Journalism in a state of flux

The move to a networked media environment presents a range of challenges for journalistic roles, norms and daily practices. This article employs actor network theory to investigate how different actors negotiate and ultimately shape the manner in which the internet and related digital technologies are embedded in the newsroom. Findings suggest that professional culture - articulated in skills, ideas and practices - acts as a network that weakens the potential impact of technology towards innovation and audience-oriented models of journalism. The results point to the conclusion that the internet and related tools are seen as empowering journalists to do their (traditional) jobs better instead of moving on to the next stage built around a stronger commitment to capitalize on the growing sociotechnical potential

A novel patient-derived tumorgraft model with TRAF1-ALK anaplastic large-cell lymphoma translocation

Although anaplastic large-cell lymphomas (ALCL) carrying anaplastic lymphoma kinase (ALK) have a relatively good prognosis, aggressive forms exist. We have identified a novel translocation, causing the fusion of the TRAF1 and ALK genes, in one patient who presented with a leukemic ALK+ ALCL (ALCL-11). To uncover the mechanisms leading to high-grade ALCL, we developed a human patient-derived tumorgraft (hPDT) line. Molecular characterization of primary and PDT cells demonstrated the activation of ALK and nuclear factor kB (NFkB) pathways. Genomic studies of ALCL-11 showed the TP53 loss and the in vivo subclonal expansion of lymphoma cells, lacking PRDM1/Blimp1 and carrying c-MYC gene amplification. The treatment with proteasome inhibitors of TRAF1-ALK cells led to the downregulation of p50/p52 and lymphoma growth inhibition. Moreover, a NFkB gene set classifier stratified ALCL in distinct subsets with different clinical outcome. Although a selective ALK inhibitor (CEP28122) resulted in a significant clinical response of hPDT mice, nevertheless the disease could not be eradicated. These data indicate that the activation of NFkB signaling contributes to the neoplastic phenotype of TRAF1-ALK ALCL. ALCL hPDTs are invaluable tools to validate the role of druggable molecules, predict therapeutic responses and implement patient specific therapies

Bcl-2 and Ca2+ homeostasis in the endoplasmic reticulum

none2Impact factor: 8.254 - 20th of 263 in BIOCHEMISTRY & MOLECULAR BIOLOGY categorynoneP. PINTON; R. RIZZUTOP., Pinton; Rizzuto, Rosari