55 research outputs found
Expression of functional toll like receptor 4 in estrogen receptor/progesterone receptor-negative breast cancer
Identification of a Novel Class of Farnesylation Targets by Structure-Based Modeling of Binding Specificity
Farnesylation is an important post-translational modification catalyzed by farnesyltransferase (FTase). Until recently it was believed that a C-terminal CaaX motif is required for farnesylation, but recent experiments have revealed larger substrate diversity. In this study, we propose a general structural modeling scheme to account for peptide binding specificity and recapitulate the experimentally derived selectivity profile of FTase in vitro. In addition to highly accurate recovery of known FTase targets, we also identify a range of novel potential targets in the human genome, including a new substrate class with an acidic C-terminal residue (CxxD/E). In vitro experiments verified farnesylation of 26/29 tested peptides, including both novel human targets, as well as peptides predicted to tightly bind FTase. This study extends the putative range of biological farnesylation substrates. Moreover, it suggests that the ability of a peptide to bind FTase is a main determinant for the farnesylation reaction. Finally, simple adaptation of our approach can contribute to more accurate and complete elucidation of peptide-mediated interactions and modifications in the cell
Effects of the timing of tourniquet release in cemented total knee arthroplasty: a systematic review and meta-analysis of randomized controlled trials
Application of Gibbs’ model to urban drainage networks: a case study in southwestern Chicago, USA
Tracheoinnominate Artery Fistula Treated With Endovascular Stent Graft at a Level I Trauma Center
An analysis of factors associated with graft topographic outcomes after deep anterior lamellar keratoplasty
20Â kHz toluene planar laser-induced fluorescence imaging of a jet in nearly sonic crossflow
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