1,531 research outputs found
Acquisition of an Agrobacterium Ri Plasmid and Pathogenicity by Other -Proteobacteria in Cucumber and Tomato Crops Affected by Root Mat
Root mat of cucumbers and tomatoes has previously been shown to be caused by Agrobacterium radiobacter strains harboring a root-inducing Ri plasmid (pRi). Nine other pRi-harboring -Proteobacteria have subsequently been isolated from root mat-infected crops. Fatty acid profiling and partial 16S rRNA sequence analysis identified three of these strains as being in the genus Ochrobactrum, five as being in the genus Rhizobium, and one as being in the genus Sinorhizobium. An in vitro pathogenicity test involving inoculation of cucumber cotyledons was developed. All pRi-harboring -Proteobacteria induced typical root mat symptoms from the cotyledons. Average transformation rates for rhizogenic Ochrobactrum (46%) and Rhizobium (44%) strains were lower than those observed for rhizogenic A. radiobacter strains (64%). However, individual strains from these three genera all had transformation rates comparable to those observed from cotyledons inoculatedwith a rhizogenic Sinorhizobium strain (75%)
EIS/Hinode observations of Doppler flow seen through the 40 arcsec wide slit
The Extreme ultraviolet Imaging Spectrometer (EIS) on board Hinode is the
first solar telescope to obtain wide slit spectral images that can be used for
detecting Doppler flows in transition region and coronal lines on the Sun and
to relate them to their surrounding small scale dynamics. We select EIS lines
covering the temperature range 6x10^4 K to 2x10^6 K that give spectrally pure
images of the Sun with the 40 arcsec slit. In these images Doppler shifts are
seen as horizontal brightenings. Inside the image it is difficult to
distinguish shifts from horizontal structures but emission beyond the image
edge can be unambiguously identified as a line shift in several lines separated
from others on their blue or red side by more than the width of the
spectrometer slit (40 pixels). In the blue wing of He II, we find a large
number of events with properties (size and lifetime) similar to the
well-studied explosive events seen in the ultraviolet spectral range.
Comparison with X-Ray Telescope (XRT) images shows many Doppler shift events at
the footpoints of small X-ray loops. The most spectacular event observed showed
a strong blue shift in transition region and lower corona lines from a small
X-ray spot that lasted less than 7 min. The emission appears to be near a cool
coronal loop connecting an X-ray bright point to an adjacent region of quiet
Sun. The width of the emission implies a line-of-sight velocity of 220 km/s. In
addition, we show an example of an Fe XV shift with a velocity about 120 km/s,
coming from what looks like a narrow loop leg connecting a small X-ray
brightening to a larger region of X-ray emission.Comment: 12 pages, 8 figures, to be published in Solar Physic
Radio Source Heating in the ICM: The Example of Cygnus A
One of the most promising solutions for the cooling flow problem involves
energy injection from the central AGN. However it is still not clear how
collimated jets can heat the ICM at large scale, and very little is known
concerning the effect of radio lobe expansion as they enter into pressure
equilibrium with the surrounding cluster gas. Cygnus A is one of the best
examples of a nearby powerful radio galaxy for which the synchrotron emitting
plasma and thermal emitting intra-cluster medium can be mapped in fine detail,
and previous observations have inferred possible shock structure at the
location of the cocoon. We use new XMM-Newton observations of Cygnus A, in
combination with deep Chandra observations, to measure the temperature of the
intra-cluster medium around the expanding radio cavities. We investigate how
inflation of the cavities may relate to shock heating of the intra-cluster gas,
and whether such a mechanism is sufficient to provide enough energy to offset
cooling to the extent observed.Comment: To appear in the Proceedings of "Heating vs. Cooling in Galaxies and
Clusters of Galaxies", August 2006, Garching (Germany), Eds. H. Boehringer,
G.W. Pratt, A. Finoguenov, P. Schuecker, Springer-Verlag series "ESO
Astrophysics Symposia", p.101, in press. 8 pages, 3 multiple figure
High Energy Gamma Ray Production from Proton Induced Reactions on D, C, Zn, Pb at Incident Energies of 104, 145, and 195 MeV
This research was sponsored by the National Science Foundation Grant NSF PHY-931478
Understanding person acquisition using an interactive activation and competition network
Face perception is one of the most developed visual skills that humans display, and recent work has attempted to examine the mechanisms involved in face perception through noting how neural networks achieve the same performance. The purpose of the present paper is to extend this approach to look not just at human face recognition, but also at human face acquisition. Experiment 1 presents empirical data to describe the acquisition over time of appropriate representations for newly encountered faces. These results are compared with those of Simulation 1, in which a modified IAC network capable of modelling the acquisition process is generated. Experiment 2 and Simulation 2 explore the mechanisms of learning further, and it is demonstrated that the acquisition of a set of associated new facts is easier than the acquisition of individual facts in isolation of one another. This is explained in terms of the advantage gained from additional inputs and mutual reinforcement of developing links within an interactive neural network system. <br/
muSR in Ce_{1-x}La_xAl_3: anisotropic Kondo effect?
Zero-field muSR experiments in the heavy-fermion alloys Ce_{1-x}La_xAl_3, x =
0 and 0.2, examine a recent proposal that the system exhibits a strong
anisotropic Kondo effect. We resolve a damped oscillatory component for both La
concentrations, indicative of disordered antiferromagnetism. For x = 0.2 the
oscillation frequency decreases smoothly with increasing temperature, and
vanishes at the specific heat anomaly temperature T* \approx 2.2 K. Our results
are consistent with the view that T* is due to a magnetic transition rather
than anisotropic Kondo behavior.Comment: 5 pages, 2 figures. To be published in proceedings of musr2002
(Physica B
Smectic ordering in liquid crystal - aerosil dispersions I. X-ray scattering
Comprehensive x-ray scattering studies have characterized the smectic
ordering of octylcyanobiphenyl (8CB) confined in the hydrogen-bonded silica
gels formed by aerosil dispersions. For all densities of aerosil and all
measurement temperatures, the correlations remain short range, demonstrating
that the disorder imposed by the gels destroys the nematic (N) to smectic-A
(SmA) transition. The smectic correlation function contains two distinct
contributions. The first has a form identical to that describing the critical
thermal fluctuations in pure 8CB near the N-SmA transition, and this term
displays a temperature dependence at high temperatures similar to that of the
pure liquid crystal. The second term, which is negligible at high temperatures
but dominates at low temperatures, has a shape given by the thermal term
squared and describes the static fluctuations due to random fields induced by
confinement in the gel. The correlation lengths appearing in the thermal and
disorder terms are the same and show strong variation with gel density at low
temperatures. The temperature dependence of the amplitude of the static
fluctuations further suggests that nematic susceptibility become suppressed
with increasing quenched disorder. The results overall are well described by a
mapping of the liquid crystal-aerosil system into a three dimensional XY model
in a random field with disorder strength varying linearly with the aerosil
density.Comment: 14 pages, 13 figure
Rapid conjugation of antibodies to toxins to select candidates for the development of anticancer Antibody-Drug conjugates (ADcs)
Antibody-Drug Conjugates (ADCs) developed as a targeted treatment approach to deliver toxins directly to cancer cells are one of the fastest growing classes of oncology therapeutics, with eight ADCs and two immunotoxins approved for clinical use. However, selection of an optimum target and payload combination, to achieve maximal therapeutic efficacy without excessive toxicity, presents a significant challenge. We have developed a platform to facilitate rapid and cost-effective screening of antibody and toxin combinations for activity and safety, based on streptavidin-biotin conjugation. For antibody selection, we evaluated internalization by target cells using streptavidin-linked antibodies conjugated to biotinylated saporin, a toxin unable to cross cell membranes. For payload selection, we biotinylated toxins and conjugated them to antibodies linked to streptavidin to evaluate antitumour activity and pre-clinical safety. As proof of principle, we compared trastuzumab conjugated to emtansine via streptavidin-biotin (Trastuzumab-SB-DM1) to the clinically approved trastuzumab emtansine (T-DM1). We showed comparable potency in reduction of breast cancer cell survival in vitro and in growth restriction of orthotopic breast cancer xenografts in vivo. Our findings indicate efficient generation of functionally active ADCs. This approach can facilitate the study of antibody and payload combinations for selection of promising candidates for future ADC development
Sources and contributions of wood smoke during winter in London: Assessing local and regional influences
Determining the contribution of wood smoke to air pollution in large cities such as London is becoming increasingly important due to the changing nature of domestic heating in urban areas. During winter, biomass burning emissions have been identified as a major cause of exceedances of European air quality limits. The aim of this work was to quantify the contribution of biomass burning in London to concentrations of PM2:5 and determine whether local emissions or regional contributions were the main source of biomass smoke. To achieve this, a number of biomass burning chemical tracers were analysed at a site within central London and two sites in surrounding rural areas. Concentrations of levoglucosan, elemental carbon (EC), organic carbon (OC) and KC were generally well correlated across the three sites. At all the sites, biomass burning was found to be a source of OC and EC, with the largest contribution of EC from traffic emissions, while for OC the dominant fraction included contributions from secondary organic aerosols, primary biogenic and cooking sources. Source apportionment of the EC and OC was found to give reasonable estimation of the total carbon from non-fossil and fossil fuel sources based upon comparison with estimates derived from 14C analysis. Aethalometer-derived black carbon data were also apportioned into the contributions frombiomass burning and traffic and showed trends similar to those observed for EC. Mean wood smoke mass at the sites was estimated to range from 0.78 to 1.0 μgm-3 during the campaign in January–February 2012. Measurements on a 160m tower in London suggested a similar ratio of brown to black carbon (reflecting wood burning and traffic respectively) in regional and London air. Peaks in the levoglucosan and KC concentrations were observed to coincide with low ambient temperature, consistent with domestic heating as a major contributing local source in London. Overall, the source of biomass smoke in London was concluded to be a background regional source overlaid by contributions from local domestic burning emissions. This could have implications when considering future emission control strategies during winter and may be the focus of future work in order to better determine the contributing local sources
The Kuiper Belt and Other Debris Disks
We discuss the current knowledge of the Solar system, focusing on bodies in
the outer regions, on the information they provide concerning Solar system
formation, and on the possible relationships that may exist between our system
and the debris disks of other stars. Beyond the domains of the Terrestrial and
giant planets, the comets in the Kuiper belt and the Oort cloud preserve some
of our most pristine materials. The Kuiper belt, in particular, is a
collisional dust source and a scientific bridge to the dusty "debris disks"
observed around many nearby main-sequence stars. Study of the Solar system
provides a level of detail that we cannot discern in the distant disks while
observations of the disks may help to set the Solar system in proper context.Comment: 50 pages, 25 Figures. To appear in conference proceedings book
"Astrophysics in the Next Decade
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