Nitrogen And Potassium Fertilization In A Guava Orchard Evaluated For Five Cycles: Effects On The Plant And On Production

Guava response to fertilization can be monitored through plant tissue analysis. Correct interpretation of these results, based on standard levels, is of great importance for correct nutrient management of the crop. However, standard levels are constantly criticized for not considering interactions among elements. To improve the nutritional diagnosis of ‘Paluma’ guava (Psidium guajava L., Myrtaceae), an experiment was conducted using nitrogen fertilization (0, 0.5, 1.0, and 2.0 kg per plant per cycle of N, with urea as a source with 45 % N), and potassium fertilization (0, 0.55, 1.1, and 2.2 kg per plant per cycle of K2O, with potassium chloride as a source with 60 % K2O) in an irrigated commercial area for five consecutive cycles, 2009 through 2012, observing the influence of fertilizers and climate and assessing yield and leaf element content, using the concept of isometric log ratios (ilr) to interpret leaf analysis results (N, P, Ca, Mg, K, and S). This paper showed that nutrient balances and nutrient concentration values can be interpreted coherently using compositional data analysis. Ranges of nutrient balances also were established for “Paluma” guava and validated through ranges grounded in nutrient contents currently used in Brazil. Nitrogen fertilization increased “Paluma” guava yield. The 0.5 kg N application rate per plant and the other studied treatments practically showed the same results, and their values were affected by pruning time as well as the nutrient balances. © 2016, Revista Brasileira de Ciencia do Solo. All rights reserved.4

Nitrogen And Potassium Fertilization In A Guava Orchard Evaluated For Five Cycles: Soil Cationic Balance

Soil fertility evaluation through soil analysis traditionally does not consider interaction among elements. To include the interaction effect in interpretation of soil analyses, an experiment was conducted to evaluate the effect of nitrogen fertilization (0, 0.5, 1.0, and 2.0 kg N per plant per cycle) with urea (45% N) and potassium fertilization (0, 0.55, 1.1, and 2.2 kg K2O per plant per cycle) with potassium chloride (60% K2O) on soil cationic balance. The experiment was carried out in an irrigated commercial production area of ‘Paluma’ guava, for five consecutive cycles, 2009 through 2012, using the concept of isometric log ratio (ilr) to evaluate soil cationic balance through [K, Ca, Mg | H+Al], [K | Ca, Mg] and [Ca | Mg] balances. The compositional data analysis showed to be a suitable tool to interpret the soil cationic balance given that the soil cationic balances value was changed by nitrogen fertilization and potassium as well as the soil pH value and the concentration of K in the soil. The soil cationic balances also changed by the variations of in climate conditions at period of soil sample. An application rate of 0.55 kg K2O per plant per cycle was considered sufficient to keep K soil concentration above 1.6 mmolc dm-3. © 2016, Revista Brasileira de Ciencia do Solo. All rights reserved.4

Perspectives on tracking data reuse across biodata resources.

Data reuse is a common and vital practice in molecular biology and enables the knowledge gathered over recent decades to drive discovery and innovation in the life sciences. Much of this knowledge has been collated into molecular biology databases, such as UniProtKB, and these resources derive enormous value from sharing data among themselves. However, quantifying and documenting this kind of data reuse remains a challenge. The article reports on a one-day virtual workshop hosted by the UniProt Consortium in March 2023, attended by representatives from biodata resources, experts in data management, and NIH program managers. Workshop discussions focused on strategies for tracking data reuse, best practices for reusing data, and the challenges associated with data reuse and tracking. Surveys and discussions showed that data reuse is widespread, but critical information for reproducibility is sometimes lacking. Challenges include costs of tracking data reuse, tensions between tracking data and open sharing, restrictive licenses, and difficulties in tracking commercial data use. Recommendations that emerged from the discussion include: development of standardized formats for documenting data reuse, education about the obstacles posed by restrictive licenses, and continued recognition by funding agencies that data management is a critical activity that requires dedicated resources. Summaries of survey results are available at: https://docs.google.com/forms/d/1j-VU2ifEKb9C-sW6l3ATB79dgHdRk5v_lESv2hawnso/viewanalytics (survey of data providers) and https://docs.google.com/forms/d/18WbJFutUd7qiZoEzbOytFYXSfWFT61hVce0vjvIwIjk/viewanalytics (survey of users)

Using hyperosmolar stress to measure biologic and stress-activated protein kinase responses in preimplantation embryos

We used hyperosmolar stress to test blastocysts for their biologic and enzymatic responses to culture stress. Embryos mount dose- and time-dependent responses to hyperosmolar stress. Biological responses included slowed cavitation and cell accumulation and increased apoptosis at increasing doses. These responses were preceded by stress-activated protein kinase (SAPK) phosphorylation and nuclear translocation consistent with its causal role. For cavitation and new cell cycle initiation, 200 mM sorbitol caused stasis. Above 200 mM, sorbitol was ultimately lethal and below 200 mM, its embryos had milder effects. Phosphorylated SAPK was induced rapidly in embryos at 0.5 h in a dose-dependent manner from 0 to 600 mM sorbitol. Higher hyperosmolarity caused a biphasic peak of phosphorylated SAPK, but there was no return to baseline through 3 h. At 24 h, a dose-dependent response persisted that was linear from 0 to 200 mM sorbitol. Hyperosmolar stress rapidly induced, within 0.5 h, phosphorylated, nuclear c-Jun and decreased phosphorylated, nuclear c-Myc in a SAPK-dependent manner. The data suggest that SAPK is induced and functions on down-stream effector molecules in a temporal and quantitative manner consistent with its function in the embryonic homeostatic response to stress. The remarkable resistance of embryos to high concentrations of sorbitol suggests that part of its homeostatic response is different from that of somatic cells

The InterPro protein families database: the classification resource after 15 years.

The InterPro database (http://www.ebi.ac.uk/interpro/) is a freely available resource that can be used to classify sequences into protein families and to predict the presence of important domains and sites. Central to the InterPro database are predictive models, known as signatures, from a range of different protein family databases that have different biological focuses and use different methodological approaches to classify protein families and domains. InterPro integrates these signatures, capitalizing on the respective strengths of the individual databases, to produce a powerful protein classification resource. Here, we report on the status of InterPro as it enters its 15th year of operation, and give an overview of new developments with the database and its associated Web interfaces and software. In particular, the new domain architecture search tool is described and the process of mapping of Gene Ontology terms to InterPro is outlined. We also discuss the challenges faced by the resource given the explosive growth in sequence data in recent years. InterPro (version 48.0) contains 36 766 member database signatures integrated into 26 238 InterPro entries, an increase of over 3993 entries (5081 signatures), since 2012

Two-Loop O(alpha_s G_F M_Q^2) Heavy-Quark Corrections to the Interactions between Higgs and Intermediate Bosons

By means of a low-energy theorem, we analyze at O(alpha_s G_F M_Q^2) the shifts in the Standard-Model W^+W^-H and ZZH couplings induced by virtual high-mass quarks, Q, with M_Q >> M_Z, M_H, which includes the top quark. Invoking the improved Born approximation, we then find the corresponding corrections to various four- and five-point Higgs-boson production and decay processes which involve the W^+W^-H and ZZH vertices with one or both of the gauge bosons being connected to light-fermion currents, respectively. This includes e^+e^- -> f anti-f H via Higgs-strahlung, via W^+W^- fusion (with f = nu_e), and via ZZ fusion (with f = e), as well as H -> 2V -> 4f (with V = W, Z).Comment: 20 pages (Latex); Physical Review D (to appear

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries