91 research outputs found
NEW PLATE MEDIUM FOR SCREENING AND PRESUMPTIVE IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS
A new selective, differential plating medium to screen the common gram-negative urinary tract pathogens is
described. The medium combines adonitol fermentation, phenylalanine deaminase, and P-glucuronidase tests
and allows the indole and cytochrome oxidase tests to be performed directly from the plates. High-level
agreement with individual conventional tests was recorded in comparative studies with 504 cultures of
gram-negative rods. There was 100% agreement, except for the Providencia spp. indole spot test (61.6%
agreement). Adonitol fermentation by Providencia species could not be determined. Escherichia coli, Klebsiella
pneumoniae, Proteus mirabilis, and Pseudomonas aeruginosa were identified with a high efficiency (100, 85.7,
83.5, and 100% agreement, respectively) without further testing. There was 96% overall agreement for the 267
infected urine samples tested
T-MOD PATHWAY, A REDUCED SEQUENCE FOR IDENTIFICATION OF GRAM-NEGATIVE URINARY-TRACT PATHOGENS
In this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and
differential isolation medium which allows accurate presumptive identification of the most common gramnegative
bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762
independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and
trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify
the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis.
The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed
identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and
1.2% after an identification gallery
Sistemas inteligentes de embalagens utilizando filmes de quitosana como indicador colorimétrico de temperatura
Inproved susceptibilities to cinoxacin and other antimicrobial agents of some Providencia species re-identified on the basis of polyhdric alcohols fermentation
Determinatins of tissue levels of cefatrizine in blood, longs and bronchi.
A study was carried out in 12 patients, divided into two groups of 6, to determine tissue levels of cefatrizine in lung (group I) and bronchial secretions (group II) following a single oral dose of 500 mg. In group I, specimens of blood and lung tissue were collected after 2 h from one subgroup of 3 patients and after 3 h from the other subgroup of 3. Average levels were 8.5 and 7.0 mcg/ml for blood and 1.2 and 1.4 mcg/ml for lung tissue respectively. In group II blood and bronchial secretion concentrations were evaluated at the 2nd, 3rd and 6th hours from administration. Average values were 9.1 and 7.7 mcg/ml in blood at 2h and 3h respectively, whereas the average bronchial secretion concentration at the 3rd hour was 10.4 mcg/ml in the first subgroup. In the second subgroup the mean level in blood collected at the 2nd hour was 8.9 mcg/ml, and 2.5 and 4.1 mcg/ml respectively in blood and bronchial secretions at the 6th hour. Cefatrizine levels in bronchial secretions were higher than those in blood at both the 3rd and the 6th hour from administration: this kinetic peculiarity of the drug will doubtless play an important role in the therapeutic efficacy of cefatrizine
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