On the application of surface enhanced Raman scattering to study the interaction of DsRed fluorescent proteins with silver nanoparticles embedded in thin silica layers

The interaction of proteins with silver nanoparticles (AgNPs) is of primary importance to uncover silver antimicrobial efficiency and environmental hazard. This interaction can affect silver reactivity, bioavailability and, eventually, silver toxicity towards the environmental media. Detection of the interaction of DsRed fluorescent proteins with AgNPs embedded in thin silica layers is demonstrated using surface enhanced Raman spectroscopy (SERS), but deep analyses require the design and elaboration of dedicated plasmonic substrates giving a high enhancement factor

Physico-chemical characterization of the interaction of red fluorescent protein — DsRed with silica layers

The Discosoma Recombinant Red Fluorescent (DsRed) protein is the latest member of the family of fluorescent proteins. It holds great promise for applications in biotechnology and cell biology. However, before being used for rational engineering, knowledge on the underlying mechanisms relating the DsRed structural stability and adsorption properties on solid surfaces is highly demanded. The physico-chemical analysis performed in this study reveals that the interaction of DsRed with SiO2 surfaces does not lead to protein denaturation. The secondary structure of DsRed is preserved after adsorption and dehydration. The measured contact angles of sessile droplets with different DsRed concentrations determine the interaction as hydrophilic one. The photoluminescence emission of dehydrated DsRed droplets is found to be slightly red-shifted, peaking at 590 nm

ANALYSE DU FONCTIONNEMENT D'UN REACTEUR DE DEPOT D'OXYDE DE SILICIUM ASSISTE PAR PLASMA ET CARACTERISATION DES FILMS ELABORES

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Spéficité structurale et propriété d'oxyde de silicium déposé par procédé plasma

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Elaboration de dispositifs MOS contenant des nanocristaux de silicium obtenus par PECVD pulsé

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Modélisation électrique des décharges RF dans des mélanges N2O/He pour applications aux dépôts PECVD utilisés en micro-électronique

TOULOUSE3-BU Sciences (315552104) / SudocSudocFranceF

Physicochemical and structural properties of nanocomposite SiCxOyHz films with embedded silver nanoparticles

International audienc

Microstructural and optical characterization of silicon nitride alloy thin films aiming at an optimization of the matrix properties for tandem cells

International audienc

Microstructural and optical characterization of silicon nitride alloy thin films aiming at an optimization of the matrix properties for tandem cells

International audienc

Microstructural and optical characterization of silicon nitride alloy thin films aiming at an optimization of the matrix properties for tandem cells

International audienc