811 research outputs found
Comparing Catalysts of the Direct Synthesis of Hydrogen Peroxide in Organic Solvent: is the Measure of the Product an Issue?
The direct synthesis of hydrogen peroxide has been for about 20 years a hot topic in \u201cgreen\u201d catalysis. Several methods, which are well established to measure the concentration of hydrogen peroxide in water are also applied to the analysis of reaction mixtures from the direct synthesis of H2O2. However, this step could not be always straightforward, because these mixtures contain almost invariably organic solvents and, sometimes, selectivity enhancers which can interfere in some, at the least, of the most popular titrimetric methods. This work presents a comparative investigation of iodometry, cerimetry, permanganometry (titrimetric methods) and spectrophotometric analysis of TiIV/H2O2 adduct, as applied to analysis of hydrogen peroxide produced by its direct synthesis. They account for more than 90 % of the competent literature since 2000. Their pros and cons are highlighted to provide a guideline for the choice of the best possible method of analysis and for the comparison of catalytic results assessed in different ways in the context of the direct synthesis of hydrogen peroxide
Cadmium, Lead and Chromium in Large Game: A Local-Scale Exposure Assessment for Hunters Consuming Meat and Liver of Wild Boar.
Towards a time-reversal mirror for quantum systems
The reversion of the time evolution of a quantum state can be achieved by
changing the sign of the Hamiltonian as in the polarization echo experiment in
NMR. In this work we describe an alternative mechanism inspired by the acoustic
time reversal mirror. By solving the inverse time problem in a discrete space
we develop a new procedure, the perfect inverse filter. It achieves the exact
time reversion in a given region by reinjecting a prescribed wave function at
its periphery.Comment: 6 pages, 4 figures. Introduction modified, references added, one
figure added to improve the discussio
Histone Deacetylase and Microtubules as Targets for the Synthesis of Releasable Conjugate Compounds
Design and synthesis of an HDAC inhibitor and its merger with three tubulin binders to create releasable conjugate compounds is described. The biological evaluation includes: a) in vitro reactivity with glutathione, b) antiproliferative activity, c) cell cycle analysis and d) quantification of protein acetylation. The cellular pharmacology study indicated that the HDAC-inhibitor-drug conjugates retained antimitotic and proapoptotic activity with a reduced potenc
Role of combined DWIBS/3D-CE-T1w whole-body MRI in tumor staging: Comparison with PET-CT
Objectives: To assess the diagnostic performance of whole-body magnetic resonance imaging (WB-MRI)
by diffusion-weighted whole-body imaging with background body signal suppression (DWIBS) in malignant
tumor detection and the potential diagnostic advantages in generating fused DWIBS/3D-contrast
enhanced T1w (3D-CE-T1w) images.
Methods: 45 cancer patients underwent 18F-FDG PET-CT and WB-MRI for staging purpose. Fused
DWIBS/3D-CE T1w images were generated off-line. 3D-CE-T1w, DWIBS images alone and fused with
3D-CE T1w were compared by two readers groups for detection of primary diseases and local/distant
metastases. Diagnostic performance between the three WB-MRI data sets was assessed using receiver
operating characteristic (ROC) curve analysis. Imaging exams and histopathological results were used as
standard of references.
Results: Areas under the ROC curves of DWIBS vs. 3D-CE-T1w vs. both sequences in fused fashion were
0.97, 0.978, and 1.00, respectively. The diagnostic performance in tumor detection of fused DWIBS/3DCE-
T1w images were statistically superior to DWIBS (p < 0.001) and 3D-CE-T1w (p
≤
0.002); while the
difference between DWIBS and 3D-CE-T1w did not show statistical significance difference. Detection
rates of malignancy did not differ between WB-MRI with DWIBS and 18F-FDG PET-CT.
Conclusion: WB-MRI with DWIBS is to be considered as alternative tool to conventional whole-body
methods for tumor staging and during follow-up in cancer patients
ROSES, the only RObotic System for any Endovascular Surgery, Including the Control of an Animated Catheter Characterized by the Presence of two Controlled Curvatures
The paper presents ROSES, its robotic components, the different devices, not necessarily sterile, and its disposables, suitable for any endovascular procedure, both actually performed and presently not assisted by any robotic system, and open in the future for new application yet to come, such as what will be allowed by the new animated catheter. In fact, this is due to the mechanical configuration of the robotic actuators based on a peculiar gear train which presents a big passage hole which allows both the passage of big catheters and even hemostasis valves, as well as full control of very small catheters and guide wires. The system measures forces opposed by the body showing their value both numerically and analogically without the need of any. special tool, measures length of penetration of each catheter and guide wires recording their value. Thus, it may become in future, connected to a work station that will register in real time also the fluoroscopic images, a kind of black box of endovascular surgeries, separating completely doctor and nurses from the patient, using also cameras and microphones to replace the physical contact with the patient
Pharmacological characterization of a new Ca2+ sensitizer
The benzimidazole molecule was modified to synthesize a Ca(2+) sensitizer devoid of additional effects associated with Ca(2+) overload. Newly synthesized compounds, termed 1, 2, 3, 4, and 5, were evaluated in spontaneously beating and electrically driven atria from reserpine-treated guinea pigs. Compound 3 resulted as the most effective positive inotropic agent, and experiments were performed to study its mechanism of action. In spontaneously beating atria, the inotropic effect of 3 was concentration-dependent (3.0 microM-0.3 mM). Compound 3 was more potent and more active than the structurally related Ca(2+) sensitizers sulmazole and caffeine, but unlike them it did not increase the heart rate. In electrically driven atria, the inotropic activity of 3 was well preserved and it was not inhibited by propranolol, prazosin, ranitidine, pyrilamine, carbachol, adenosine deaminase, or ruthenium red. At high concentrations (0.1-1.0 mM) 3 inhibited phosphodiesterase-III, whereas it did not affect Na(+)/K(+)-ATPase, sarcolemmal Ca(2+)-ATPase, Na(+)/Ca(2+) exchange carrier, or sarcoplasmic reticulum Ca(2+) pump activities of guinea pig heart. In skinned fibers obtained from guinea pig papillary muscle and skeletal soleus muscle, compound 3 (0.1 mM, 1 mM) shifted the pCa/tension relation curve to the left, with no effect on maximal tension and no signs of toxicity. Compound 3 did not influence the basal or raised tone of guinea pig isolated aorta rings, whose cells do not contain the contractile protein troponin. The present results indicate that the inotropic effect of compound 3 seems to be primarily sustained by sensitization of the contractile proteins to Ca(2+)
Despite lost contractility, a sub-population of rat muscle fibers maintains an assessable excitation-contraction coupling mechanism after long-standing denervation
J Neuropathol Exp Neurol. 2009 Dec;68(12):1256-68.
A subpopulation of rat muscle fibers maintains an assessable excitation-contraction coupling mechanism after long-standing denervation despite lost contractility.
Squecco R, Carraro U, Kern H, Pond A, Adami N, Biral D, Vindigni V, Boncompagni S, Pietrangelo T, Bosco G, Fanò G, Marini M, Abruzzo PM, Germinario E, Danieli-Betto D, Protasi F, Francini F, Zampieri S.
Source
Interuniversitary Institute of Myology, Chieti, Italy.
Abstract
To define the time course and potential effects of electrical stimulation on permanently denervated muscle, we evaluated excitation-contraction coupling (ECC) of rat leg muscles during progression to long-term denervation by ultrastructural analysis, specific binding to dihydropyridine receptors, ryanodine receptor 1 (RYR-1), Ca channels and extrusion Ca pumps, gene transcription and translation of Ca-handling proteins, and in vitro mechanical properties and electrophysiological analyses of sarcolemmal passive properties and L-type Ca current (ICa) parameters. We found that in response to long-term denervation: 1) isolated muscle that is unable to twitch in vitro by electrical stimulation has very small myofibers but may show a slow caffeine contracture; 2) only roughly half of the muscle fibers with "voltage-dependent Ca channel activity" are able to contract; 3) the ECC mechanisms are still present and, in part, functional; 4)ECC-related gene expression is upregulated; and 5) at any time point, there are muscle fibers that are more resistant than others to denervation atrophy and disorganization of the ECC apparatus. These results support the hypothesis that prolonged "resting" [Ca] may drive progression of muscle atrophy to degeneration and that electrical stimulation-induced [Ca] modulation may mimic the lost nerve influence, playing a key role in modifying the gene expression of denervated muscle. Hence, these data provide a potential molecular explanation for the muscle recovery that occurs in response to rehabilitation strategies developed based on empirical clinical observations.
PMID:
19915489
[PubMed - indexed for MEDLINE
Ocorrência de doenças fúngicas em cultivares de trigo com ciclos diferentes em Passo Fundo, RS, em 2014.
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