Comportamento e bem-estar de peixe beta (Betta splendens) em aquário.

O trabalho será realizado no Instituto Federal de Educação, Ciência e Tecnologia do Espírito Santo/Campus Alegre, no laboratório de Nutrição e Produção de Espécies Ornamentais, localizado no município de Alegre, região Sul do estado do Espírito Santo, Brasil. Serão utilizados 70 exemplares machos de peixe Beta (Betta splendens) com idade entre 90 e 120 dias. Dos quais 15 exemplares de peixe Beta, machos adultos, com aproximadamente 90 dias serão manejados em aquário tipo cruzeta com quatro pontas de mesmo comprimento e distância do centro. Quatro testes serão realizados para analisar a preferência dos peixes quanto a tipo de (T1) substrato, de (T2) vegetações, de (T3) abrigos e de (T4) cores. Em cada teste será oferecido quatro tipos de preferências (P1, P2, P3 e P4) distribuídos aleatoriamente por sorteio. Serão analisados os comportamentos de frequência em cada preferência, duração em cada preferência e tempo de latência. No segundo trabalho serão utilizados 35 exemplares machos de peixe Beta com aproximadamente 90 dias distribuídos aleatoriamente em cinco tipos de alojamento (tratamentos); (T1) copos de 0,3 litros, (T2) beteiras 3 litros sem enriquecimento, (T3) beteiras 3 litros enriquecidas, (T4) aquários 38 litros sem enriquecimento e (T5) aquários 38 litros enriquecidos. Os comportamentos dos animais serão gravados durante todo o período experimental. Será realizado um total de dezesseis horas de observações para cada tratamento. Durante o mesmo período também será realizado observação focal durante cinco minutos intermitentes alternando entre os tratamentos no total de 150 minutos por período. Os comportamentos registrados serão organizados formando um etograma e comparados entre os diferentes tratamentos. No último trabalho serão utilizados os 35 exemplares machos de peixe Beta, advindos do experimento de enriquecimento, com aproximadamente 120 dias em aquário tipo labirinto. Localizado dentro do labirinto haverá quatro pontos; dois objetos estressores, espelho (E1) e predador (E2), e dois objetos recompensa ambiente enriquecido (R1) e alimento (R2). Comportamentos como lateralidade, latência, preferência, frequência e duração serão analisados durante 15 minutos na parta da manhã e 15 minutos na parte da tarde durante dois dias consecutivos. Então os animais terão cinco dias de descanso e retornarão ao labirinto para mais 15 minutos de observações comportamentais na parta da manhã e 15 minutos na parte da tarde durante dois dias consecutivos

The founding charter of the Genomic Observatories Network

The co-authors of this paper hereby state their intention to work together to launch the Genomic Observatories Network (GOs Network) for which this document will serve as its Founding Charter. We define a Genomic Observatory as an ecosystem and/or site subject to long-term scientific research, including (but not limited to) the sustained study of genomic biodiversity from single-celled microbes to multicellular organisms.An international group of 64 scientists first published the call for a global network of Genomic Observatories in January 2012. The vision for such a network was expanded in a subsequent paper and developed over a series of meetings in Bremen (Germany), Shenzhen (China), Moorea (French Polynesia), Oxford (UK), Pacific Grove (California, USA), Washington (DC, USA), and London (UK). While this community-building process continues, here we express our mutual intent to establish the GOs Network formally, and to describe our shared vision for its future. The views expressed here are ours alone as individual scientists, and do not necessarily represent those of the institutions with which we are affiliated.Neil Davies ... Andrew J Lowe ... et al. and GOs-CO

Glial reaction after pyramidotomy in mice and rats

10.1006/neur.1995.0049Neurodegeneration44403-413NEUR

The Occurrence and Effect of a Protozoan Parasite, Ophryocystis elektroscirrha (Neogregarinida: Ophryocystidae) on Overwintering Monarch Butterflies, Danaus Plexippus (Lepidoptera: Danaidae) from Two California Winter Sites

1. Monarch butterflies, Danaus plexippus, from two overwintering populations, were found to have Ophryocystis elektroscirrha spores on their scales at rates between 53% and 68%. The frequency of butterflies with O.elektroscirrhaspores remained about the same between sites and throughout the winter. 2. The spores, recovered from all parts of the body of the butterfly, were most numerous on the abdomen, particularly near the posterior third. 3. Butterflies with spores survived as long as those without detectable spores at 10.1°C ±0.4 SE and 78.3% r.h. ±0.6SE. Insects with spores held at 19.4°C ±0.4SE and 44.9% r.h. ±1.5SE showed a significantly higher rate of moisture loss and survived a shorter period than monarch butterflies without detectable spores

Differentiation of bean-infecting geminiviruses by nucleic acid hybridization probes and aspects of bean golden mosaic in Brazil

A bean golden mosaic geminiviral isolate from Goiania, Goiás, Brazil (BGMV-BZ), was determined to be transmitted by whiteflies and induced golden mosaic symptoms and diagnostic geminiviral inclusion bodies and ultrastructural abnormalities in infected bean leaves. In contrast to BGMV isolates from Central America and the Caribbean, BGMV-BZ could not be sap-transmitted to beans. Cloned geminiviral DNA components were used as DNA probes for the rapid and specific detection of BGMV-BZ and three sap-transmissible bean-infecting geminiviral isolates—BGMV from Guatemala (BGMV-GA) and the Dominican Republic (BGMV-DR) and bean dwarf mosaic geminivirus from Colombia (BDMV-CO). A general DNA probe detected all four viral isolates, whereas specific probes detected BGMV-BZ, BDMV, or BGMV-GA and BGMV-DR. Nucleic acid dot and squash blot methods were used to prepare samples for hybridization, and the dot blot method was used to determine relative differences in viral nucleic acid titers in infected bean leaves. The general and specific probes were employed to study the variability of BGMV isolates and potential weed reservoirs of geminiviruses in the Dominican Republic

Differential response of LV sublayer twist during dobutamine stress echocardiography as a novel marker of contractile reserve after acute myocardial infarction: relationship with follow-up LVEF improvement

Cardiolog

Nutlin-3 sensitizes nasopharyngeal carcinoma cells to cisplatin-induced cytotoxicity

The small-molecule inhibitor of p53-Mdm2 interaction, Nutlin-3, is known to be effective against cancers expressing wild-type (wt) p53. p53 mutations are rare in nasopharyngeal carcinoma (NPC), hence targeting disruption of p53-Mdm2 interaction to reactivate p53 may offer a promising therapeutic strategy for NPC. In the present study, the effects of Nutlin-3 alone or in combination with cisplatin, a standard chemotherapeutic agent, were tested on C666-1 cells, an Epstein-Barr virus (EBV)-positive NPC cell line bearing wt p53. Treatment with Nutlin-3 activated the p53 pathway and sensitized NPC cells to the cytotoxic effects of cisplatin. The combined treatment also markedly suppressed soft agar colony growth formation and increased apoptosis of NPC cells. The effect of Nutlin-3 on NPC cells was inhibited by knockdown of p53, suggesting that its effect was p53-dependent. Extended treatment with increasing concentrations of Nutlin-3 did not result in emergence of p53 mutations in the C666-1 cells. Collectively, the present study revealed supportive evidence of the effectiveness of combining cisplatin and Nutlin-3 as a potential therapy against NPC

Left ventricular dyssynchrony induced by chronic right ventricular pacing is associated with long-term poor outcome

Cardiolog