Lamb Wave Mode Selection for Increased Sensitivity ot Interfacial Weaknesses of Adhesive Bonds

Interface quality between layers in a layered structure is critical in fracture and fatigue analysis. A theoretical and quantitative solution to the problem from a NDE point of view would be desirable in both manufacturing and for in-service investigation of a variety of different structures. For example a great need exists to develop a reliable and efficient inspection program of adhesive bond delamination and interfacial weakness detection in aging aircraft noting that the bond degradation generally preceeds cracking in the aluminum skin, starting at the rivet holes

Over-communicate no more: Situated RL agents learn concise communication protocols

While it is known that communication facilitates cooperation in multi-agent settings, it is unclear how to design artificial agents that can learn to effectively and efficiently communicate with each other. Much research on communication emergence uses reinforcement learning (RL) and explores unsituated communication in one-step referential tasks -- the tasks are not temporally interactive and lack time pressures typically present in natural communication. In these settings, agents may successfully learn to communicate, but they do not learn to exchange information concisely -- they tend towards over-communication and an inefficient encoding. Here, we explore situated communication in a multi-step task, where the acting agent has to forgo an environmental action to communicate. Thus, we impose an opportunity cost on communication and mimic the real-world pressure of passing time. We compare communication emergence under this pressure against learning to communicate with a cost on articulation effort, implemented as a per-message penalty (fixed and progressively increasing). We find that while all tested pressures can disincentivise over-communication, situated communication does it most effectively and, unlike the cost on effort, does not negatively impact emergence. Implementing an opportunity cost on communication in a temporally extended environment is a step towards embodiment, and might be a pre-condition for incentivising efficient, human-like communication

Phenotypic Detection of Clonotypic B Cells in Multiple Myeloma by Specific Immunoglobulin Ligands Reveals their Rarity in Multiple Myeloma

In multiple myeloma, circulating “clonotypic” B cells, that express the immunoglobulin rearrangement of the malignant plasma cell clone, can be indirectly detected by PCR. Their role as potential “feeder” cells for the malignant plasma cell pool remains controversial. Here we established for the first time an approach that allows direct tracking of such clonotypic cells by labeling with patient-specific immunoglobulin ligands in 15 patients with myeloma. Fifty percent of patients showed evidence of clonotypic B cells in blood or bone marrow by PCR. Epitope-mimicking peptides from random libraries were selected on each patient's individual immunoglobulin and used as ligands to trace cells expressing the idiotypic immunoglobulin on their surface. We established a flow cytometry and immunofluorescence protocol to track clonotypic B cells and validated it in two independent monoclonal B cell systems. Using this method, we found clonotypic B cells in only one out of 15 myeloma patients. In view of the assay's validated sensitivity level of 10−3, this surprising data suggests that the abundance of such cells has been vastly overestimated in the past and that they apparently represent a very rare population in myeloma. Our novel tracing approach may open perspectives to isolate and analyze clonotypic B cells and determine their role in myeloma pathobiology

Ultrasonic Measurement of Elastic Constants for Composite Overlays

Unidirectional boron fiber-epoxy composites are used for crack repair and for reinforcement of highly stressed regions in aircraft components and structures [1]. Critical nondestructive evaluation problems related to such repair technology include the need to ensure the integrity of the bond between the composite reinforcement and the substrate, and to detect and measure the depth of a crack underneath the reinforcement. Among possible ultrasonic techniques, leaky interface waves have shown promise for the measurement of adhesive bond strength [2], and could also allow extension to second-layer cracks of crack depth measurement techniques such as Rayleigh wave spectral modulation [3,4]. However, it is first necessary to measure elastic constants, Cij, for the composite, as these constants are needed to determine whether leaky interlace waves occur for a particular composite/substrate combination. Note that it is insufficient to measure Cij for composite material nominally identical to that used in a specific repair application, as the existence or otherwise of interface waves can be altered by small variations in Cij

A Comparison of Different Methods for the Detection of a Weak Adhesive/Adherend Interface in Bonded Joints

There are three main classes of defect which occur in adhesive joints: complete disbonds, voids or porosity in the adhesive layer, poor cohesion (ie a weak adhesive layer) and poor adhesion (ie a weak interface between the adhesive layer and one or both adherends). The detection of disbonds, voids and porosity generally presents few problems and significant progress has been made towards the development of techniques for monitoring the cohesive properties of the adhesive layer [1]. However, there is no satisfactory method for the detection of a weak interface between the adhesive and the adherend(s) and this remains one of the major challenges in NDE. It is the interlayer which is affected by the common problem of slight contamination due to, for example, grease on the adherend surfaces prior to bonding. The adhesive/adherend interface is particularly important in aluminium-aluminium joints in which an inappropriate interface structure can cause greatly enhanced susceptibility to environmental attack [2]. Inspection of the interlayer is difficult because it is frequently only of the order of 1µm thick, compared with an adhesive layer thickness of the order of 100 µm

Challenges in the management of a patient with Cowden syndrome: case report and literature review

We would like to present a patient with a classical phenotype of a rare disorder - Cowden syndrome, its diagnostics and management challenges. A breast surgeon has to be aware of this rare condition when treating a patient with breast manifestations of Cowden syndrome and has to refer the patient to a clinical geneticist for further evaluation. Sequencing of the PTEN gene showed the Asp24Gly mutation. According to the latest literature data, the lifetime risk of breast cancer for Cowden syndrome patients is 81% and surgery is a justified option to reduce the risk of breast cancer. Bilateral risk-reducing mastectomy with immediate reconstruction was performed to eliminate further risk of breast cancer. 3 years after the risk-reducing breast surgery the patient is satisfied with the outcome. This is to our best knowledge the first reported Cowden syndrome case with follow-up data after risk-reducing measures have been taken

A Single Nucleotide Polymorphism in the RASGRF2 Gene Is Associated with Alcoholic Liver Cirrhosis in Men

Background Genetic polymorphisms in the RAS gene family are associated with different diseases, which may include alcohol-related disorders. Previous studies showed an association of the allelic variant rs26907 in RASGRF2 gene with higher alcohol intake. Additionally, the rs61764370 polymorphism in the KRAS gene is located in a binding site for the let-7 micro-RNA family, which is potentially involved in alcohol-induced inflammation. Therefore, this study was designed to explore the association between these two polymorphisms and susceptibility to alcoholism or alcoholic liver disease (ALD). Methods We enrolled 301 male alcoholic patients and 156 healthy male volunteers in this study. Polymorphisms were genotyped by using TaqMan® PCR assays for allelic discrimination. Allelic and genotypic frequencies were compared between the two groups. Logistic regression analysis was performed to analyze the inheritance model. Results The A allele of the RASGRF2 polymorphism (rs26907) was significantly more prevalent among alcoholic patients with cirrhosis (23.2%) compared to alcoholic patients without ALD (14.2%). This difference remained significant in the group of patients with alcohol dependence (28.8% vs. 14.3%) but not in those with alcohol abuse (15.1% vs. 14.4%). Multivariable logistic regression analysis showed that the A allele of this polymorphism (AA or GA genotype) was associated with alcoholic cirrhosis both in the total group of alcoholics (odds ratio [OR]: 2.33, 95% confidence interval [CI]: 1.32–4.11; P = 0.002) and in the group of patients with alcohol dependence (OR: 3.1, 95% CI: 1.50–6.20; P = 0.001). Allelic distributions of the KRAS polymorphism (rs61764370) did not differ between the groups. Conclusions To our knowledge, this genetic association study represents the first to show an association of the RASGRF2 G>A (rs26907) polymorphism with ALD in men, particularly in the subgroup of patients with AD. The findings suggest the potential relevance of the RAS gene family in alcoholism and ALD

Respostas fisiológicas da tilápia-do-nilo e variáveis limnológicas após decomposição de macrófitas utilizando herbicidas.

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Common genetic variants of fetal hemoglobin modify hematological phenotypes in MDS/MPN/Myeloma patients receiving cytotoxic drugs

Genetic studies identify common variants within the HBS1L-MYB intergenic region (HMIP), BCL11A, and Xmn1-HBG2 as associated with elevated fetal hemoglobin (HbF) levels and other clinically important human hematological traits. Recent studies suggest HbF is a predictor of outcome in MDS/AML patients receiving decitabine. We assessed effects of HbF genetic variants on hematological traits in myeloproliferative neoplasm (MPN), myelodysplastic syndrome (MDS) and myeloma on HbF-inducing therapy to determine potential for variants predicting treatment response. Seven common HbF variants at HMIP, BCL11A, Xmn1-HGB2 loci were genotyped in 89 patients with MPN on Hydroxyurea (HU), myeloma on Lenalidomide, and MDS on Azacytidine. HbF genetic association was seen with rs9494142 (HMIP) in MPN on HU (p = 0.04) and rs1427407 (BCL11A) in myeloma on Lenalidomide (p = 0.002). HMIP variants rs9494142 and rs6920211 influenced baseline platelets (p = 0.04) and hemoglobin treatment response (p = 0.02). rs1427407 (BCL11A) was significantly associated with increased platelets (p = 0.04) negating thrombocytopenic tendency of Lenalidomide. These HbF variants showed significantly discordant minor allele frequencies in MDS/MPN/myeloma compared to wider European population data. This small study findings together suggest the implication of these variants in treatment response and disease biology in MDS/MPN/myeloma warranting larger prospective genotype-phenotype association studies

Live lecture versus video podcast in undergraduate medical education: A randomised controlled trial

<p>Abstract</p> <p>Background</p> <p>Information technology is finding an increasing role in the training of medical students. We compared information recall and student experience and preference after live lectures and video podcasts in undergraduate medical education.</p> <p>Methods</p> <p>We performed a crossover randomised controlled trial. 100 students were randomised to live lecture or video podcast for one clinical topic. Live lectures were given by the same instructor as the narrator of the video podcasts. The video podcasts comprised Powerpoint™ slides narrated using the same script as the lecture. They were then switched to the other group for a second clinical topic. Knowledge was assessed using multiple choice questions and qualitative information was collected using a questionnaire.</p> <p>Results</p> <p>No significant difference was found on multiple choice questioning immediately after the session. The subjects enjoyed the convenience of the video podcast and the ability to stop, review and repeat it, but found it less engaging as a teaching method. They expressed a clear preference for the live lecture format.</p> <p>Conclusions</p> <p>We suggest that video podcasts are not ready to replace traditional teaching methods, but may have an important role in reinforcing learning and aiding revision.</p