Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part II [Morfologiczna charakterystyka Cyclamen sp. rosnacych w Turcji w warunkach naturalnych: Czesc II]

The morphology of 279 accessions of Cyclamen sp. growing naturally in Tur-key, namely C. alpinum (syn. C. trochopteranthum), C. graecum, C. hederifolium (syn. C. neapolitanum) and C. mirabile, was characterized. Plants with intact tubers were collected from locations in Antalya, Isparta, Aydın, Muğla, İzmir and Denizli, determined by GPS, where they grow naturally in spring and autumn. The morphology of the four Cyclamen species was characterized using one year old regenerated plants based on 27 morphological traits (13 flower, 11 leaf, 2 plant, 1 tuber). There were distinct differ-ences among these accessions related to petal colour, pedicel length, leaf length and width, leaf shape, and tuber diameter. Even though principle component analysis con-firmed the grouping of characters into species-specific clusters, a wider range of morpho-logical data as well as molecular data are needed for more reliable conclusions to be drawn about the classification of these Cyclamen species. © by Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part I

Twenty cyclamen taxa belonging to the Primulaceae originate from the Mediterranean region and grow under trees and bushes. There are 10 cyclamen species growing naturally in Turkey, five of which are endemic. In this study, intact cyclamen plants were collected from nature with their tubers intact. Collection took place in spring and autumn in Adana, Osmaniye, Kahramanmaraş, I'zmir and Eskişehir provinces, 50 samples per location. Sampled plants were propagated and cultivated in Adana. The morphology of four Cyclamen species (C. persicum Mill., C. cilicium Boiss.e.Heldr., C. pseudibericum Hildebr. and C. coum Mill.) was characterized using 1-year-old regenerated plants. A total of 27 phenotypic characters (13 flower, 11 leaf, 2 plant, 1 tuber) were evaluated based on a detailed descriptor's list. In addition to these morphological observations, 13 quantitative traits (7 flower, 5 leaf, 1 tuber) were measured. The measurements related to morphological characteristics had a wide range of variation, including in tuber diameter, leaf length and width, petal length, petal color, and leaf shape, indicating the vast morphological differences among these four cyclamen species. In most cases, the use of principal component analysis confirmed the grouping of characters into species-specific clusters although one or two clusters could not differentiate species, indicating that morphological and cluster analyses alone are not enough for characterizing this complex Cyclamen germplasm and that molecular techniques may reveal more intricate and useful relationships. © 2015 South African Association of Botanists

Erratum to: Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers (Biochemical Genetics, (2017), 55, 1, (87-102), 10.1007/s10528-016-9770-9)

PubMedID: 27639865The affiliation of Jaime A. Teixeira da Silva was incorrect in the original publication. The correct information is published with this erratum. © 2016, Springer Science+Business Media New York

Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers (vol 55, pg 87, 2017)

WOS: 000391977700009PubMed ID: 27639865

Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers

PubMed ID: 27604272The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydın, Muğla, İzmir, Denizli, Kahramanmaraş, Osmaniye, Eskişehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together. © 2016 Springer Science+Business Media New Yor

Development of an efficient regeneration protocol for four Cyclamen species endemic to Turkey

In this study, embryo-like structures (ELSs) were induced in four endemic Turkish Cyclamen species (C. cilicium Boiss. et Heldr., C. parviflorum Pobed., C. mirabile Hildebr. and C. pseudibericum Hildebr.) in the presence of 13 combinations of two plant growth regulators (PGRs) (2,4-dichlorophenoxyacetic acid and 6-(gamma,gamma-dimethylallylamino)purine) and four explant types (ovules, ovaries, leaves and petioles). The ratio of callus induction, different stages of ELS formation and the conversion of ELSs to plantlets were quantified. The most effective explant types for callus induction were leaves (56 % for C. cilicium and 59 % for C. parviflorum) and petioles (80 % for C. mirabile and 100 % for C. pseudibericum). Callus growth from the leaves and petioles of C. cilicium was 30 days earlier than that of C. mirabile and C. pseudibericum. In contrast, most callus formed from the petioles of C. pseudibericum (100 %) in medium with 2.5 mg l(-1) 2,4-D and 1 mg l(-1) 2iP. The highest number of ELSs was obtained succesfully from petioles (2.5 mg l(-1) 2,4-D and 1 mg l(-1) 2iP) and ovaries (2.5 mg l(-1) 2,4-D and 0.5 mg l(-1) 2iP) of C. pseudibericum, in 39 % and as 32 % of explants, respectively. The percentage conversion of ELSs to plantlets was 38, 31, 16 and 15 % for C. mirabile, C. cilicium, C. pseudibericum and C. parviflorum, respectively. The plantlets were successfully acclimatized in the greenhouse with 54, 70, 63 and 25 % of C. cilicium, C. mirabile, C. pseudibericum and C. parviflorum plantlets, respectively surviving after transfer to ex vitro conditions. This paper describes a unique, reliable and consistent protocol for the induction of ELSs from four endangered endemic Turkish Cyclamen species, opening up the possibility of preserving these valuable genetic resources in vitro and also other applied biotechnologies that rely on a stable embryogenic or callus-based protocol

Molecular Characterization of Cyclamen Species Collected from Different Parts of Turkey by RAPD and SRAP Markers

The genus Cyclamen (family Myrsinaceae) contains about 20 species, most of which occur in the Mediterranean region. Turkey has critically important Cyclamen genetic resources. Molecular characterization of plant materials collected from different regions of Turkey in which Cyclamen species grow naturally, namely Adana, Antalya, Aydin, Mugla, Izmir, Denizli, Kahramanmaras, Osmaniye, Eskisehir, Trabzon, and Rize provinces, was performed using RAPD and SRAP markers. DNA was successfully amplified by 30 RAPD primers and 14 SRAP primer pairs. Among the 470 bands generated by the RAPD primers, 467 were polymorphic. The number of bands detected by a single primer set ranged from 11 to 22 (average of 15.6). The percentage polymorphism was 99.3 % based on the RAPD data. In the SRAP analysis, a total of 216 bands were generated, showing 100 % polymorphism. The number of bands detected by a single primer set ranged from 9 to 22 (average of 15.4). All data were scored and UPGMA dendrograms were constructed with similar results in both marker systems, i.e., different species from nine provinces of Turkey were separated from each other in the dendrograms with the same species being clustered together

MORPHOLOGICAL CHARACTERIZATION OF Cyclamen sp GROWN NATURALLY IN TURKEY: PART II

WOS: 000391345700015The morphology of 279 accessions of Cyclamen sp. growing naturally in Turkey, namely C. alpinum (syn. C. trochopteranthum), C. graecum, C. hederifolium (syn. C. neapolitanum) and C. mirabile, was characterized. Plants with intact tubers were collected from locations in Antalya, Isparta, Aydin, Mugla, Izmir and Denizli, determined by GPS, where they grow naturally in spring and autumn. The morphology of the four Cyclamen species was characterized using one year old regenerated plants based on 27 morphological traits (13 flower, 11 leaf, 2 plant, 1 tuber). There were distinct differences among these accessions related to petal colour, pedicel length, leaf length and width, leaf shape, and tuber diameter. Even though principle component analysis confirmed the grouping of characters into species-specific clusters, a wider range of morphological data as well as molecular data are needed for more reliable conclusions to be drawn about the classification of these Cyclamen species.Council of Scientific and Technological Research of Turkey [TUBITAK 110O102]The authors are grateful to the Council of Scientific and Technological Research of Turkey for financial support (Project No.: TUBITAK 110O102) and to Prof Dr. Sedat Serce to his kindly help with statistical analysis

Somatic embryogenesis of Turkish Cyclamen persicum Mill

Cyclamen sp. occupies a wide swathe of habitats across Turkey. Ten wild Cyclamen species grow naturally in Turkey, some being endemic. Due to this genetic variation, wild Cyclamen sp. with traits such as flower shape and colour, leaf shape and colour and disease resistance make these species important for cyclamen breeders. In this study, the potential of somatic embryogenesis from different explants (ovules, divided ovary parts, leaves and petiole segments) of 15 separate genotypes from one wild species (Cyclamen persicum Mill.) was studied. Explants were cultured on medium containing half-strength Murashige and Skoog macro- and micro-elements and 2.0mgl-1 2,4-dichlorophenoxyacetic acid and 0.8mgl-1 6-(?,?-dimethylallylamino) purine for inducing embryogenic callus. Embryogenic potential differed significantly between explants and genotypes. Although callus was most prolific from petiole explants, somatic embryos formed most efficiently on ovary explants. The ability of petiole, ovary, ovule and leaf explants, when averaged for the 15 genotypes, to form callus, was 34.3, 30.16, 26.6 and 15.6%, respectively while the percentages of somatic embryos formed were 11.3, 8.00, 4.16 and 2.83% of ovary, petiole, leaf and ovule explants, respectively. © 2014 Elsevier B.V.TOVAG 110O102This research was supported by TUBITAK (The Scientific and Technical Research Council of Turkey) (Project no.: TOVAG 110O102 ) project. -

Morphological characterization of Cyclamen sp. grown naturally in Turkey: Part II [Morfologiczna charakterystyka Cyclamen sp. rosnacych w Turcji w warunkach naturalnych: Czesc II]

The morphology of 279 accessions of Cyclamen sp. growing naturally in Tur-key, namely C. alpinum (syn. C. trochopteranthum), C. graecum, C. hederifolium (syn. C. neapolitanum) and C. mirabile, was characterized. Plants with intact tubers were collected from locations in Antalya, Isparta, Aydın, Muğla, İzmir and Denizli, determined by GPS, where they grow naturally in spring and autumn. The morphology of the four Cyclamen species was characterized using one year old regenerated plants based on 27 morphological traits (13 flower, 11 leaf, 2 plant, 1 tuber). There were distinct differ-ences among these accessions related to petal colour, pedicel length, leaf length and width, leaf shape, and tuber diameter. Even though principle component analysis con-firmed the grouping of characters into species-specific clusters, a wider range of morpho-logical data as well as molecular data are needed for more reliable conclusions to be drawn about the classification of these Cyclamen species. © by Wydawnictwo Uniwersytetu Przyrodniczego w Lublinie, Lublin 2016