5,645 research outputs found
Setup of Modulated Temperature FTIR technique to investigate thermal transitions of polymers
Nell'attività di ricerca dottorale è stata sviluppata una nuova tecnica d’indagine spettroscopica (Modulated Temperature Infrared Spectroscopy, MTFTIR) ed applicata allo studio delle transizioni termiche di polimeri. La tecnica permette lo studio delle variazioni conformazionali e strutturali delle singole macromolecole coinvolte nelle transizioni di fase dei materiali, differenziando inoltre transizioni all’equilibrio termodinamico da stati transienti di non-equilibrio. Ad una prima fase di messa a punto dell’apparato sperimentale e delle metodiche sono susseguiti studi su tre polimeri, il polipropilene isotattico, il polietilentereftalato ed il poli(L-lattide). I
risultati MTFTIR, accoppiati ad analisi calorimetriche, hanno evidenziato dei comportamenti peculiari delle catene polimeriche nelle fasi vetrose, liquide e cristalline dei materiali, non indagabili o difficilmente distinguibili mediante altre tecniche sperimentali
TDP-43 regulates the Microprocessor complex activity during in vitro neuronal differentiation
TDP-43 is an RNA-binding protein implicated in RNA metabolism at several levels. Even if ubiquitously expressed, it is considered as a neuronal activity-responsive factor and a major signature for neurological pathologies, making the comprehension of its activity in the nervous system a very challenging issue. TDP-43 has also been described as an accessory component of the Drosha-DGCR8 microprocessor complex, which is crucially involved in basal and tissue-specific RNA processing events.
In the present study, we exploited in vitro neuronal differentiation systems to investigate the TDP-43 demand for the microprocessor function, focusing on both its canonical microRNA biosynthetic activity and its alternative role as a post-transcriptional regulator of gene expression.
Our findings reveal a novel role for TDP-43 as an essential factor that controls the stability of Drosha protein during neuronal differentiation, thus globally affecting the production of microRNAs. We also demonstrate that TDP-43 is required for the Drosha-mediated regulation of Neurogenin 2, a master gene orchestrating neurogenesis, whereas post-transcriptional control of Dgcr8, another Drosha target, resulted to be TDP-43-independent. These results implicate a previously uncovered contribution of TDP-43 in regulating the abundance and the substrate-specificity of the microprocessor complex and provide new insights onto TDP-43 as a key player in neuronal differentiation
On complex power nonnegative matrices
Power nonnegative matrices are defined as complex matrices having at least
one nonnegative integer power. We exploit the possibility of deriving a Perron
Frobenius-like theory for these matrices, obtaining three main results and
drawing several consequences. We study, in particular, the relationships with
the set of matrices having eventually nonnegative powers, the inverse of M-type
matrices and the set of matrices whose columns (rows) sum up to one
Automated culture of aquatic model organisms: shrimp larvae husbandry for the needs of research and aquaculture
Modern research makes frequent use of animal models, that is, organisms raised and bred experimentally in order to help the understanding of biological and chemical processes affecting organisms or whole environments. The development of flexible, reprogrammable and modular systems that may help the automatic production of 'not-easy-to-keep' species is important for scientific purposes and for such aquaculture needs as the production of alive foods, the culture of small larvae and the test of new culture procedures. For this reason, we planned and built a programmable experimental system adaptable to the culture of various aquatic organisms, at different developmental stages. The system is based on culture cylinders contained into operational tanks connected to water conditioning tanks. A programmable central processor unit controls the operations, that is, water changes, temperature, light irradiance, the opening and closure of valves for the discharge of unused foods, water circulation and filtration and disinfection systems, according to the information received by various probes. Various devices may be set to modify water circulation and water changes to fulfil the needs of given organisms, to avoid damage of delicate structures, improve feeding performances and reduce the risk of movements over the water surface. The results obtained indicate that the system is effective in the production of shrimp larvae, being able to produce Hippolyte inermis post-larvae with low mortality as compared with the standard operation procedures followed by human operators. Therefore, the patented prototype described in the present study is a possible solution to automate and simplify the rearing of small invertebrates in the laboratory and in production plants
Accesso alle fonti scritte di tipo primario per lo studio della storia dell’assistenza infermieristica tramite strumenti web
BACKGROUND:
La ricerca storica è un metodo di ricerca qualitativa il cui scopo è la scoperta di nuove conoscenze tramite l’esame di eventi passati utilizzando documenti, oggetti, immagini, o interviste.
SCOPO:
L’articolo ha lo scopo di fornire indicazioni utili ad individuare presso quali archivi e biblioteche è possibile reperire fonti primarie scritte di interesse infermieristico utilizzando gli strumenti di ricerca web.
METODO:
Studio metodologico. Sono stati selezionati i siti web che con l’ausilio di sistemi di ricerca permettono di individuare documenti storici indicizzati presso archivi o biblioteche nazionali ed internazionali, gestiti sia da soggetti pubblici che privati.
RISULTATI:
Sono stati individuati cinque canali di ricerca. Due dedicati alla ricerca di materiale conservato presso biblioteche, di cui uno relativo ad una rete di biblioteche italiane, ed uno contenente materiale proveniente da biblioteche sia italiane che internazionali. I restanti tre canali sono dedicati alla ricerca in archivio, di cui due relativi al patrimonio archivistico statale ed uno a quello non statale.
DISCUSSIONE:
Gli strumenti di ricerca informatici si rilevano molto utili per individuare quali fonti scritte di tipo primario esistano, e dove siano collocate, offrendo una panoramica del materiale disponibile su un determinato argomento. Permette inoltre di ottimizzare tempi e costi della ricerca, ampliandola in maniera esponenziale.
CONCLUSIONE:
L’accesso alle fonti può variare a seconda dei sistemi di cui dispone il soggetto conservatore. È fondamentale considerare che il solo utilizzo di strumenti informatici non prevede l’inclusione dei documenti conservati in archivi e biblioteche che, ad oggi, non dispongono di motori di ricerca
Wake-up radio-based data forwarding for green wireless networks
This paper presents G-WHARP, for Green Wake-up and HARvesting-based energy-Predictive forwarding, a wake-up radio-based forwarding strategy for wireless networks equipped with energy harvesting capabilities (green wireless networks). Following a learning-based approach, G-WHARP blends energy harvesting and wake-up radio technology to maximize energy efficiency and obtain superior network performance. Nodes autonomously decide on their forwarding availability based on a Markov Decision Process (MDP) that takes into account a variety of energy-related aspects, including the currently available energy and that harvestable in the foreseeable future. Solution of the MDP is provided by a computationally light heuristic based on a simple threshold policy, thus obtaining further computational energy savings. The performance of G-WHARP is evaluated via GreenCastalia simulations, where we accurately model wake-up radios, harvestable energy, and the computational power needed to solve the MDP. Key network and system parameters are varied, including the source of harvestable energy, the network density, wake-up radio data rate and data traffic. We also compare the performance of G-WHARP to that of two state-of-the-art data forwarding strategies, namely GreenRoutes and CTP-WUR. Results show that G-WHARP limits energy expenditures while achieving low end-to-end latency and high packet delivery ratio. Particularly, it consumes up to 34% and 59% less energy than CTP-WUR and GreenRoutes, respectively
Probing Quantum Optical Excitations with Fast Electrons
Probing optical excitations with nanometer resolution is important for
understanding their dynamics and interactions down to the atomic scale.
Electron microscopes currently offer the unparalleled ability of rendering
spatially-resolved electron spectra with combined meV and sub-nm resolution,
while the use of ultrafast optical pulses enables fs temporal resolution and
exposure of the electrons to ultraintense confined optical fields. Here, we
theoretically investigate fundamental aspects of the interaction of fast
electrons with localized optical modes that are made possible by these
advances. We use a quantum-optics description of the optical field to predict
that the resulting electron spectra strongly depend on the statistics of the
sample excitations (bosonic or fermionic) and their population (Fock, coherent,
or thermal), whose autocorrelation functions are directly retrieved from the
ratios of electron gain intensities. We further explore feasible experimental
scenarios to probe the quantum characteristics of the sampled excitations and
their populations.Comment: 13 pages, 6 figures, 56 reference
La figura dell’infermiere nell’Italia pre unitaria attraverso l’analisi comparata di regolamenti ospedalieri. Un protocollo di ricerca
Conflict exposure and labour market outcomes. Evidence from longitudinal data for the Gaza Strip
This paper documents the effect of variations in the individual-level intensity of conflict exposure on various labour market outcomes for Palestinians living in the Gaza Strip. Combining individual-level longitudinal employment data and geolocalised information on conflict-related events, we show that an increase in conflict exposure of the individual, while it does not affect the employment status on average, it has a heterogeneous impact on job transitions depending on the worker being employed in the private or the public sector. We also find that, for those in the private sector, higher conflict exposure reduces the labour income and the number of hours worked. For those in the public sector, the effect of conflict is instead null on both the labour income and the number of hours worked and it is positive on wages. Finally, we provide suggestive evidence that these results are explained by the combination of two mechanisms, namely the conflict-induced change in the health conditions of the workers (which affects the labour supply) and in the level of the local economic activity (which affects the labour demand)
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