Response of lightning NOx emissions and ozone production to climate change: Insights from the Atmospheric Chemistry and Climate Model Intercomparison Project

Results from an ensemble of models are used to investigate the response of lightning nitrogen oxide emissions to climate change and the consequent impacts on ozone production. Most models generate lightning using a parameterization based on cloud top height. With this approach and a present-day global emission of 5 TgN, we estimate a linear response with respect to changes in global surface temperature of +0.44 ± 0.05 TgN K−1. However, two models using alternative approaches give +0.14 and −0.55 TgN K−1 suggesting that the simulated response is highly dependent on lightning parameterization. Lightning NOx is found to have an ozone production efficiency of 6.5 ± 4.7 times that of surface NOx sources. This wide range of efficiencies across models is partly due to the assumed vertical distribution of the lightning source and partly to the treatment of nonmethane volatile organic compound (NMVOC) chemistry. Careful consideration of the vertical distribution of emissions is needed, given its large influence on ozone production

The effect of westerlies on East African rainfall and the associated role of tropical cyclones and the Madden–Julian Oscillation

Variability of rainfall in East Africa has major impacts on lives and livelihoods. From floods to droughts, this variability is important on short daily time‐scales to longer decadal time‐scales, as is apparent from the devastating effects of droughts in East Africa over recent decades. Past studies have highlighted the Congo airmass in enhancing East African rainfall. Our detailed analysis of the feature shows that days with a westerly moisture flow, bringing the Congo airmass, enhance rainfall by up to 100% above the daily mean, depending on the time of year. Conversely, there is a suppression of rainfall on days with a strong easterly flow. Days with a westerly moisture flux are in a minority in all seasons but we show that long rains with more westerly days are wetter, and that during the most‐recent decade which has had more frequent droughts (associated with the “Eastern African climate paradox”), there has been few days with such westerlies. We also investigate the influence of the Madden–Julian Oscillation (MJO) and tropical cyclones, and their interaction with the westerly flow. We show that days of westerly moisture flux are more likely during phases 3 and 4 of the MJO and when there are one or more tropical cyclones present. In addition, tropical cyclones are more likely to form during these phases of the MJO, and more likely to be coincident with westerlies when forming to the east of Madagascar. Overall, our analysis brings together many different processes that have been discussed in the literature but not yet considered in complete combination. The results demonstrate the importance of the Congo airmass on daily to climate time‐scales, and in doing so offers useful angles of investigation for future studies into prediction of East African rainfall

Effects of Explicit Convection on Future Projections of Mesoscale Circulations, Rainfall, and Rainfall Extremes over Eastern Africa

Eastern Africa’s fast-growing population is vulnerable to changing rainfall and extremes. Using the first pan-African climate change simulations that explicitly model the rainfall-generating convection, we investigate both the climate change response of key mesoscale drivers of eastern African rainfall, such as sea and lake breezes, and the spatial heterogeneity of rainfall responses. The explicit model shows widespread increases at the end of the century in mean (~40%) and extreme (~50%) rain rates, whereas the sign of changes in rainfall frequency has large spatial heterogeneity (from −50% to over +90%). In comparison, an equivalent parameterized simulation has greater moisture convergence and total rainfall increase over the eastern Congo and less over eastern Africa. The parameterized model also does not capture 1) the large heterogeneity of changes in rain frequency; 2) the widespread and large increases in extreme rainfall, which result from increased rainfall per humidity change; and 3) the response of rainfall to the changing sea breeze, even though the sea-breeze change is captured. Consequently, previous rainfall projections are likely inadequate for informing many climate-sensitive decisions—for example, for infrastructure in coastal cities. We consider the physics revealed here and its implications to be relevant for many other vulnerable tropical regions, especially those with coastal convection

Regional differences in the response of rainfall to convectively coupled Kelvin waves over tropical Africa

The representation of convection remains one of the most important sources of bias in global models and evaluation methods are needed that show that models provide the correct mean state and variability; both for the correct reasons. Here we develop a novel approach for evaluating rainfall variability due to CCKWs in this region. A phase cycle was defined for the CCKW cycle in OLR and used to composite rainfall anomalies. We characterize the observed (TRMM) rainfall response to CCKWs over tropical Africa in April and evaluate the performance of regional climate model (RCM) simulations: a parameterized convection simulation (P25) and the first pan-Africa convection permitting simulation (CP4). TRMM mean rainfall is enhanced and suppressed by CCKW activity and the occurrence of extreme rainfall and dry days is coupled with CCKW activity. Focusing on regional differences, we show for the first time that: there is a dipole between West Africa and the Gulf of Guinea involving onshore/offshore shifts in rainfall; and the transition to enhanced rainfall over west equatorial Africa occurs one phase before the transition over east equatorial Africa. The global model used to drive the RCMs simulated CCKWs with mean amplitudes of 75%-82% of observations. The RCMs simulated coherent responses to the CCKWs and captured the large-scale spatial patterns and phase relationships in rainfall although the simulated rainfall response is weaker than observations and there are regional biases which are bigger away from the equator. P25 produced a closer match to TRMM mean rainfall anomalies than CP4 although the response in dry days was more closely simulated by CP4

Common Mechanism for Interannual and Decadal Variability in the East African Long Rains

The East African long rains constitute the main crop‐growing season in the region. Interannual predictability of this season is low in comparison to the short rains, and recent decadal drying contrasts with climate projections of a wetter future (the “East African climate paradox”). Here, we show that long rains rainfall totals are strongly correlated with 700 hPa zonal winds across the Congo basin and Gulf of Guinea ( urn:x-wiley:grl:media:grl61425:grl61425-math-0001). Westerly anomalies align with more rainfall, with the same mechanism controlling covariability on interannual and decadal time scales. On both time scales wind anomalies are linked to geopotential anomalies over the Sahel and Sahara, and warming there. Rainfall and wind are significantly correlated with the Madden‐Julian Oscillation (MJO) amplitude, and around 18% of the decadal drying can be explained by MJO amplitude variability. This work shows that predictions of East African rainfall across time scales require robust prediction of both zonal winds and MJO activity

Circulation Patterns Associated with Current and Future Rainfall over Ethiopia and South Sudan from a Convection-Permitting Model

Ethiopia and South Sudan contain several population centers and important ecosystems that depend on July–August rainfall. Here we use two models to understand current and future rainfall: the first ever pan-African numerical model of climate change with explicit convection and a parameterized model that resembles a typical regional climate model at 4.5 and 25 km horizontal grid-spacing, respectively. The explicit convection and higher resolution of the first model offer a greatly improved representation of both the frequency and intensity of rainfall, when compared to the parametrized convection model. Furthermore, only this model has success in capturing the east–west propagation of rainfall over the full diurnal cycle. Enhanced low-level westerlies were found for extremely wet days, though this response was weaker in the explicit convection model. The increased orographic detail in the explicit model resulted in the splitting of the low-level Turkana Jet core into smaller cores, and inhibited its penetration far into South Sudan. Some projected changes were found to be independent of model, such as changes in the strength of Somali and Turkana jets, as well as the shifting of Turkana jet core to lower levels. However, the explicit model end-of-century projections showed a larger and clearer decrease in wet days, accompanied by an increase in wet day intensity and extreme rainfall. This study highlights serious limitations of relying solely on simulations which parameterize convection to inform decisions in the region of South Sudan and Ethiopia

African Lightning and its Relation to Rainfall and Climate Change in a Convection‐Permitting Model

Global climate models struggle to simulate both the convection and cloud ice fundamental to lightning formation. We use the first convection‐permitting, future climate simulations for the lightning hot spot of Africa, at the same time utilizing an ice‐based lightning parametrization. Both the model and observations show that lightning over Africa's drier areas, as well as the moist Congo, have more lightning per rainfall than other regions. Contrary to results in the literature, the future projection shows little increase in total lightning (~107 flashes (or 2%) per degree warming). This is a consequence of increased stability reducing the number of lightning days, largely offsetting the increased graupel and updraft velocity driving an increase in lightning per lightning day. The next step is to establish if these results are robust across other models and, if combined with parametrized‐convection models, whether ensemble‐based information on the possible responses of lightning to climate change can be investigated. Plain Language Summary Lightning depends on ascending air in thunderstorms and the collision of cloud ice particles, which charge the thundercloud. Many climate models have too coarse a resolution to reliably capture these processes. We focus on Africa, which has some of the most frequent lightning in the world. We use a model that is much higher resolution than usual, and this allows us to explicitly simulate the deep convection associated with thunderstorms as well as provide more detailed representation of the distribution of cloud ice particles. Our results show that in drier regions, as well as the much wetter Congo, there is relatively more lightning per kilogram of surface rainfall than there is in other parts of the continent. Lightning does increase across the continent under climate change, but by a relatively small amount. This is despite the number of days with lightning decreasing as the lower atmosphere becomes more stable. On days with lightning, there are more lightning flashes because there is an increase in cloud ice and intensity of convection. This study gives much more detailed information about African lightning than previous work. However, it is a single simulation. Future research should look at these results across other climate models

Implications of improved representation of convection for the East Africa water budget using a convection-permitting model

The precipitation and diabatic heating resulting from moist convection make it a key component of the atmospheric water budget in the tropics. With convective parametrisation being a known source of uncertainty in global models, convection-permitting (CP) models are increasingly being used to improve understanding of regional climate. Here, a new 10-year CP simulation is used to study the characteristics of rainfall and atmospheric water budget for East Africa and the Lake Victoria basin. The explicit representation of convection leads to a widespread improvement in the intensities and diurnal cycle of rainfall when compared with a parametrised simulation. Differences in large-scale moisture fluxes lead to a shift in the mean rainfall pattern from the Congo to Lake Victoria basin in the CP simulation - highlighting the important connection between local changes in the representation of convection and larger scale dynamics and rainfall. Stronger lake-land contrasts in buoyancy in the CP model lead to a stronger nocturnal land breeze over Lake Victoria, increasing evaporation and moisture flux convergence (MFC), and likely unrealistically high rainfall. However, for the mountains east of the lake, the CP model produces a diurnal rainfall cycle much more similar to satellite estimates, which is related to differences in the timing of MFC. Results here demonstrate that, whilst care is needed regarding lake forcings, a CP approach offers a more realistic representation of several rainfall characteristics through a more physically-based realisation of the atmospheric dynamics around the complex topography of East Africa

Safety, tumor trafficking and immunogenicity of chimeric antigen receptor (CAR)-T cells specific for TAG-72 in colorectal cancer.

BackgroundT cells engineered to express chimeric antigen receptors (CARs) have established efficacy in the treatment of B-cell malignancies, but their relevance in solid tumors remains undefined. Here we report results of the first human trials of CAR-T cells in the treatment of solid tumors performed in the 1990s.MethodsPatients with metastatic colorectal cancer (CRC) were treated in two phase 1 trials with first-generation retroviral transduced CAR-T cells targeting tumor-associated glycoprotein (TAG)-72 and including a CD3-zeta intracellular signaling domain (CART72 cells). In trial C-9701 and C-9702, CART72 cells were administered in escalating doses up to 1010 total cells; in trial C-9701 CART72 cells were administered by intravenous infusion. In trial C-9702, CART72 cells were administered via direct hepatic artery infusion in patients with colorectal liver metastases. In both trials, a brief course of interferon-alpha (IFN-α) was given with each CART72 infusion to upregulate expression of TAG-72.ResultsFourteen patients were enrolled in C-9701 and nine in C-9702. CART72 manufacturing success rate was 100% with an average transduction efficiency of 38%. Ten patients were treated in CC-9701 and 6 in CC-9702. Symptoms consistent with low-grade, cytokine release syndrome were observed in both trials without clear evidence of on target/off tumor toxicity. Detectable, but mostly short-term (≤14 weeks), persistence of CART72 cells was observed in blood; one patient had CART72 cells detectable at 48 weeks. Trafficking to tumor tissues was confirmed in a tumor biopsy from one of three patients. A subset of patients had 111Indium-labeled CART72 cells injected, and trafficking could be detected to liver, but T cells appeared largely excluded from large metastatic deposits. Tumor biomarkers carcinoembryonic antigen (CEA) and TAG-72 were measured in serum; there was a precipitous decline of TAG-72, but not CEA, in some patients due to induction of an interfering antibody to the TAG-72 binding domain of humanized CC49, reflecting an anti-CAR immune response. No radiologic tumor responses were observed.ConclusionThese findings demonstrate the relative safety of CART72 cells. The limited persistence supports the incorporation of co-stimulatory domains in the CAR design and the use of fully human CAR constructs to mitigate immunogenicity