10 research outputs found
SH-SY5Y Hücrelerinde 6-OHDA İle İndüklenen Toksisiteye Karşı Mikroalg Özütlerinin Nörokoruyucu Etkilerinin Değerlendirilmesi
Ipbs-retrotransposons-based genetic diversity and relationship among wild annual Cicer species
Lack of requisite genetic variation in cultivated
species has necessitated systematic collection, documentation
and evaluation of wild Cicer species for use in chickpea
variety improvement programs. Cicer arietinum has very
narrow genetic variation, and the use of a wild relative in
chickpea breeding could provide a good opportunity for increasing the available genetic variation of cultivated chickpea.
Genetic diversity and the relationship of 71 accessions, from
the core area of chickpea origin and domestication (Southeastern Turkey), belonging to five wild annual species and one
cultivated species (Cicer arietinum) were analysed using
iPBS-retrotransposon and ISSR markers. A total of 136 scorable bands were detected using 10 ISSR primers among 71
accessions belonging to 6 species, out of which 135 were
polymorphic (99.3 %), with an average of 13.5 polymorphic
fragments per primer, whereas iPBS detected 130 bands with
100 % polymorphism with an average of 13.0 bands per
primer. C. echinospermum and C. pinnatifidum were the most
diverse among species, whereas C. arietinum exhibited lower
polymorphism. The average polymorphism information contents (PIC) value for both marker systems was 0.91. The
clustering of the accessions and species within groups was
almost similar, when iPBS and ISSR NeighborNet (NNet)
planar graphs were compared. Further detailed studies are
indispensable in order to collect Cicer germplasm, especially
C. reticulatum, from southeastern Turkey particularly, from
Karacadağ Mountain for preservation, management of this
species, and to study their genetic diversity at molecular level.
This study also demonstrates the utility and role of iPBSretrotransposons, a dominant and ubiquitous part of eukaryotic genomes, for diversity studies in wild chickpea and in
cultivated chickpea
Inter-primer binding site retrotransposon and inter-simple sequence repeat diversity among wild Lens species
Even though lentil has been an important food legume for centuries, genetic studies in lentil are still in their infancy. Genetic diversity and relationships among wild Lens species from Turkey has seldom been investigated. Additionally, a limited number of simple sequence repeat (SSR) markers have been developed for use in breeding and genetic studies of lentil crop. In this study, molecular characterization of 50 accessions mostly from Turkey, belonging to 6 wild and 1 cultivated Lens species, was performed using newly developed inter-primer binding site (iPBS) retrotransposons and inter-SSR (ISSR) markers. The 10 iPBS primers generated a total of 151 scorable bands, of which 150 were polymorphic (99.3%) with an average of 15.0 polymorphic fragments per primer. The 10 ISSR primers detected 138 scorable bands showing 100% polymorphism, with an average of 13.5 bands per primer. The average polymorphism information content (PIC) value for ISSR markers (0.97) was higher than that for iPBS markers (0.90). Lens orientalis was found to be the most diverse species, raising the possibility of wide crosses with cultivated species Lens culinaris. Cultivated varieties also showed high level of polymorphism, at 82.92% and 51.92% with ISSR and iPBS markers, respectively. Lens lamottei and Lens tomentosus were found as the least polymorphic species using both marker systems. The grouping of accessions and species within clusters were almost similar when iPBS and ISSR graphs were compared. Our data also suggested the role of iPBS-retrotransposons as ‘a universal marker’ for molecular characterization of wild and cultivated Lens species.Çukurova Üniversitesi BAP Birimi-ZF2011YL
Population structure of rice varieties used in Turkish rice breeding programs determined using simple sequence repeat and inter primer binding site retrotransposon data
Effective breeding programs based on genetic diversity are needed to broaden the genetic basis of rice (Oryza sativa L.) in Turkey. In this study, 81 commercial varieties from seven countries were studied in order to estimate the genomic relationships among them using nine interprimer binding site (iPBS)-retrotransposon and 17 simple-sequence repeat (SSR) markers. A total of 59 alleles for the SSR markers and 96 bands for the iPBS-retrotransposon markers were detected, with an average of 3.47 and 10.6 per locus, respectively. Each of the varieties could be unequivocally identified by the SSR and iPBS-retrotransposon profiles. The iPBS-retrotransposon-and SSR-based clustering were identical and closely mirrored each other, with a significantly high correlation (r = 0.73). A neighbor-joining cluster based on the combined SSR and iPBS-retrotransposon data divided the rice varieties into three clusters. The population structure was determined using the STRUCTURE software, and three populations (K = 3) were identified among the varieties studied, showing that the diversity harbored by Turkish rice varieties is low. The results indicate that iPBS-retrotransposon markers are a very powerful technique to determine the genetic diversity of rice varieties
Gramisidin A Biyosürfaktanının MCF-7 Meme Kanseri Hücre Dizisinde İnflamasyona Etkisinin Moleküler Düzeyde Araştırılması
Ekstraselüler matriksin pulmoner hipertansiyon üzerine etkilerinin hayvan modelinde araştırılması
Population structure of rice varieties commonly used in the Turkish rice breeding programs determined using simple sequence repeat and iPBS retrotransposon data
The relationship between plasma hyaluronan levels and anthracycline-related cardiotoxicity in breast cancer patients
Aim: Anthracycline-derived antineoplastic agents are used as the main form of treatment in many malignant diseases, including breast cancer and childhood cancers. Cardiotoxicity is one of the most feared life-threatening complications of cancer therapy. In the present study, we aimed to investigate the relationship between plasma hyaluronan (HA) levels and anthracycline-induced cardiotoxicity