Investigation of various factors on the germination of chia seeds sprouts (Salvia hispanica L.)

ArticleSalvia hispanica L. is capable to produce a large amount of green matter, which can be used as a source of biologically active substances. The purpose of this research was to select the optimal factors for the chia seed sprouts (Salvia hispanica L.) germination. Dark variety chia seeds (100 grains/sample) were investigated. The most significant factors for the process of sprouting were selected as the study factors, such as the water mass fraction, the temperature and the light exposure for seed germination. The output parameters of the experiment were seed germination energy, germination of seeds, speed of germination and seedling vigor. It was revealed that the mass fraction of added water had the greatest influence on the growing process of chia seed sprouts. The optimal amount of water for producing the chia seed sprouts was in the average of 4 mL/sample. As a result, it was noted that an insufficient or excessive amount of water had a negative effect on the chia seed sprouts germination. The optimum temperature for germination of chia seed sprouts was 25 °C. The optimal light factor was also determined; in particular light exposure peaks occur in the red spectrum with a wavelength of 660 nm and a blue spectrum with a wavelength of 450 nm

Молекулярный анализ гена GID1 у Dasypyrum villosum и создание ДНК-маркера для его идентификации

Dasypyrum villosum is an annual cereal used as a donor of agronomic traits for wheat. Productivity is one of the most important traits that breeding is aimed at. It is a very complex trait, the formation of which is influenced by many different factors, both internal (the genotype of the plant) and external. The genes responsible for the gibberellin sensitivity played a large role in multiplying yields of cereal crops. Another such gene is the Gid1, which encodes a receptor for gibberellins. This article compares the DNA sequences of the Gid1 gene obtained from six Dasypyrum villosum samples. Using a sequence of wheat and rye taken from the GenBank database (NCBI), we selected primers for regions of different genomes (A, B, and D subgenomes of wheat and the R genome of rye), and carried out a polymerase chain reaction on D. villosum accessions of diverse geographical origin. The resulting PCR product was sequenced by an NGS method. Based on the assembled sequences, DNA markers have been created that make it possible to differentiate these genes of the V genome and homologous genes of wheat origin. Using monosomic addition, substitution, and translocation wheat lines, the localization of the Gid1 gene of D. villosum was established on the long arm of the first V chromosome. A phenotypic assessment of common wheat lines carrying substituted, translocated, or added D. villosum chromosomes in their karyotype was performed. Tendency of disappearance of the first chromosome of D. villosum in the lines with added chromosomes was revealed.Dasypyrum villosum (VV) - однолетний злак, зарекомендовавший себя в качестве донора хозяйственно-ценных признаков для пшеницы. Один из важнейших показателей, на который направлена селекция,- урожайность, являющаяся сложным, комплексным признаком. На его формирование влияет множество различных факторов. Большую роль в росте урожайности злаковых культур сыграли гены, регулирующие физиологический ответ растений на гиббереллины, одним из которых стал ген Gid1 , являющийся рецептором активных форм этих фитогормонов. Приведено сравнение частичных ДНК-последовательностей гена Gid1 , секвенированных у двух образцов Dasypyrum villosum . Используя последовательности пшеницы и ржи, взятые из базы данных GenBank (NCBI), подобрали праймеры на участки разных геномов (субгеномы А, В и D пшеницы и геном R ржи) и провели полимеразную цепную реакцию на образцах дазипирума мохнатого различного происхождения. Полученный ПЦР-продукт был секвенирован методом NGS. На основе секвенированных нуклеотидных последовательностей создан ДНК-маркер, позволяющий дифференцировать данные гены генома V и гомологичные гены пшеничного происхождения. С использованием моносомно-дополненных, замещенных и транслоцированных линий пшеницы впервые установлена локализация гена Gid1 на хромосомах Dasypyrum villosum . Показано расположение данного гена на длинном плече первой хромосомы генома V (1VL). Проведена фенотипическая оценка линий мягкой пшеницы, имеющих в своем кариотипе замещенные, транслоцированные или дополненные хромосомы Dasypyrum villosum

Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980�2015: a systematic analysis for the Global Burden of Disease Study 2015

Background Improving survival and extending the longevity of life for all populations requires timely, robust evidence on local mortality levels and trends. The Global Burden of Disease 2015 Study (GBD 2015) provides a comprehensive assessment of all-cause and cause-specific mortality for 249 causes in 195 countries and territories from 1980 to 2015. These results informed an in-depth investigation of observed and expected mortality patterns based on sociodemographic measures. Methods We estimated all-cause mortality by age, sex, geography, and year using an improved analytical approach originally developed for GBD 2013 and GBD 2010. Improvements included refinements to the estimation of child and adult mortality and corresponding uncertainty, parameter selection for under-5 mortality synthesis by spatiotemporal Gaussian process regression, and sibling history data processing. We also expanded the database of vital registration, survey, and census data to 14�294 geography�year datapoints. For GBD 2015, eight causes, including Ebola virus disease, were added to the previous GBD cause list for mortality. We used six modelling approaches to assess cause-specific mortality, with the Cause of Death Ensemble Model (CODEm) generating estimates for most causes. We used a series of novel analyses to systematically quantify the drivers of trends in mortality across geographies. First, we assessed observed and expected levels and trends of cause-specific mortality as they relate to the Socio-demographic Index (SDI), a summary indicator derived from measures of income per capita, educational attainment, and fertility. Second, we examined factors affecting total mortality patterns through a series of counterfactual scenarios, testing the magnitude by which population growth, population age structures, and epidemiological changes contributed to shifts in mortality. Finally, we attributed changes in life expectancy to changes in cause of death. We documented each step of the GBD 2015 estimation processes, as well as data sources, in accordance with Guidelines for Accurate and Transparent Health Estimates Reporting (GATHER). Findings Globally, life expectancy from birth increased from 61·7 years (95 uncertainty interval 61·4�61·9) in 1980 to 71·8 years (71·5�72·2) in 2015. Several countries in sub-Saharan Africa had very large gains in life expectancy from 2005 to 2015, rebounding from an era of exceedingly high loss of life due to HIV/AIDS. At the same time, many geographies saw life expectancy stagnate or decline, particularly for men and in countries with rising mortality from war or interpersonal violence. From 2005 to 2015, male life expectancy in Syria dropped by 11·3 years (3·7�17·4), to 62·6 years (56·5�70·2). Total deaths increased by 4·1 (2·6�5·6) from 2005 to 2015, rising to 55·8 million (54·9 million to 56·6 million) in 2015, but age-standardised death rates fell by 17·0 (15·8�18·1) during this time, underscoring changes in population growth and shifts in global age structures. The result was similar for non-communicable diseases (NCDs), with total deaths from these causes increasing by 14·1 (12·6�16·0) to 39·8 million (39·2 million to 40·5 million) in 2015, whereas age-standardised rates decreased by 13·1 (11·9�14·3). Globally, this mortality pattern emerged for several NCDs, including several types of cancer, ischaemic heart disease, cirrhosis, and Alzheimer's disease and other dementias. By contrast, both total deaths and age-standardised death rates due to communicable, maternal, neonatal, and nutritional conditions significantly declined from 2005 to 2015, gains largely attributable to decreases in mortality rates due to HIV/AIDS (42·1, 39·1�44·6), malaria (43·1, 34·7�51·8), neonatal preterm birth complications (29·8, 24·8�34·9), and maternal disorders (29·1, 19·3�37·1). Progress was slower for several causes, such as lower respiratory infections and nutritional deficiencies, whereas deaths increased for others, including dengue and drug use disorders. Age-standardised death rates due to injuries significantly declined from 2005 to 2015, yet interpersonal violence and war claimed increasingly more lives in some regions, particularly in the Middle East. In 2015, rotaviral enteritis (rotavirus) was the leading cause of under-5 deaths due to diarrhoea (146�000 deaths, 118�000�183�000) and pneumococcal pneumonia was the leading cause of under-5 deaths due to lower respiratory infections (393�000 deaths, 228�000�532�000), although pathogen-specific mortality varied by region. Globally, the effects of population growth, ageing, and changes in age-standardised death rates substantially differed by cause. Our analyses on the expected associations between cause-specific mortality and SDI show the regular shifts in cause of death composition and population age structure with rising SDI. Country patterns of premature mortality (measured as years of life lost YLLs) and how they differ from the level expected on the basis of SDI alone revealed distinct but highly heterogeneous patterns by region and country or territory. Ischaemic heart disease, stroke, and diabetes were among the leading causes of YLLs in most regions, but in many cases, intraregional results sharply diverged for ratios of observed and expected YLLs based on SDI. Communicable, maternal, neonatal, and nutritional diseases caused the most YLLs throughout sub-Saharan Africa, with observed YLLs far exceeding expected YLLs for countries in which malaria or HIV/AIDS remained the leading causes of early death. Interpretation At the global scale, age-specific mortality has steadily improved over the past 35 years; this pattern of general progress continued in the past decade. Progress has been faster in most countries than expected on the basis of development measured by the SDI. Against this background of progress, some countries have seen falls in life expectancy, and age-standardised death rates for some causes are increasing. Despite progress in reducing age-standardised death rates, population growth and ageing mean that the number of deaths from most non-communicable causes are increasing in most countries, putting increased demands on health systems. Funding Bill & Melinda Gates Foundation. © 2016 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY licens

Global, regional, and national life expectancy, all-cause mortality, and cause-specific mortality for 249 causes of death, 1980�2015: a systematic analysis for the Global Burden of Disease Study 2015

Background Improving survival and extending the longevity of life for all populations requires timely, robust evidence on local mortality levels and trends. The Global Burden of Disease 2015 Study (GBD 2015) provides a comprehensive assessment of all-cause and cause-specific mortality for 249 causes in 195 countries and territories from 1980 to 2015. These results informed an in-depth investigation of observed and expected mortality patterns based on sociodemographic measures. Methods We estimated all-cause mortality by age, sex, geography, and year using an improved analytical approach originally developed for GBD 2013 and GBD 2010. Improvements included refinements to the estimation of child and adult mortality and corresponding uncertainty, parameter selection for under-5 mortality synthesis by spatiotemporal Gaussian process regression, and sibling history data processing. We also expanded the database of vital registration, survey, and census data to 14�294 geography�year datapoints. For GBD 2015, eight causes, including Ebola virus disease, were added to the previous GBD cause list for mortality. We used six modelling approaches to assess cause-specific mortality, with the Cause of Death Ensemble Model (CODEm) generating estimates for most causes. We used a series of novel analyses to systematically quantify the drivers of trends in mortality across geographies. First, we assessed observed and expected levels and trends of cause-specific mortality as they relate to the Socio-demographic Index (SDI), a summary indicator derived from measures of income per capita, educational attainment, and fertility. Second, we examined factors affecting total mortality patterns through a series of counterfactual scenarios, testing the magnitude by which population growth, population age structures, and epidemiological changes contributed to shifts in mortality. Finally, we attributed changes in life expectancy to changes in cause of death. We documented each step of the GBD 2015 estimation processes, as well as data sources, in accordance with Guidelines for Accurate and Transparent Health Estimates Reporting (GATHER). Findings Globally, life expectancy from birth increased from 61·7 years (95 uncertainty interval 61·4�61·9) in 1980 to 71·8 years (71·5�72·2) in 2015. Several countries in sub-Saharan Africa had very large gains in life expectancy from 2005 to 2015, rebounding from an era of exceedingly high loss of life due to HIV/AIDS. At the same time, many geographies saw life expectancy stagnate or decline, particularly for men and in countries with rising mortality from war or interpersonal violence. From 2005 to 2015, male life expectancy in Syria dropped by 11·3 years (3·7�17·4), to 62·6 years (56·5�70·2). Total deaths increased by 4·1 (2·6�5·6) from 2005 to 2015, rising to 55·8 million (54·9 million to 56·6 million) in 2015, but age-standardised death rates fell by 17·0 (15·8�18·1) during this time, underscoring changes in population growth and shifts in global age structures. The result was similar for non-communicable diseases (NCDs), with total deaths from these causes increasing by 14·1 (12·6�16·0) to 39·8 million (39·2 million to 40·5 million) in 2015, whereas age-standardised rates decreased by 13·1 (11·9�14·3). Globally, this mortality pattern emerged for several NCDs, including several types of cancer, ischaemic heart disease, cirrhosis, and Alzheimer's disease and other dementias. By contrast, both total deaths and age-standardised death rates due to communicable, maternal, neonatal, and nutritional conditions significantly declined from 2005 to 2015, gains largely attributable to decreases in mortality rates due to HIV/AIDS (42·1, 39·1�44·6), malaria (43·1, 34·7�51·8), neonatal preterm birth complications (29·8, 24·8�34·9), and maternal disorders (29·1, 19·3�37·1). Progress was slower for several causes, such as lower respiratory infections and nutritional deficiencies, whereas deaths increased for others, including dengue and drug use disorders. Age-standardised death rates due to injuries significantly declined from 2005 to 2015, yet interpersonal violence and war claimed increasingly more lives in some regions, particularly in the Middle East. In 2015, rotaviral enteritis (rotavirus) was the leading cause of under-5 deaths due to diarrhoea (146�000 deaths, 118�000�183�000) and pneumococcal pneumonia was the leading cause of under-5 deaths due to lower respiratory infections (393�000 deaths, 228�000�532�000), although pathogen-specific mortality varied by region. Globally, the effects of population growth, ageing, and changes in age-standardised death rates substantially differed by cause. Our analyses on the expected associations between cause-specific mortality and SDI show the regular shifts in cause of death composition and population age structure with rising SDI. Country patterns of premature mortality (measured as years of life lost YLLs) and how they differ from the level expected on the basis of SDI alone revealed distinct but highly heterogeneous patterns by region and country or territory. Ischaemic heart disease, stroke, and diabetes were among the leading causes of YLLs in most regions, but in many cases, intraregional results sharply diverged for ratios of observed and expected YLLs based on SDI. Communicable, maternal, neonatal, and nutritional diseases caused the most YLLs throughout sub-Saharan Africa, with observed YLLs far exceeding expected YLLs for countries in which malaria or HIV/AIDS remained the leading causes of early death. Interpretation At the global scale, age-specific mortality has steadily improved over the past 35 years; this pattern of general progress continued in the past decade. Progress has been faster in most countries than expected on the basis of development measured by the SDI. Against this background of progress, some countries have seen falls in life expectancy, and age-standardised death rates for some causes are increasing. Despite progress in reducing age-standardised death rates, population growth and ageing mean that the number of deaths from most non-communicable causes are increasing in most countries, putting increased demands on health systems. Funding Bill & Melinda Gates Foundation. © 2016 The Author(s). Published by Elsevier Ltd. This is an Open Access article under the CC BY licens

Лабораторний стенд з бездротовим інтерфейсом для дослідження систем автоматичного керування електроприводами постійного струму

Purpose. Development of a laboratory stand with a wireless interface for the study and research of automatic control systems for DC electric drives. Methodology. Physical experiment on the developed laboratory bench, computer modelling, calculation and analytical methods. Findings. The study considered and analyzed the advantages and disadvantages of existing developments of laboratory stands with virtual and remote components, the possibility of organizing a wireless interface, taking into account cost-effectiveness, mobility, reliability and simplicity, as well as the possibility of using as a training stand. The connection of the stand by means of USB and Wi-Fi is developed. The STM32F103C8T6 microcontroller is used for the power switch and the automatic control system. The interface part consists of a NodeMCU board, a MicroSD card module, an interface control unit, a 16x2 LCD and an I2C converter. The UART-USB interface is used for information transfer and programming of the stand. The possibility of current remote transmission of information about the modes and parameters of the engine to a computer with a browser output by installing the Wi-Fi module ESP8266MOD. A closed system of automatic DC motor control with PID current regulators and EMF has been developed. Experiments were performed with a pulse and smooth increase in motor speed and variation of the components of the PID controllers using the control panel of the laboratory stand. All graphs of the results of the experiment were obtained on a web page with a fixed IP address in the browser via Wi-Fi. Originality. The structure of the remote monitoring and control system based on hardware and software combination of telecommunication and measuring systems is proposed and developed, which differs from the existing ones by the presence of current wireless transmission of information, which allows to remotely receive research data of automatic DC motor control systems. Practical value. The developed laboratory stand with the wireless interface allows to receive and store experimental data on parameters of the investigated engine in real time remotely.Цель работы. Разработка лабораторного стенда с беспроводным интерфейсом для изучения и исследования систем автоматического управления электроприводами постоянного тока. Методы исследования. Физический эксперимент на разработанном лабораторном стенде, компьютерное моделирование, расчетно-аналитический методы. Полученные результаты. В ходе исследования были рассмотрены и проанализированы преимущества и недостатки существующих разработок лабораторных стендов с виртуальной и дистанционной составляющей, возможностью организации беспроводного интерфейса, с учетом экономичности, мобильности, надежности и простоты исполнения, а также возможности использования в качестве учебного стенда. Разработано подключение стенда с помощью USB и Wi-Fi. Для силового коммутатора и системы автоматического управления использован микроконтроллер STM32F103C8T6. Интерфейсная часть состоит из платы NodeMCU, модуля MicroSD карты, блока управления интерфейсом, LCD 16x2 и I2C преобразователя. Для передачи информации и программирования стенда применен интерфейс UART - USB. Предусмотрена возможность текущей дистанционной передачи информации о режимах и параметры работы двигателя к компьютеру с выводом на браузер путем установки модуля Wi-Fi ESP8266MOD. Разработана замкнутая система автоматического управления двигателем постоянного тока с ПИД-регуляторами тока и ЭДС. Были проведены эксперименты с импульсным и плавным увеличением скорости двигателя и варьированием составляющих ПИД-регуляторов с помощью пульта управления. Все графики результатов эксперимента были получены на веб-странице с фиксированным IP-адресом в браузере через Wi-Fi. Научная новизна. Предложена и разработана структура системы дистанционного мониторинга и управления на основе аппаратно-программного совмещения телекоммуникационной и измерительной систем, которая отличается от существующих наличием текущей беспроводной передачи информации, позволяет дистанционно получать данные исследований систем автоматического управления двигателем постоянного тока. Практическая ценность. Разработанный лабораторный стенд с беспроводным интерфейсом позволяет дистанционно получать и хранить экспериментальные данные о параметрах исследуемого двигателя в режиме реального времени.Мета роботи. Розробка лабораторного стенду з бездротовим інтерфейсом для вивчення та дослідження систем автоматичного керування електроприводами постійного струму. Методи дослідження. Фізичний експеримент на розробленому лабораторному стенді, комп’ютерне моделювання, розрахунково-аналітичний. Отримані результати. В ході дослідження було розглянуто та проаналізовано переваги та недоліки існуючих розробок лабораторних стендів з віртуальною та дистанційною складовою, можливістю організації бездротового інтерфейсу, з урахуванням економічності, мобільності, надійності і простоти виконання, а також можливості використання як навчального стенду. Розроблено підключення стенду за допомогою USB та Wi-Fi. Для силового комутатора та системи автоматичного керування використано мікроконтролер STM32F103C8T6. Інтерфейсна частина складається з плати NodeMCU, модуля MicroSD карти, блоку керування інтерфейсом, LCD 16x2 з I2C розширювачем. Передбачено можливість поточної дистанційної передачі інформації про режими та параметри роботи двигуна до комп’ютера з виводом на браузер через модуль Wi-Fi ESP8266MOD. Розроблено замкнену систему автоматичного керування двигуном постійного струму з ПІД-регуляторами струму та ЕРС. Було проведено експерименти з імпульсним та плавним збільшенням швидкості двигуна та варіюванням складових ПІД-регуляторів за допомогою пульта керування. Всі графіки результатів експерименту були отримані на веб-сторінці з фіксованою IP-адресою в браузері через Wi-Fi. Наукова новизна. Запропоновано та розроблено структуру системи дистанційного моніторингу та керування на основі апаратно-програмного суміщення телекомунікаційної та вимірювальної систем, яка відрізняється від існуючих наявністю поточної бездротової передачі інформації, що дозволяє дистанційно отримувати дані досліджень систем автоматичного керування двигуном постійного струму. Практична цінність. Розроблений лабораторний стенд з бездротовим інтерфейсом дозволяє дистанційно отримувати і зберігати експериментальні дані про параметри досліджуваного двигуна у режимі реального часу

The structure of interstitial lung diseases in children of the first two years of life

For the first time in Russia, the article provides data on interstitial lung diseases structure in children of the first two years of life, based on a series of observations of 68 patients with these rare diseases, as a part of multi-center ambispective study. Interstitial lung diseases in observed children included: Wilson-Mikity syndrome (23,4%), neuroendocrine hyperplasia of infancy (22%), bronchiolitis obliterans with organizing pneumonia (7,4%), primary pulmonary hypoplasia (1,5%), secondary pulmonary hypoplasia with Jeune syndrome (10,3%), secondary pulmonary hypoplasia with Edwards syndrome (2,9%), secondary pulmonary hypoplasia with other associated pathology (omphalocele - 1,5%, non-immune fetal hydrops - 1,5%), subpleural cysts in patients with Down syndrome (5,9%), congenital deficiency of surfactant protein B (1,5%), brain-lung- thyroid syndrome (2,9%), congenital alveolar-capillary dysplasia (1,5%), interstitial lung diseases with systemic diseases (Langerhans cell histiocytosis - 16,2%, Niemann-Pick disease - 1,5%). The article summarizes clinical features, the results of image diagnosis and disease outcomes. © 2015, Pediatria Ltd. All rights reserved