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Abstract
In this paper, we describe a reduced sequence of identification that includes T-mod medium, a selective and
differential isolation medium which allows accurate presumptive identification of the most common gramnegative
bacteria encountered in urine samples. The present study, performed on bacteria isolated from 1,762
independent urine samples, has shown that a few selected tests (lysine and ornithine decarboxylase, urease and
trehalose fermentation tests) improve the identification accuracy of T-mod, making it possible both to identify
the less frequent species and to prevent some misidentifications of Klebsiella pneumoniae and Proteus mirabilis.
The proposed work flow agreed with conventional identification protocols to a 99.3% extent and allowed
identification of 87.4% of the isolates directly from the primary plate, 11.4% after 1 to 3 additional tests, and
1.2% after an identification gallery