Contribuição dos humanos, cães e gatos à transmissão do Trypanosoma cruzi na Região do Chaco Argentino

Foi determinada a prevalência da infecção por T. cruzi nos humanos, cães e gatos, pertencentes a 47 rancherías em três povoados rurais; Guanaco Muerto (Córdoba), La Invernada e Amamás (Santiago del Estero), mediante reações seroldgicas e xenodiagnóstico. Poram examinadas 245 pessoas, 123 cães e 14 gatos. A taxa de prevalência na população foi entre 58,7% (GM) e 49,6% (LI). Foram detectados 76% de cães infectados, o que resultou significativamente superior aos 51% encontrados nos humanos. As porcentagens de cães (64,2%) e gatos (63,6%) com parasitemia foram significativamente superiores à correspondente aos humanos (12,5%). Se bem que 79% dos gatos estavam infectados, sua pequena quantidade e seus hábitos de perambulação determinam que sua participação na transmissão doméstica do T. cruzi seja restrita. Não obstante existir em média um maior número de humanos que de cães em cada lar, tanto de sujeitos sãos como infectados (6,5 vs. 3,3 e 3,4 vs. 2,4, respectivamente), foram detectados também na média mais cães que humanos com parasitemia em cada casa (2,1 vs. 1,0). As altas porcentagens de cães infectados e com parasitemia, além do hábito de repouso intra-domiciliário o qual ocasiona estreito contacto entre eles e os barbeiros determinam que os cães sejam os principais provedores de parásitos à disposição para a transmissão, e os hospedeiros mais importantes para /nanutenção da doença de Chagas na Região do Chaco Argentino.Trypanosoma cruzi prevalence rates of human, dog and cat populations from 47 households of 3 rural localities of the phytogeographical Chaqueña area of Argentina were determined both by serological and xenodiagnostic procedures. Human prevalence rates were uniform and ranged from 49.6 to 58.7%. Overall prevalence rate in dogs (75.0%) was significantly higher than in humans (51.0%). The overall proportion of parasitemic individuals assessed by xenodiagnosis was significantly higher in either dog (64.2%) or cat (63.6%) populations than among humans (12.5%). Although both the average number of resident as well as infected individuals per household was higher for people than for dogs (6.5 vs. 3.3, and 3.4 vs. 2.4, respectively), the reverse was recorded when parasitemic individuals were considered (1.0 vs. 2.1). Results are discussed in relation to dog between dogs and people, and dogs and bugs. In the light of present data, dogs must be considered as the major donors of parasites to vector bugs and thus, principal contributors to transmission in this region of Argentina

Dinâmica da transmissão do Trypanosoma cruzi numa área rural da Argentina: III. Persistência da parasitemia pelo T. cruzi em reservatórios caninos durante um seguimento de dois anos

A new cross-sectional survey of household- associated mongrel dogs as well as follow-up of previously parasitemic individuals was carried out in 1984 toy means of xenodiagnosis and serologic techniques to get a deeper insight into the relationship of T. cruzi parasitemia and age among canine hosts in a rural area of Argentina. Persistence of detectable parasitemia was age-independent, or at most, loosely related to age, confirming the pattern observed in 1982. Similarly no significant age-decreasing effect was recorded among seropositive dogs in: a) the probability of detecting parasites in a 2-year follow-up; b) their intensity of infectiousness (=infective force) for T. infestans 3rd-4th instar nymphs, as measured by the percentage of infected bugs observed in each dog xenodiagnosis. Moreover, not only was the infective force of seropositive dogs for bugs approximately constant through lifetime, but it was significantly higher than the one recorded for children in the present survey, and for human people by other researchers. Therefore, and since T. infestans field populations show high feeding frequencies on dogs, the latter are expected to make the greatest contribution to the pool of infected vectors in the rural household of Argentina. This characteristic should be sufficient to involve canine reservoirs definitely as a risk factor for human people residing in the same house. The increased severity of parasitemia observed among dogs in this survey may be related to the acute undernutrition characteristic of canine populations of poor rural areas in our country, which is expected to affect the ability of the host to manage the infection.Foi realizada em 1984 uma nova pesquisa epidemiológica dos cães domésticos e seguimento dos indivíduos que apresentaram parasitemia dois anos antes a fim de compreender a relação entre a parasitemia e a idade dos cães, empregando para isso técnicas sorológicas e xenodiagnóstico. A persistência da parasitemia foi independente ou pouco relacionada à idade, confirmando assim o padrão observado em 1982. Da mesma maneira, nos cães soropositivos não se registrou nenhuma diminuição significativa com respeito a idade em: a) a probabilidade de descobrir parasitas por meio de um seguimento durante 2 anos; b) sua capacidade de infecção para as ninfas de 3.ª 4.ª fase de T. infestans, medido pela porcentagem de barbeiros infectados que se observou em cada um dos xenodiagnósticos dos cães. A capacidade infectante dos cães soropositivos foi aproximadamente constante ao longo da vida, e significativamente maior que a registrada para as crian. ças no presente estudo, e para os agrupamentos humanos pesquisados por outros investigadores. Assim sendo e considerando as altas freqüências de alimentação no cão que são observados nas populações pelo T. infestans, espera-se que o cão contribua para o total de barbeiros infectados existentes nas áreas rurais da Argentina. Esta característica deveria ser suficiente para incriminar definitivamente os reservatórios caninos como um fator de risco para as pessoas residentes na mesma casa. A alta intensidade da parasitemia registrada entre os cães na presente pesquisa poderia estar relacionada com a acentuada desnutrição característica dos cães nas áreas rurais pobres da Argentina, o que poderia afetar a capacidade do hospedeiro para controlar a infecção

Fasciolosis en bovinos hembras en crecimiento del Departamento Berón de Astrada (Corrientes, Argentina)

El objetivo del trabajo fue estudiar la evolución de la fasciolosis bovina a largo plazo, a partir hembras en crecimiento al pie de madre y hasta los 3 años de edad, efectuando el diagnóstico in vivo por coprología y evaluando la respuesta al tratamiento instaurado (albendazole). Las tareas de campo se realizaron en un establecimiento del Departamento Berón de Astrada (Corrientes, Argentina), zona donde la infestación del ganado con Fasciola hepatica es enzoótica. El grupo investigado estuvo inicialmente constituido por 78 animales, los que al final del ensayo se redujeron a 30. Se realizaron seis muestreos de materia fecal para estudios coprológicos por sedimentación (Dennis), los tres primeros coincidentes con los tres tratamientos antiparasitarios efectuados. El día 0 se verificó un 3,85% de animales positivos. La tasa fue de 1,30% al día 71 y de 0% durante los dos muestreos sucesivos, a los 172 y 230 días, avalando la eficacia de los tratamientos recibidos. La interrupción de la administración del antiparasitario provocó que la “fasciolosis” reapareciera al día 553 con 7,69% de animales positivos, nivel que ascendió al 80% el día 850 (ultimo muestreo). Se concluye alertando sobre los efectos contraproducentes de la práctica habitual de interrumpir los tratamientos antiparasitarios contra F. hepatica en animales adultos, porque ellos son losgrandes diseminadores de esta enfermedad parasitaria

The Sagnac Effect in curved space-times from an analogy with the Aharonov-Bohm Effect

In the context of the natural splitting, the standard relative dynamics can be expressed in terms of gravito-electromagnetic fields, which allow to formally introduce a gravito-magnetic Aharonov-Bohm effect. We showed elsewhere that this formal analogy can be used to derive the Sagnac effect in flat space-time as a gravito-magnetic Aharonov-Bohm effect. Here, we generalize those results to study the General Relativistic corrections to the Sagnac effect in some stationary and axially symmetric geometries, such as the space-time around a weakly gravitating and rotating source, Kerr space-time, G\"{odel} universe and Schwarzschild space-time.Comment: 14 pages, 1 EPS figure, LaTeX, accepted for publication in General Relativity and Gravitatio

Presencia de Pseudosuccinea columella naturalmente infestada con Fasciola hepatica en Santo Tomé (Corrientes, Argentina)

La fasciolosis es una enfermedad producida por Fasciola hepatica. Esta parasitosis produce grandes pérdidas económicas en el ganado bovino de la Provincia de Corrientes, Argentina, y también tiene carácter zoonótico. En el norte de la Provincia, Pseudosuccinea columella es el caracol involucrado en la transmisión de la enfermedad. El objetivo del presente trabajo fue ampliar los datos sobre la distribución de P. columella y su infestación natural por F. hepatica en la Provincia de Corrientes. Se recolectaron caracoles en un establecimiento ganadero del Departamento de Santo Tomé, al nordeste de la Provincia, que fueron identificados taxonómicamente, medidos y disecados para verificar la presencia de cercarias de F. hepatica. Los 115 caracoles recolectados fueron identificados como P. columella. La longitud de la conchilla varió entre 0,8–21,3 mm, con una media de 10,0 mm. La prevalencia de caracoles naturalmente infestados por F. hepatica fue de 8 (7,4%) sobre 108 examinados. La presencia de P. columella en distintos departamentos del norte y nordeste de la Provincia de Corrientes y las altas prevalencias de infestación natural por F. hepatica registradas, indicarían el asentamiento de un ciclo de transmisión enzoótica en esta zona

Allergen Sensitization and Asthma Outcomes among World Trade Center Rescue and Recovery Workers

A large number of World Trade Center (WTC) rescue and recovery workers are affected by asthma. While physical and mental health comorbidities have been associated with poor asthma control in this population, the potential role of allergen sensitization is unknown. This study examined the association of indoor sensitization and exposure as a risk factor for increased asthma morbidity in WTC workers. We used data from a prospective cohort of 331 WTC workers with asthma. Sensitization to indoor allergens was assessed by measurement of antigen-specific serum immunoglobulin E (IgE) levels. We used validated tools to evaluate the exposure to indoor allergens. Asthma morbidity outcomes included level of control (Asthma Control Questionnaire, ACQ), quality of life (Asthma Quality of Life Questionnaire, AQLQ) and acute resource utilization. The prevalence of sensitization to cat, dog, mouse, dust mite, cockroach, and mold allergens were 33%, 21%, 17%, 40%, 17%, and 17%, respectively. Unadjusted and regression analyses showed no significant relationship between sensitization and increased asthma morbidity (p \u3e 0.05 for all comparisons), except for sensitization to Aspergillus Fumigatus, cat and mouse epithelium, which were associated with decreased morbidity

The modified patient enablement instrument: a Portuguese cross-cultural adaptation, validity and reliability study

"Article number: 16087"Enabling patients with asthma to obtain the knowledge, confidence and skills they need in order to assume a major role in the management of their disease is cost effective. It should be an integral part of any plan for long-term control of asthma. The modified Patient Enablement Instrument (mPEI) is an easily administered questionnaire that was adapted in the United Kingdom to measure patient enablement in asthma, but its applicability in Portugal is not known. Validity and reliability of questionnaires should be tested before use in settings different from those of the original version. The purpose of this study was to test the applicability of the mPEI to Portuguese asthma patients after translation and cross-cultural adaptation, and to verify the structural validity, internal consistency and reproducibility of the instrument. The mPEI was translated to Portuguese and back translated to English. Its content validity was assessed by a debriefing interview with 10 asthma patients. The translated instrument was then administered to a random sample of 142 patients with persistent asthma. Structural validity and internal consistency were assessed. For reproducibility analysis, 86 patients completed the instrument again 7 days later. Item-scale correlations and exploratory factor analysis were used to assess structural validity. Cronbach's alpha was used to test internal consistency, and the intra-class correlation coefficient was used for the analysis of reproducibility. All items of the Portuguese version of the mPEI were found to be equivalent to the original English version. There were strong item-scale correlations that confirmed construct validity, with a one component structure and good internal consistency (Cronbach's alpha >0.8) as well as high test-retest reliability (ICC=0.85). The mPEI showed sound psychometric properties for the evaluation of enablement in patients with asthma making it a reliable instrument for use in research and clinical practice in Portugal. Further studies are needed to confirm its responsiveness.Financial support for this work was provided by FEDER funds through the Operational Programme Competitiveness Factors-COMPETE and National Funds through FCT-Foundation for Science and Technology under the project POCI-01-0145-FEDER-007038, and by the project NORTE-01-0145-FEDER-000013, supported by Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).info:eu-repo/semantics/publishedVersio