Lower hybrid current drive at high density in Alcator C-Mod

Experimental observations of lower hybrid current drive (LHCD) at high density on the Alcator C-Mod tokamak are presented in this paper. Bremsstrahlung emission from relativistic fast electrons in the core plasma drops suddenly above line-averaged densities of 10[superscript 20] m[superscript −3] (ω/ω[subscript LH] ~ 3) in single null discharges with large (≥8 mm) inner gaps, well below the density limit previously observed on limited tokamaks (ω/ω[subscript LH] ~ 2). Modelling and experimental evidence suggest that the absence of LHCD driven fast electrons at high density may be due to parasitic collisional absorption in the scrape-off layer (SOL). Experiments show that the population of fast electrons produced by LHCD at high density ([bar over n][subscript e] > 10[superscript 20] m[superscript -3]) can be increased by operating with an inner gap of less than ~5 mm with the strongest non-thermal emission in inner wall limited plasmas. A change in plasma topology from single to double null produces a modest increase in non-thermal emission at high density. Increasing the electron temperature in the periphery of the plasma (0.8 > r/a > 1.0) also results in a modest increase in non-thermal electron emission above the density limit. Ray tracing/Fokker–Planck simulations of these discharges predict the observed sensitivity to plasma position when the effects of collisional absorption in the SOL are included in the model.United States. Dept. of Energy (Award DE-FC02-99ER54512)United States. Dept. of Energy (Award DE-AC02-76CH03073

Novel insights into the early stages of infection by oval conidia of Colletotrichum sublineolum

Anthracnose, caused by Colletotrichum sublineolum Henn. ex Sacc. & Trotter, is one of the most important sorghum [Sorghum bicolor (L.) Moench] diseases in Brazil. This fungus exhibits conidial dimorphism, producing either falcate or oval conidia on solid and liquid media, respectively. We compared patterns of the initial infection events by these two types of conidia on sorghum leaves using light microscopy and scanning electron microscopy. The infection events during the first 24 h were similar for both oval and falcate conidia. Globose and melanized apressoria were formed at 24 h after inoculation (hai) regardless of the conidia type. Dense mycelium and oval conidia developed from germinated falcate conidia at 32 hai. Hyphal mass displaying acervuli filled with falcate conidia and surrounded by setae, developed from germinated oval conidia at 48 hai. Oval conidia were as capable as falcate conidia of infecting sorghum leaves. The inherent ability to grow faster and the easeness with which oval conidia can be produced in vitro as compared to falcate, make the former a preferred choice for studies on the C. sublineolum-sorghum interaction. It would be instructive to further investigate the potential role of the oval conidia in epidemics

Lifestyle transitions in plant pathogenic Colletotrichum fungi deciphered by genome and transcriptome analyses

Colletotrichum species are fungal pathogens that devastate crop plants worldwide. Host infection involves the differentiation of specialized cell types that are associated with penetration, growth inside living host cells (biotrophy) and tissue destruction (necrotrophy). We report here genome and transcriptome analyses of Colletotrichum higginsianum infecting Arabidopsis thaliana and Colletotrichum graminicola infecting maize. Comparative genomics showed that both fungi have large sets of pathogenicity-related genes, but families of genes encoding secreted effectors, pectin-degrading enzymes, secondary metabolism enzymes, transporters and peptidases are expanded in C. higginsianum. Genome-wide expression profiling revealed that these genes are transcribed in successive waves that are linked to pathogenic transitions: effectors and secondary metabolism enzymes are induced before penetration and during biotrophy, whereas most hydrolases and transporters are upregulated later, at the switch to necrotrophy. Our findings show that preinvasion perception of plant-derived signals substantially reprograms fungal gene expression and indicate previously unknown functions for particular fungal cell types