Improved single-swab sample preparation for recovering bacterial and phage DNA from human skin and wound microbiomes.

BackgroundCharacterization of the skin and wound microbiome is of high biomedical interest, but is hampered by the low biomass of typical samples. While sample preparation from other microbiomes (e.g., gut) has been the subject of extensive optimization, procedures for skin and wound microbiomes have received relatively little attention. Here we describe an improved method for obtaining both phage and microbial DNA from a single skin or wound swab, characterize the yield of DNA in model samples, and demonstrate the utility of this approach with samples collected from a wound clinic.ResultsWe find a substantial improvement when processing wound samples in particular; while only one-quarter of wound samples processed by a traditional method yielded sufficient DNA for downstream analysis, all samples processed using the improved method yielded sufficient DNA. Moreover, for both skin and wound samples, community analysis and viral reads obtained through deep sequencing of clinical swab samples showed significant improvement with the use of the improved method.ConclusionUse of this method may increase the efficiency and data quality of microbiome studies from low-biomass samples

Microbial predictors of healing and short-term effect of debridement on the microbiome of chronic wounds.

Chronic wounds represent a large and growing disease burden. Infection and biofilm formation are two of the leading impediments of wound healing, suggesting an important role for the microbiome of these wounds. Debridement is a common and effective treatment for chronic wounds. We analyzed the bacterial content of the wound surface from 20 outpatients with chronic wounds before and immediately after debridement, as well as healthy skin. Given the large variation observed among different wounds, we introduce a Bayesian statistical method that models patient-to-patient variability and identify several genera that were significantly enriched in wounds vs. healthy skin. We found no difference between the microbiome of the original wound surface and that exposed by a single episode of sharp debridement, suggesting that this debridement did not directly alter the wound microbiome. However, we found that aerobes and especially facultative anaerobes were significantly associated with wounds that did not heal within 6 months. The facultative anaerobic genus Enterobacter was significantly associated with lack of healing. The results suggest that an abundance of facultative anaerobes is a negative prognostic factor in the chronic wound microbiome, possibly due to the increased robustness of such communities to different metabolic environments

To suspend or not to suspend

For many employers, the suspension of an employee literally amounts to a headache. The question which usually comes to mind is: What do I have to do before I can suspend an employee? This article investigates the nature of the suspension, the courts’ application thereof and makes recommendations on procedural aspects

Dynamical phase transition of a 1D transport process including death

Motivated by biological aspects related to fungus growth, we consider the competition of growth and corrosion. We study a modification of the totally asymmetric exclusion process, including the probabilities of injection $\alpha$ and death of the last particle $\delta$. The system presents a phase transition at $\delta_c(\alpha)$, where the average position of the last particle $$ grows as $\sqrt{t}$. For $\delta>\delta_c$, a non equilibrium stationary state exists while for $\delta<\delta_c$ the asymptotic state presents a low density and max current phases. We discuss the scaling of the density and current profiles for parallel and sequential updates.Comment: 4 pages, 5 figure

If a safety aid is present, there must be danger: the paradoxical effects of hand sanitizer during a contamination exposure task

Perceptions of danger often arise in the context of feared threat cues, but individuals also rely on other heuristics that lead them to infer danger in ambiguous situations. For example, individuals may interpret their own anxiety or safety-seeking behaviors as indicators of threat. Another potential source of danger information is the mere availability of safety aids in the environment. Although assumed to be helpful, safety aids might paradoxically elicit, rather than alleviate, anxiety. The present study was designed to assess the degree to which concern-relevant safety aids exacerbate distress. Participants (N = 71) completed several self-report measures and engaged in a contamination-related behavioral avoidance task (BAT) in the presence or absence of a 2L hand sanitizer dispenser. Results showed that participants higher in trait contamination aversion and in the presence of hand sanitizer endorsed greater inferences of danger, hypervigilance, peak BAT anxiety and disgust, BAT avoidance, and urges to wash following the BAT. Theoretical and clinical implications of the paradoxical inference of danger from the presence of safety aids are discussed

Smoking prevalence among lesbian, bisexual and queer women in Sydney remains high: analysis of trends and correlates

Introduction and Aims: To investigate smoking prevalence trends and correlates among lesbian, bisexual and queer-identifying (LBQ) women in Sydney, Australia. Design and Methods – Data from 5007 respondents to a repeated cross-sectional community survey were used to examine smoking trends between 2004 and 2014. Multinomial logistic regression was used to examine smoking correlates. Results – 30% of respondents were current smokers including 48% of 16-24 year olds. A slight decrease in all-ages smoking over time was not reflected in the youngest age group. LBQ women who smoke have fewer economic, social and psychological resources than both women who never smoke and ex-smokers. High levels of alcohol and illicit drug use are also correlated with current smoking. Discussion and conclusions – Population-wide interventions have failed to address the persistently high prevalence of smoking among this sample of LBQ women. Tailored interventions may find utility focusing on personal resilience to deal with general and sexuality-specific stressors, as well as attending to poly-substance use. Acknowledgment of LBQ women as a priority group for tobacco reduction is urgently needed. We call on tobacco control agencies to consider sexuality and gender orientation in policy and partner with LGBT community organisations to develop culturally appropriate interventions

"It's cleaner, definitely": Collaborative Process in Audio Production.

Working from vague client instructions, how do audio producers collaborate to diagnose what specifically is wrong with a piece of music, where the problem is and what to do about it? This paper presents a design ethnography that uncovers some of the ways in which two music producers co-ordinate their understanding of complex representations of pieces of music while working together in a studio. Our analysis shows that audio producers constantly make judgements based on audio and visual evidence while working with complex digital tools, which can lead to ambiguity in assessments of issues. We show how multimodal conduct guides the process of work and that complex media objects are integrated as elements of interaction by the music producers. The findings provide an understanding how people currently collaborate when producing audio, to support the design of better tools and systems for collaborative audio production in the future

User experience in an interactive music virtual reality system: An exploratory study

The Objects VR interface and study explores interactive music and virtual reality, focusing on user experience, understanding of musical functionality, and interaction issues. Our system offers spatio-temporal music interaction using 3D geometric shapes and their designed relationships. Control is provided by tracking of the hands, and the experience is rendered across a head-mounted display with binaural sound presented over headphones. The evaluation of the system uses a mixed methods approach based on semi-structured interviews, surveys and video-based interaction analysis. On average the system was positively received in terms of interview self-report, metrics for spatial presence and creative support. Interaction analysis and interview thematic analysis also revealed instances of frustration with interaction and levels of confusion with system functionality. Our results allow reflection on design criteria and discussion of implications for facilitating music engagement in virtual reality. Finally our work discusses the effectiveness of measures with respect to future evaluation of novel interactive music systems in virtual reality