721 research outputs found

    Neighborhood Factors that Contribute to Alcohol Use and Loneliness in HIV Positive Patients

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    Background: Neighborhood factors contribute to substance abuse and increased health risk behavior. Alcohol use has adverse consequences as it may interfere with antiretroviral medication adherence. In addition, studies have shown that those who are HIV positive have decreased social network size, limited social support, and social isolation as well as decreased treatment adherence. It is hypothesized that participants with high neighborhood density of alcohol outlets combined with increased feelings of loneliness will be more likely to drink. Methods: Participants included 85 patients from an HIV treatment clinic in Jacksonville. Interviewer-administered measures included the Alcohol Use Disorders Identification Test (AUDIT) and UCLA Loneliness Scale. Geographical Information Systems was used to map participant residential area and surrounding neighborhood factors. This study collected cross-sectional, retrospective data. Multi-linear regression using UCLA scores and geographic alcohol outlets availability were used as predictors of drinking behavior. Results: UCLA scores (β = 0.088, p = .012) and number of alcohol outlets (β = 0.040, p = .028) were significant predictors of AUDIT scores. UCLA scores and number of alcohol outlets accounted for 10.4% (R2 = .104) of variance of AUDIT scores. Conclusion: There was co-occurrence of alcohol use and self-reported loneliness among patients currently in treatment for HIV. There also appears to be a relationship with neighborhood factors, alcohol use, and loneliness but further research is needed

    Sustaining Economic Exploitation of Complex Ecosystems in Computational Models of Coupled Human-Natural Networks

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    Understanding ecological complexity has stymied scientists for decades. Recent elucidation of the famously coined "devious strategies for stability in enduring natural systems" has opened up a new field of computational analyses of complex ecological networks where the nonlinear dynamics of many interacting species can be more realistically mod-eled and understood. Here, we describe the first extension of this field to include coupled human-natural systems. This extension elucidates new strategies for sustaining extraction of biomass (e.g., fish, forests, fiber) from ecosystems that account for ecological complexity and can pursue multiple goals such as maximizing economic profit, employment and carbon sequestration by ecosystems. Our more realistic modeling of ecosystems helps explain why simpler "maxi-mum sustainable yield" bioeconomic models underpinning much natural resource extraction policy leads to less profit, biomass, and biodiversity than predicted by those simple models. Current research directions of this integrated natu-ral and social science include applying artificial intelligence, cloud computing, and multiplayer online games

    Production of latex agglutination reagents for pneumococcal serotyping

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    BACKGROUND: The current ‘gold standard’ for serotyping pneumococci is the Quellung test. This technique is laborious and requires a certain level of training to correctly perform. Commercial pneumococcal latex agglutination serotyping reagents are available, but these are expensive. In-house production of latex agglutination reagents can be a cost-effective alternative to using commercially available reagents. This paper describes a method for the production and quality control (QC) of latex reagents, including problem solving recommendations, for pneumococcal serotyping. RESULTS: Here we describe a method for the production of latex agglutination reagents based on the passive adsorption of antibodies to latex particles. Sixty-five latex agglutination reagents were made using the PneuCarriage Project (PCP) method, of which 35 passed QC. The other 30 reagents failed QC due to auto-agglutination (n=2), no reactivity with target serotypes (n=8) or cross-reactivity with non-target serotypes (n=20). Dilution of antisera resulted in a further 27 reagents passing QC. The remaining three reagents passed QC when prepared without centrifugation and wash steps. Protein estimates indicated that latex reagents that failed QC when prepared using the PCP method passed when made with antiserum containing ≤ 500 μg/ml of protein. Sixty-one nasopharyngeal isolates were serotyped with our in-house latex agglutination reagents, with the results showing complete concordance with the Quellung reaction. CONCLUSIONS: The method described here to produce latex agglutination reagents allows simple and efficient serotyping of pneumococci and may be applicable to latex agglutination reagents for typing or identification of other microorganisms. We recommend diluting antisera or removing centrifugation and wash steps for any latex reagents that fail QC. Our latex reagents are cost-effective, technically undemanding to prepare and remain stable for long periods of time, making them ideal for use in low-income countries

    Exploring the Contribution of Parental Perceptions to Childhood Anxiety

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    Parental rearing practices such as over-involvement are associated with childhood anxiety; however, little is known about the contribution of parental perceptions to child anxiety. This study explores the relationship between maternal and paternal perceptions of parenting and childhood anxiety. The perceived rearing behaviors and parental sense of competence (i.e., satisfaction and efficacy) of the parents of anxious children (n = 59) were compared with those of a non-clinical control sample (n = 44). In line with the findings from the literature that addresses externalizing disorders, parental sense of competence was significantly associated with childhood outcomes. Logistical regression suggested that paternal efficacy beliefs, acceptance, and maternal satisfaction were associated with an absence of clinical anxiety and lower levels of anxiety symptoms in children. Parental perceptions may thus provide an important area for understanding childhood anxiety
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