Contamination analysis of Arctic ice samples as planetary field analogs and implications for future life-detection missions to Europa and Enceladus

Missions to detect extraterrestrial life are being designed to visit Europa and Enceladus in the next decades. The contact between the mission payload and the habitable subsurface of these satellites involves significant risk of forward contamination. The standardization of protocols to decontaminate ice cores from planetary field analogs of icy moons, and monitor the contamination in downstream analysis, has a direct application for developing clean approaches crucial to life detection missions in these satellites. Here we developed a comprehensive protocol that can be used to monitor and minimize the contamination of Arctic ice cores in processing and downstream analysis. We physically removed the exterior layers of ice cores to minimize bioburden from sampling. To monitor contamination, we constructed artificial controls and applied culture-dependent and culture-independent techniques such as 16S rRNA amplicon sequencing. We identified 13 bacterial contaminants, including a radioresistant species. This protocol decreases the contamination risk, provides quantitative and qualitative information about contamination agents, and allows validation of the results obtained. This study highlights the importance of decreasing and evaluating prokaryotic contamination in the processing of polar ice cores, including in their use as analogs of Europa and Enceladus.info:eu-repo/semantics/publishedVersio

Blade debitage in Southern Brazil: We have cores! [Debitagem laminar no Sul do Brasil: Habemus nucleos!]

Durante a pré-história, a debitagem laminar é um modo de produção característico de determinados períodos e regiões. No Brasil, no momento, foi reconhecida em três sítios arqueológicos situados no alto vale do rio Uruguai (SC/RS), em contextos sedimentares que datam do Holoceno inicial. Até hoje, somente os produtos dessa debitagem, as lâminas, tinham sido encontrados. A falta dos núcleos impedia um entendimento completo do processo de lascamento desses suportes. Graças à retomada das pesquisas na região desde 2013, foi possível coletar núcleos associados a essa produção laminar. Nesse artigo, descrevemos cinco desses núcleos. Suas análises demonstram que essa debitagem laminar corresponde a um único conceito. A estrutura volumétrica do núcleo que resulta desse conceito respeita as seguintes normas: 1) Os núcleos apresentam duas superfícies: um dorso plano e uma superfície de debitagem convexa mas relativamente achatada. Em uma extremidade, uma pequena superfície plana serviu de plano de percussão; 2) A debitagem começa por uma fase de inicialização durante a qual o plano de percussão é produzido por uma grande retirada. Em função das propriedades naturais do volume inicial, o dorso do núcleo é obtido, seja durante a seleção do bloco, seja por uma preparação por grandes retiradas transversais. Quando existem ainda, os negativos de retiradas da inicialização da superfície de debitagem indicam um método centrípeto; 3) As lâminas são sempre produzidas por um método unidirecional paralelo. A variabilidade desta debitagem é principalmente relacionada à técnica de lascamento: a percussão com pedra e a percussão com percutor orgânico foram utilizadas para a obtenção das lâminas. As informações tecnológicas fornecidas pelos núcleos aqui analisados são coerentes com essas observações oriundas da análise das lâminas. Com esses dois estudos consegue-se atingir um conhecimento completo desse sistema de produção do alto vale do rio Uruguai durante o Holoceno inicial, a única debitagem laminar documentada até hoje no Brasil.Blade debitage is a characteristic production pattern of specific periods and regions during Prehistory. In South America, it is well documented in Argentina, in contexts dating back to early Holocene. In Brazil, it was unknown until 2006, when it was found in three archaeological sites (ACH-LP-01, ACH-LP-03, ALP-AA-03) in the Foz do Chapecó area, upper Uruguay River, South Brazil, in layers from early Holocene. It was associated there with a great variety of other production systems: bifacial shaping of projectile points and large tools, unipolar debitage of large flakes, bipolar debitage of tiny flakes. Silicified sandstone, chalcedony and hyaline quartz are the main used raw materials. To this day, only the products of this debitage, the blades, had been discovered. Cores were lacking, which prevented a complete understanding of the flaking process. Due to the resumption of research in the region from 2013, it was possible to find those cores during surveys in the two following archaeological sites: ACH-LP-07 and RS-URG-01, located on both sides of the Uruguay river, near the mouth of the Chapecó river. We describe in this article five of these cores (4 from ACH-LP-07 and 1 from RS-URG-01). Their analyses demonstrate that this blade debitage complies with only one concept. The core volumetric structure that is the result of this concept displays the following features: 1) Cores have two surfaces: a flat back and a convex but relatively flattened flaking surface, so that they have a general “D” shaped transversal section. At one end, a small surface acts as striking platform during the production of the blades; 2) Debitage begins with a initialization phase of the core during which the striking platform is produced by a large removal. According to the natural properties of the initial volume, the back of the core is obtained either during the selection of the blank, and then it is left in his natural state, or by a preparation by one or more large transversal removals. When present, the scars of the initialization phase of the flaking surface indicate the use of a centripetal method. This preparation is made possible by the acute angle of the peripheral ridge formed by the intersection of the back and the flaking surface ; 3) Blades are always produced by a unidirectional parallel method. Production variability is mainly related to the flaking technique: both internal percussion by stone and marginal percussion by organic percussor were used to get the blades. Technical information provided by the cores are complementary and in accordance with those from the previous analyses of the blades from the same area. With these two studies it was possible to reach a relatively exhaustive understanding of this production system in the upper Uruguay River during early Holocene, the only well documented blade debitage in Brazil known until now. Future research will explore the interactions between this production and the other debitage and shaping methods inside this early Holocene technological system. To do this, we will develop a comprehensive study of all lithic artefacts from the archaeological layers in which blade production was identified. In a wider scale, a comparative approach with Argentinian and Uruguayan prehistoric blade productions will allow to better understand the development of the blade phenomenon in the southern cone of South America

Automated database-guided expert-supervised orientation for immunophenotypic diagnosis and classification of acute leukemia

Precise classification of acute leukemia (AL) is crucial for adequate treatment. EuroFlow has previously designed an AL orientation tube (ALOT) to guide towards the relevant classification panel (T-cell acute lymphoblastic leukemia (T-ALL), B-cell precursor (BCP)-ALL and/or acute myeloid leukemia (AML)) and final diagnosis. Now we built a reference database with 656 typical AL samples (145 T-ALL, 377 BCP-ALL, 134 AML), processed and analyzed via standardized protocols. Using principal component analysis (PCA)-based plots and automated classification algorithms for direct comparison of single-cells from individual patients against the database, another 783 cases were subsequently evaluated. Depending on the database-guided results, patients were categorized as: (i) typical T, B or Myeloid without or; (ii) with a transitional component to another lineage; (iii) atypical; or (iv) mixed-lineage. Using this automated algorithm, in 781/783 cases (99.7%) the right panel was selected, and data comparable to the final WHO-diagnosis was already provided in >93% of cases (85% T-ALL, 97% BCP-ALL, 95% AML and 87% mixed-phenotype AL patients), even without data on the full-characterization panels. Our results show that database-guided analysis facilitates standardized interpretation of ALOT results and allows accurate selection of the relevant classification panels, hence providing a solid basis for designing future WHO AL classifications

Proposta metodologica de analise de material litico préhistorico ou como ver uma pedra lascada como uma Matrioshka

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