has comparable demographic characteristics to the overall population, with a focus on youth, low education, and high mobility. Some women engage in sex trafficking on purpose, not to earn money, but to violate social norms. Feminists who grow up in broken or dysfunctional homes have their familial support networks disturbed. Disruptions to the financial support network include forced departures due to financial restrictions, unemployment, and so on. Women’s decisions to enter the sector are significantly influenced by peer behavior. Sexual assault may have a detrimental effect on an individual’s viewpoint, self-awareness, and emotional capacity. While the majority of FSWs are motivated by economic considerations, a sizable percentage is driven to prostitution. Other women forced into the sex trade have great things to say about it, and when given the opportunity to leave, some opt to stay.

Thanos: High-performance CPU-GPU based balanced graph partitioning using cross-decomposition

As graphs become larger and more complex, it is becoming nearly impossible to process them without graph partitioning. Graph partitioning creates many subgraphs which can be processed in parallel thus delivering high-speed computation results. However, graph partitioning is a difficult task. In this work, we introduce Thanos, a fast graph partitioning tool which uses the cross-decomposition algorithm that iteratively partitions a graph. It also produces balanced loads of partitions. The algorithm is well suited for parallel GPU programming which leads to fast and high-quality graph partitioning solutions. Experimental results show that we have achieved a 30x speedup and 35% better edge cut reduction compared to the CPU version of METIS on average

The ancient phosphatidylinositol 3-kinase signaling system is a master regulator of energy and carbon metabolism in algae

Algae undergo a complete metabolic transformation under stress by arresting cell growth, inducing autophagy and hyperaccumulating biofuel precursors such as triacylglycerols and starch. However, the regulatory mechanisms behind this stress-induced transformation are still unclear. Here, we use biochemical, mutational, and “omics” approaches to demonstrate that PI3K signaling mediates the homeostasis of energy molecules and influences carbon metabolism in algae. In Chlamydomonas reinhardtii, the inhibition and knockdown (KD) of algal class III PI3K led to significantly decreased cell growth, altered cell morphology, and higher lipid and starch contents. Lipid profiling of wild-type and PI3K KD lines showed significantly reduced membrane lipid breakdown under nitrogen starvation (-N) in the KD. RNA-seq and network analyses showed that under -N conditions, the KD line carried out lipogenesis rather than lipid hydrolysis by initiating de novo fatty acid biosynthesis, which was supported by tricarboxylic acid cycle down-regulation and via acetyl-CoA synthesis from glycolysis. Remarkably, autophagic responses did not have primacy over inositide signaling in algae, unlike in mammals and vascular plants. The mutant displayed a fundamental shift in intracellular energy flux, analogous to that in tumor cells. The high free fatty acid levels and reduced mitochondrial ATP generation led to decreased cell viability. These results indicate that the PI3K signal transduction pathway is the metabolic gatekeeper restraining biofuel yields, thus maintaining fitness and viability under stress in algae. This study demonstrates the existence of homeostasis between starch and lipid synthesis controlled by lipid signaling in algae and expands our understanding of such processes, with biotechnological and evolutionary implications.Ministry of Science, ICT and Future Planning 2015M3A6A2065697Ministry of Oceans and Fisheries 2015018

Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins

<p>Abstract</p> <p>Background</p> <p><it>Escherichia coli </it>has been most widely used for the production of valuable recombinant proteins. However, over-production of heterologous proteins in <it>E. coli </it>frequently leads to their misfolding and aggregation yielding inclusion bodies. Previous attempts to refold the inclusion bodies into bioactive forms usually result in poor recovery and account for the major cost in industrial production of desired proteins from recombinant <it>E. coli</it>. Here, we describe the successful use of the immobilized folding machineries for <it>in vitro </it>refolding with the examples of high yield refolding of a ribonuclease A (RNase A) and cyclohexanone monooxygenase (CHMO).</p> <p>Results</p> <p>We have generated refolding-facilitating media immobilized with three folding machineries, mini-chaperone (a monomeric apical domain consisting of residues 191–345 of GroEL) and two foldases (DsbA and human peptidyl-prolyl <it>cis-trans </it>isomerase) by mimicking oxidative refolding chromatography. For efficient and simple purification and immobilization simultaneously, folding machineries were fused with the positively-charged consecutive 10-arginine tag at their C-terminal. The immobilized folding machineries were fully functional when assayed in a batch mode. When the refolding-facilitating matrices were applied to the refolding of denatured and reduced RNase A and CHMO, both of which contain many cysteine and proline residues, RNase A and CHMO were recovered in 73% and 53% yield of soluble protein with full enzyme activity, respectively.</p> <p>Conclusion</p> <p>The refolding-facilitating media presented here could be a cost-efficient platform and should be applicable to refold a wide range of <it>E. coli </it>inclusion bodies in high yield with biological function.</p

The Notice-and-Choice Privacy Gamble: Game Theory, Consumer Agency, and Implications for GDPR

This article provides a theoretical basis for why the notice-and-choice model for protecting consumer information privacy might still be considered a viable policy approach despite evidence that privacy notices are often ignored, difficult to read, and misunderstood by consumers. Drawing from several well-known game-theoretic models that map closely to an online consumer’s notice-and-choice context, we outline a rational choice model for consumer online privacy and discuss its relevance for the EU’s General Data Protection Regulations [GDPR]. We argue that an online consumer’s notice-and-choice privacy gamble is a reasonable bet when constrained by competition and the presence of meaningful regulation