Phylogenetic relationships, based on SSU rDNA sequences, among the didelphic genera of the family trichodoridae from Portugal

A survey of virus vector trichodorid nematodes was carried out in the central and northern regions of Portugal. Morphobio- metric identification showed the presence of trichodorid species previously reported from Portugal, except for Paratrichodorus porosus, which is reported for the first time in Continental Europe. Small subunit ribosomal DNA (SSU rDNA) sequences of ten different species occurring in Portugal were obtained and a phylogenetic analysis based on their alignment was performed to infer relationships among the different Portuguese trichodorid species and three non-indigenous populations (Nanidorus minor, P. allius and P. teres). The re- sulting phylogenetic tree is in agreement with the currently accepted classification for Trichodoridae, except for Nanidorus, which clusters together with Trichodorus species, while the genera Paratrichodorus and Trichodorus appear as two distinct groups. A better understanding of the generic groupings in the family Trichodoridae was found. Based on the new molecular analyses we herein accept Nanidorus as a valid genus

Study of the virus vector genus Trichodorus (Diphtherophorina, Trichodoridae) from the Iberian Peninsula, an apparent centre of speciation

Trichodoridae are polyphagous root ectoparasites occurring worldwide. Their major pest status is as virus vector of Tobraviruses. Currently, the family has 102 species classified within 6 genera. The genus Trichodorus is the largest in number of species (56) and predominantly occurs in temperate regions. Traditional morphology-based taxonomy revealed for Europe, a very high species diversity within the Iberian Peninsula, comprising about one fifth of all Trichodoridae described. Characteristic for this fauna is the presence of a morpho-species group within Trichodorus, characterized in males by slightly ventrally curved spicules with a mid-blade constriction with bristles and females with relatively large vaginal sclerotized pieces, quadrangular to triangular in shape. Recent surveys for Trichodoridae in cultivated and natural environments in Southern Spain and compared with the fauna from Portugal revealed four new species of Trichodorus, three of them belonging to this morpho-species group. Molecular analyses based on nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S and partial 18S gene) supported not only the new species but also the morpho-species group as a separate clade. The integrated approach of morphology based taxonomy with molecular and biogeographic data enhanced the accuracy of the observed biodiversity and strengthen the hypothesis of the Iberian Peninsula as a center of speciation.The Society of Nematologists (SON

Integrative taxonomic study of the virus vector family Trichodoridae from the Iberian Peninsula, an apparent centre of speciation

Trichodoridae are polyphagous root ectoparasites occurring worldwide. Their major pest status is as virus vector of Tobraviruses. Currently, the family has 102 species classified within 6 genera. 88% of the species belong to the didelphic genera (females with two well developed genital branches) with the genus Trichodorus being the largest in number of species (56). Trichodorus species predominantly occur in temperate regions. Surveys for trichodorids were carried out in cultivated and natural habitats in Spain and compared with the trichodorid fauna from Portugal. A comparative morphological study was carried out together with molecular analyses based on nuclear ribosomal RNA genes (D2-D3 expansion segments of 28S and partial 18S gene). Characteristic for the Iberian Peninsula is the high number of morphologically closely resembling species but clearly separated molecularly (cryptic species), characterized in males by slightly ventrally curved spicules with a mid-blade constriction with bristles and females with relatively large vaginal sclerotized pieces, quadrangular to triangular in shape. Molecular analyses demonstrated that D2-D3 expansion segments are suitable diagnostic markers for Trichodoridae. Comparative morphology and molecular analyses provide support for the Iberian Peninsula as an apparent centre of speciation. South Africa is another known centre of speciation of Trichodorus.Nematological Society of Southern Afric

Coronal Density and Temperature Profiles Calculated by Forward Modeling EUV Emission Observed by SDO/AIA

We present a model for the intensity of optically thin extreme ultraviolet (EUV) emission for a plasma atmosphere. We apply our model to the solar corona as observed using the six optically thin EUV channels of the Solar Dynamics Observatory/Atmospheric Imaging Assembly instrument. The emissivity of the plasma is calculated from the density and temperature using CHIANTI tables and the intensity is then determined by integration along the line of sight. We consider several different profiles for the radial density and temperature profiles, each of which are constrained by the observational data alone with no further physical assumptions. We demonstrate the method first by applying it to a quiet region of the corona, and then use it as the background component of a model including coronal holes, allowing the plasma densities and temperatures inside and outside the hole to be estimated. We compare our results with differential emission measure inversions. More accurate estimates for the coronal density and temperature profiles have the potential to help constrain plasma properties such as the magnetic field strength when used in combination with methods such as seismology

Prevalence and characterization of plant-parasitic nematodes in lowland and upland rice agro-ecosystems in Luzon, Philippines

The prevalence of plant-parasitic nematodes in lowland and upland rice ecosystems was compared based on soil and root samples from 30 rice fields in five provinces (Bataan, Batangas, Bulacan, Pampanga, and Pangasinan) of Luzon, Philippines. Five nematode genera (Meloidogyne, Hirschmanniella, Pratylenchus, Tylenchorhynchus, and Helicotylenchus) from lowland and nine genera (Rotylenchulus, Aphelenchoides, Criconematidae, Rotylenchus, and those found in lowland) from upland were identified. Meloidogyne graminicola was the most prevalent and abundant plant-parasitic nematode in both ecosystems. Hirschmanniella was the second most prevalent genus in lowland rice, but its density was low in upland rice. Pratylenchus was present in high densities, was not widely distributed in either upland or lowland rice fields. The three most prevalent nematode species, M. graminicola, H. oryzae, and H. mucronata, were morphologically and molecularly characterized. Molecular analyses based on D2D3, ITS1-5.8-ITS2, and 18S rDNA regions generally showed an overall congruence, low intraspecific variation and agreement with classical morphological and morphometrical identifications