106 research outputs found
In vitro effects of regulators on growth and morphogenesis of Ocimum basilicum L. ‘Alfavaca Green’ stem apexes
Large-scale cultivation of contamination free plants requires a good standardization
protocol and production methods. Basil is widely used for cosmetics, food and pharmaceutical
industries as it is rich in many bioactive compounds. This present study aimed to evaluate the
growth and in vitro anatomical aspects of apical buds of basil grown under
different concentrations rowth regulators like: NAA (Naphthalenoacetic Acid), BAP
(6-benzylaminopurine), and KIN (Kinetin). The in vitro establishment was evaluated every 20
days to calculate the, the percentage of plants with calluses, appearance of the roots, any abnormal
seedlings, any oxidized seedlings, and the number of sprouts per plant. Growth, physiological,
and morpho-anatomical evaluations were performed at 80 days. Basal callogenesis was observed
when cytokinin’s and auxins are used in combination. Auxin treatments caused hyperhydricity in
the stems and leaves. Medium A2 (0.05 mg L
-1 of NAA and 0.1 mg L
-1 of BAP), and A3
(0.05 mg L
-1 of NAA and 0.1 mg L
-1 of KIN) resulted in the best development of basil plants,
cultivar ‘Alfavaca Green’. The A2 produced plants with greater numbers of leaves, an average
bud length of 59.81 mm, and the best root properties. A2 and A1 have a higher percentage of
hyperhydricity (83 and 67%). The A3 resulted in an acceptable number of leaves (range: 21–39),
and this treatment produced the best shoot properties as well as fewer plants with hyperhydricity.
In addition, the A3 treatment produced plants with a shoot length, high shoot fresh and dry mass
(2.82 and 0.23 g), high chlorophyll index and leaf anatomy that was similar to the control.
Excluding the control, the other treatments presented more than 90% of the explants with calluses
in their bases
Invasive fungal diseases in haematopoietic cell transplant recipients and in patients with acute myeloid leukaemia or myelodysplasia in Brazil
AbstractInvasive fungal disease (IFD) shows distinct regional incidence patterns and epidemiological features depending on the geographic region. We conducted a prospective survey in eight centres in Brazil from May 2007 to July 2009. All haematopoietic cell transplant (HCT) recipients and patients with acute myeloid leukaemia (AML) or myelodysplasia (MDS) were followed from admission until 1 year (HCT) or end of consolidation therapy (AML/MDS). The 12-month cumulative incidence (CI) of proven or probable IFD was calculated, and curves were compared using the Grey test. Among 237 AML/MDS patients and 700 HCT recipients (378 allogeneic, 322 autologous), the 1-year CI of IFD in AML/MDS, allogeneic HCT and autologous HCT was 18.7%, 11.3% and 1.9% (p <0.001), respectively. Fusariosis (23 episodes), aspergillosis (20 episodes) and candidiasis (11 episodes) were the most frequent IFD. The 1-year CI of aspergillosis and fusariosis in AML/MDS, allogeneic HCT and autologous HCT were 13.4%, 2.3% and 0% (p <0.001), and 5.2%, 3.8% and 0.6% (p 0.01), respectively. The 6-week probability of survival was 53%, and was lower in cases of fusariosis (41%). We observed a high burden of IFD and a high incidence and mortality for fusariosis in this first multicentre epidemiological study of IFD in haematological patients in Brazil
Accuracy of the estimates of ammonia concentration in rumen fluid using different analytical methods
Human toxocariasis: contribution by Brazilian researchers
In the present paper the main aspects of the natural history of human infection by Toxocara larvae that occasionally result in the occurrence of visceral and/or ocular larva migrans syndrome were reviewed. The contribution by Brazilian researchers was emphasized, especially the staff of the Tropical Medicine Institute of São Paulo (IMT)
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