Glycocomposition of the apocrine interdigital gland secretions in the fallow deer (Dama dama)

The secretions of the tubular interdigital glands were investigated by conventional (Periodic-Acid Schiff, Alcian-Blue at different pH, Low Iron Diamine and High Iron Diamine) and lectin (Con-A, UEA-I, LTA, WGA, GSA-II, GSA-IB4, SBA, PNA, ECA, DBA, MAL-II and SNA) histochemical methods in adult males and females of different age of fallow deer during the breeding season. Sialidase digestion and deacetylation pre-treatment were also employed in conjunction with lectin histochemistry. The glandular epithelium consisted of a single layer of low columnar cells with typical apical protrusions. No substantial differences of the above histochemical staining in relation to sex and age were observed. Conventional histochemical staining revealed that the interdigital glands secreted neutral glycoproteins whereas acidic glycocomponents did not seem to be present. Lectin histochemical technique allowed us to disclose a great heterogeneity of glycoproteins with N- and O-linked oligosaccharides containing alpha-D-Man/alpha-D-Glc, GlcNAc, alpha-Fuc, terminal beta-D-Gal-(1-3)-D-GalNAc, -D-Gal-(1-4)-D-GlcNAc, alpha-Gal and beta-GalNAc residues. beta-GalNAc and disaccharide beta-D-Gal-(1-3)-D-GalNAc were also found as subterminal to sialyl moieties. The lack of sexual and age-related differences in the glucidic content of the glandular secretions seems to indicate that the glycoderivatives may play only an accessory role in the production of odoriferous signals in fallow deer

Towards the Green-Griffiths-Lang conjecture

The Green-Griffiths-Lang conjecture stipulates that for every projective variety X of general type over C, there exists a proper algebraic subvariety of X containing all non constant entire curves f : C $\rightarrow$ X. Using the formalism of directed varieties, we prove here that this assertion holds true in case X satisfies a strong general type condition that is related to a certain jet-semistability property of the tangent bundle TX . We then give a sufficient criterion for the Kobayashi hyperbolicity of an arbitrary directed variety (X,V). This work is dedicated to the memory of Professor Salah Baouendi.Comment: version 2 has been expanded and improved (15 pages

Histochemical detection of the lectin-binding carbohydrates in the zona pellucida during oocyte growth in the wild boar (Sus scrofa scrofa)

The changes that occur in the carbohydrate composition of zona pellucida glycoproteins during oocyte maturation in the wild-boar were studied using periodic-acid Schiff (PAS), High Iron Diamine (HID) and Low Iron Diamine (LID). Lectin staining was performed with a panel of 11 HRP-lectin conjugates combined with neuraminidase digestion and chemical treatments. There were few internal glucidic residues, such as N-acetylglucosamine, in the wild boar zona pellucida but there were many subterminal beta-N-acetylgalactosamine, alpha- and beta-galactose determinants masked by sialic acid. In addition, beta-N-acetylgalactosamine, beta-galactose-(1-3)-N-acetylgalactosamine and beta-galactose-(1-4)-N-acetylglucosamine were detected in the sulphated form in the terminal and/or subterminal position. Some differences in the lectin reactive sites occurred in the zona pellucida, depending on the stage of oocyte maturatio

Welfare Assessment in Shelter Dogs by Using Physiological and Immunological Parameters

This study aimed to evaluate the state of welfare of a group of dogs during the first month after entering the shelter by using dierent stress parameters. Blood and fecal samples were collected from a group of 71 dogs at the time of admission to the shelter. In 46 of these dogs, sampling was repeated after four weeks. Well-recognized welfare biomarkers, such as fecal cortisol and leukocytes, as well as some innovative parameters ( -endorphin and lysozyme) were determined. Uni- and multivariate statistical analyses were used to evaluate their interactions and changes over time. Neutrophils (p < 0.01), lysozyme (p < 0.05), and fecal cortisol (p < 0.05) decreased, while lymphocytes (p < 0.05) increased after four weeks compared to the first days of being in the shelter, suggesting an improvement in the dogs’ welfare over time. A principal component analysis extracted three bipolar components (PCs), explaining 75% of the variance and indicating negative associations between neutrophil and lymphocyte (PC1), lysozyme and -endorphin (PC2), cortisol and lysozyme (PC3). The associations between these variables within each PC also confirmed the intricate relationships between the hypothalamic-pituitary-adrenal (HPA) axis and the immune system as well as the importance of a multiparametric approach in evaluating welfare

Multi-Operator Qualitative Behavioural Assessment for dogs entering the shelter

This study aimed to develop and validate a fast and straightforward welfare assessment system to help shelter staff in decision-making processes. For newly captured dogs entering the shelter, the animal control officer (at capture time) and the veterinary officer (at entrance examination) compiled a form attributing a qualitative score for the overall Stress level and for 5 Descriptors of dog behavioural traits. Furthermore, a tester filled out the same form by subjecting the dog to a test battery in his pen, besides performing behavioural observations. The veterinary officer and the tester repeated the evaluation protocol after four weeks of acclimatisation in the shelter. The analysis evaluated inter-observer and test–retest reliability, internal consistency, and construct validity of qualitative scores. Overall, we collected 258 forms regarding 189 dogs. Principal component (PC) extracted by Descriptors showed a good correlation with the Stress level score for veterinary and tester confirming the internal consistency of these scales while it was low for animal control officer form. Moreover, qualitative evaluations of the veterinarian and the tester showed congruent correlations with behavioural observations supporting their construct validity. Conversely, the scores expressed by the animal control officer were not consistent with quantitative observations. Then, the veterinary officer and tester forms could be validated and further simplified including only Stress level score while the control officer form requires a revision as it does not seem reliable. We did not find agreement between the Stress level scores expressed by animal control officer, veterinary and tester suggesting that the three contexts represent different stress stimuli to which the same dog reacts differently. The point of view of the three evaluators can increase the reliability of the assessment. Static but vigilant behaviours prevailed in newly sheltered dogs but activity, interactions and behavioural diversity increased in the second behavioural observation when the dogs were kept in multiple cages and four weeks of adaptation had passed. Stress level reduced and PCs tended towards the pole of sociability suggesting a reduction of stress after the period of adaptation to the socio-environmental conditions of the shelter. On the other hand, the considerable inter-individual variability in behaviours reflects differences in coping strategies and or in the manifestation of stress. Our simple tool can not replace a multidisciplinary approach to welfare assessment but could help shelter staff for individual management of dogs complying with their different adaptation skills

Rationality in Differential Algebraic Geometry

Parametric Cartan theory of exterior differential systems, and explicit cohomology of projective manifolds reveal united rationality features of differential algebraic geometry.Comment: Abel Symposium 201