73 research outputs found
Radiation sterilisation of cultured human brain tumour cells for clinical immune tumour therapy
Paneth cell - rich regions separated by a cluster of Lgr5+ cells initiate crypt fission in the intestinal stem cell niche
The crypts of the intestinal epithelium house the stem cells that ensure the continual renewal of the epithelial cells that line the intestinal tract. Crypt number increases by a process called crypt fission, the division of a single crypt into two daughter crypts. Fission drives normal tissue growth and maintenance. Correspondingly, it becomes less frequent in adulthood. Importantly, fission is reactivated to drive adenoma growth. The mechanisms governing fission are poorly understood. However, only by knowing how normal fission operates can cancer-associated changes be elucidated. We studied normal fission in tissue in three dimensions using high-resolution imaging and used intestinal organoids to identify underlying mechanisms. We discovered that both the number and relative position of Paneth cells and Lgr5+ cells are important for fission. Furthermore, the higher stiffness and increased adhesion of Paneth cells are involved in determining the site of fission. Formation of a cluster of Lgr5+ cells between at least two Paneth-cell-rich domains establishes the site for the upward invagination that initiates fission
Atuação do enfermeiro na atenção domiciliar: uma revisão integrativa da literatura
Studies on the duration of dna-synthesis and mitosis in irradiated and regenerating epidermis cells in mice, by means of tritium-labelled thymidine.
Studies on cell population kinetics of x-irradiated and shielded mouse epidermis by autoradiography after administration of tritated thy- midine.
Observations by biochemical analysis and autoradiography on labelled deoxyribonucleic acid in the normal and regenerating liver of mice.
Studies on the incorporation of tritiated thymidine in desoxyribonu- cleic acid in mouse tissues and on its radiation effects.
Effects of local roentgen irradiation on the rate of endogenous dehydrogenase activity in the epidermis of hairless mice studies by means of a tetrazolium-reduction method.
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