Multifunction tests of a frequency domain based flutter suppression system

The process is described of analysis, design, digital implementation, and subsonic testing of an active control flutter suppression system for a full span, free-to-roll wind tunnel model of an advanced fighter concept. The design technique uses a frequency domain representation of the plant and used optimization techniques to generate a robust multi input/multi output controller. During testing in a fixed-in-roll configuration, simultaneous suppression of both symmetric and antisymmetric flutter was successfully shown. For a free-to-roll configuration, symmetric flutter was suppressed to the limit of the tunnel test envelope. During aggressive rolling maneuvers above the open-loop flutter boundary, simultaneous flutter suppression and maneuver load control were demonstrated. Finally, the flutter damping controller was reoptimized overnight during the test using combined experimental and analytical frequency domain data, resulting in improved stability robustness

Critical parameters for efficient sonication and improved chromatin immunoprecipitation of high molecular weight proteins

Solubilization of cross-linked cells followed by chromatin shearing is essential for successful chromatin immunoprecipitation (ChIP). However, this task, typically accomplished by ultrasound treatment, may often become a pitfall of the process, due to inconsistent results obtained between different experiments under seemingly identical conditions. To address this issue we systematically studied ultrasound-mediated cell lysis and chromatin shearing, identified critical parameters of the process and formulated a generic strategy for rational optimization of ultrasound treatment. We also demonstrated that whereas ultrasound treatment required to shear chromatin to within a range of 100–400 bp typically degrades large proteins, a combination of brief sonication and benzonase digestion allows for the generation of similarly sized chromatin fragments while preserving the integrity of associated proteins. This approach should drastically improve ChIP efficiency for this class of proteins

Tendon proper- and peritenon-derived progenitor cells have unique tenogenic properties.

IntroductionMultipotent progenitor populations exist within the tendon proper and peritenon of the Achilles tendon. Progenitor populations derived from the tendon proper and peritenon are enriched with distinct cell types that are distinguished by expression of markers of tendon and vascular or pericyte origins, respectively. The objective of this study was to discern the unique tenogenic properties of tendon proper- and peritenon-derived progenitors within an in vitro model. We hypothesized that progenitors from each region contribute differently to tendon formation; thus, when incorporated into a regenerative model, progenitors from each region will respond uniquely. Moreover, we hypothesized that cell populations like progenitors were capable of stimulating tenogenic differentiation, so we generated conditioned media from these cell types to analyze their stimulatory potentials.MethodsIsolated progenitors were seeded within fibrinogen/thrombin gel-based constructs with or without supplementation with recombinant growth/differentiation factor-5 (GDF5). Early and late in culture, gene expression of differentiation markers and matrix assembly genes was analyzed. Tendon construct ultrastructure was also compared after 45 days. Moreover, conditioned media from tendon proper-derived progenitors, peritenon-derived progenitors, or tenocytes was applied to each of the three cell types to determine paracrine stimulatory effects of the factors secreted from each of the respective cell types.ResultsThe cell orientation, extracellular domain and fibril organization of constructs were comparable to embryonic tendon. The tendon proper-derived progenitors produced a more tendon-like construct than the peritenon-derived progenitors. Seeded tendon proper-derived progenitors expressed greater levels of tenogenic markers and matrix assembly genes, relative to peritenon-derived progenitors. However, GDF5 supplementation improved expression of matrix assembly genes in peritenon progenitors and structurally led to increased mean fibril diameters. It also was found that peritenon-derived progenitors secrete factor(s) stimulatory to tenocytes and tendon proper progenitors.ConclusionsData demonstrate that, relative to peritenon-derived progenitors, tendon proper progenitors have greater potential for forming functional tendon-like tissue. Furthermore, factors secreted by peritenon-derived progenitors suggest a trophic role for this cell type as well. Thus, these findings highlight the synergistic potential of including these progenitor populations in restorative tendon engineering strategies

From Mentoring to Mattering: How Peer Mentoring Can Help Students Belong

Mattering—defined as the “perception that, to some degree and in any of a variety of ways, we are a significant part of the world around us” (Elliott, Kao, & Grant, 2004, p. 339)—is a social-psychological concept that has recently gained traction in higher education. Understanding mattering, college educators believe, could offer new ways to involve students in campus communities, contributing to their well-being and academic success. The present study explored the link between mattering and participating in a mentoring program for first-year students, with emphasis on the experiences of students of color and international students. The study found participating in a mentoring program could have a positive impact on students’ feelings of mattering. In addition, the study explored how students of color and international students perceived mattering relative to their white and domestic peers but found no significant differences between these students’ feelings of mattering and their peers’. Exploring mattering more extensively and in other educational settings could yield new understanding of how to increase students’ sense of belonging in college, which could contribute to student persistence and other positive developments

Flutter suppression using eigenspace freedoms to meet requirements

A constrained optimization methodology has been developed which allows specific use of eigensystem freedoms to meet design requirements. A subset of the available eigenvector freedoms was employed. The eigenvector freedoms associated with a particular closed-loop eigenvalue are coefficients of basis vectors which span the subspace in which that closed-loop vector must lie. Design requirements are included as a vector of inequality constraints. The procedure was successfully applied to develop an unscheduled controller which stabilizes symmetric flutter of an aeroelastic vehicle to a dynamic pressure 44 percent above the open-loop flutter point. The design process proceeded from full-state feedback to the inclusion of a full-order observer to the selection of an eighth-order controller which preserved the full-state sensitivity characteristics. Only a subset of the design freedoms was utilized (i.e., assuming full-state feedback only four out of 26 eigenvectors were used, and no variations were made in the closed-loop eigenvalues). Utilization of additional eigensystem freedoms could further improve the controller

Engineering Polymer Informatics

The poster describes a strategy of for the development of polymer informatics. In particular, the development of polymer markup language, a polymer ontology and natural language processing tools for polymer literature

Economic impact of the Florida cultured hard clam industry

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Design, test, and evaluation of three active flutter suppression controllers

Three control law design techniques for flutter suppression are presented. Each technique uses multiple control surfaces and/or sensors. The first method uses traditional tools (such as pole/zero loci and Nyquist diagrams) for producing a controller that has minimal complexity and which is sufficiently robust to handle plant uncertainty. The second procedure uses linear combinations of several accelerometer signals and dynamic compensation to synthesize the model rate of the critical mode for feedback to the distributed control surfaces. The third technique starts with a minimum-energy linear quadratic Gaussian controller, iteratively modifies intensity matrices corresponding to input and output noise, and applies controller order reduction to achieve a low-order, robust controller. The resulting designs were implemented digitally and tested subsonically on the active flexible wing wind-tunnel model in the Langley Transonic Dynamics Tunnel. Only the traditional pole/zero loci design was sufficiently robust to errors in the nominal plant to successfully suppress flutter during the test. The traditional pole/zero loci design provided simultaneous suppression of symmetric and antisymmetric flutter with a 24-percent increase in attainable dynamic pressure. Posttest analyses are shown which illustrate the problems encountered with the other laws