Polyamine regulation of nitric oxide production in LPS-activated macrophages

© European Communities, 1999 Reproduction is authorised provided the source is acknowledgedPolyamines are physiological cellular constituents essential for cell growth and differentiation, and regulate a multitude of cellular functions (1-4). Nitric oxide (NO) is an effector molecule in both the cardiovascular and nervous systems (5,6). Intracellularly, NO and polyamines are derived from arginine, the latter via the rate-limiting enzyme ornithine decarboxylase (ODC; 7). This enzyme, like the inducible nitric oxide synthase (iNOS), is induced by proinflammatory cytokines and bacterial lipopolysaccharide (LPS), resulting in enhanced enzyme activity and increased polyamine biosynthesis (8,9). While the increase in polyamine synthesis would have important implications for cell growth and proliferation, it is not clear how this might affect iNOS pathway. Inhibition of polyamine biosynthesis impairs the phagocytic capacity of macrophages (10) and can block macrophage activation by tumour necrosis factor (11). Recently, exogenous polyamines have been shown to inhibit NO production in LPS-activated J774 cells (12) and by isolated neuronal NO synthase (13). However, these effects required relatively high concentrations of polyamines compared to those found in plasma and in intact cells (14), and appear to be due to aldehyde metabolites resulting from polyamine oxidation by the amine oxidase present in calf serum (15-17). In this study we have explored the effects of both endogenous and exogenous polyamines on the inducible L-arginine-NO pathway by examining whether inhibition of ornithine decarboxylase (ODC) and thus of polyamine biosynthesis (7), regulates NO production in lipopolysaccharide-activated J774 cells, a murine macrophage cell lineFinal Published versio

The provision of distance education within the HE sector - some areas for concern

This paper presents a summary of the findings of a recent survey of the way in which UK higher education institutions (HEIs) are offering distance education (DE) courses, the types of courses being offered, and their modes of delivery. From analysis of the findings of this survey, it is apparent that the emphasis of HEIs is very much on the exploitation of available teaching technology in the delivery of DE courses. However, teaching at a distance is quite different from face-toface teaching, and the evidence suggests that many HEIs fail to implement any meaningful academic staff training for the new role of DE tutor. The authors consider the difficulties this presents to academic staff who are required to move from face-to-face teaching to online facilitating. The paper concludes with an examination of the current provision of staff development and training within UK HEIs and suggests the type of academic staff training required if DE courses are to become truly core activities

Testing equality of variances in the analysis of repeated measurements

The problem of comparing the precisions of two instruments using repeated measurements can be cast as an extension of the Pitman-Morgan problem of testing equality of variances of a bivariate normal distribution. Hawkins (1981) decomposes the hypothesis of equal variances in this model into two subhypotheses for which simple tests exist. For the overall hypothesis he proposes to combine the tests of the subhypotheses using Fisher's method and empirically compares the component tests and their combination with the likelihood ratio test. In this paper an attempt is made to resolve some discrepancies and puzzling conclusions in Hawkins's study and to propose simple modifications.\ud \ud The new tests are compared to the tests discussed by Hawkins and to each other both in terms of the finite sample power (estimated by Monte Carlo simulation) and theoretically in terms of asymptotic relative efficiencies

Beat Cepheid Period Ratios from OPAL Opacities

The discovery of a large number of beat Cepheids in the Large Magellanic Cloud in the MACHO survey, provides an opportunity to compare the characteristics of such Cepheids over a range of metallicities. We produced a large grid of linear nonadiabatic pulsation models using the OPAL opacity tables and with compositions corresponding to those of the Milky Way, and the Large and Small Magellanic Clouds. Using the relationship between the period ratio and the main pulsation period, we are able to define a range of models which correspond to the observed beat Cepheids, and thereby constrain the physical characteristics of the LMC beat Cepheids. We are also able to make some predictions about the nature of the yet-to-be-discovered SMC beat Cepheids.Comment: 20 pages, 6 embedded Postscript figures, uses aasms4.sty. Accepted for publication in the 1997 September A

Rapid toxicity detection in water quality control utilizing automated multispecies biomonitoring for permanent space stations

The objective of this study was to evaluate proposed design characteristics and applications of automated biomonitoring devices for real-time toxicity detection in water quality control on-board permanent space stations. Simulated tests in downlinking transmissions of automated biomonitoring data to Earth-receiving stations were simulated using satellite data transmissions from remote Earth-based stations

Teaching varies with task complexity in wild chimpanzees

Understanding social influences on how apes acquire tool behaviors can help us model the evolution of culture and technology in humans. Humans scaffold novice tool skills with diverse strategies, including the transfer of tools between individuals. Chimpanzees transfer tools, and this behavior meets criteria for teaching. However, it is unclear how task complexity relates to this form of helping. Here, we find differences between 2 wild chimpanzee populations in rate, probability, and types of tool transfer during termite gathering. Chimpanzees showed greater helping in the population where termite gathering is a more complex tool task. In wild chimpanzees, as in humans, regular and active provisioning of learning opportunities may be essential to the cultural transmission of complex skills.Cumulative culture is a transformative force in human evolution, but the social underpinnings of this capacity are debated. Identifying social influences on how chimpanzees acquire tool tasks of differing complexity may help illuminate the evolutionary origins of technology in our own lineage. Humans routinely transfer tools to novices to scaffold their skill development. While tool transfers occur in wild chimpanzees and fulfill criteria for teaching, it is unknown whether this form of helping varies between populations and across tasks. Applying standardized methods, we compared tool transfers during termite gathering by chimpanzees in the Goualougo Triangle, Republic of Congo, and in Gombe, Tanzania. At Goualougo, chimpanzees use multiple, different tool types sequentially, choose specific raw materials, and perform modifications that improve tool efficiency, which could make it challenging for novices to manufacture suitable tools. Termite gathering at Gombe involves a single tool type, fishing probes, which can be manufactured from various materials. Multiple measures indicated population differences in tool-transfer behavior. The rate of transfers and probability of transfer upon request were significantly higher at Goualougo, while resistance to transfers was significantly higher at Gombe. Active transfers of tools in which possessors moved to facilitate possession change upon request occurred only at Goualougo, where they were the most common transfer type. At Gombe, tool requests were typically refused. We suggest that these population differences in tool-transfer behavior may relate to task complexity and that active helping plays an enhanced role in the cultural transmission of complex technology in wild apes

Applications of satellite technology to broadband ISDN networks

Two satellite architectures for delivering broadband integrated services digital network (B-ISDN) service are evaluated. The first is assumed integral to an existing terrestrial network, and provides complementary services such as interconnects to remote nodes as well as high-rate multicast and broadcast service. The interconnects are at a 155 Mbs rate and are shown as being met with a nonregenerative multibeam satellite having 10-1.5 degree spots. The second satellite architecture focuses on providing private B-ISDN networks as well as acting as a gateway to the public network. This is conceived as being provided by a regenerative multibeam satellite with on-board ATM (asynchronous transfer mode) processing payload. With up to 800 Mbs offered, higher satellite EIRP is required. This is accomplished with 12-0.4 degree hopping beams, covering a total of 110 dwell positions. It is estimated the space segment capital cost for architecture one would be about $190M whereas the second architecture would be about$250M. The net user cost is given for a variety of scenarios, but the cost for 155 Mbs services is shown to be about $15-22/minute for 25 percent system utilization

Orbit transfer rocket engine integrated control and health monitoring system technology readiness assessment

The objectives of this task were to: (1) estimate the technology readiness of an integrated control and health monitoring (ICHM) system for the Aerojet 7500 lbF Orbit Transfer Vehicle engine preliminary design assuming space based operations; and (2) estimate the remaining cost to advance this technology to a NASA defined 'readiness level 6' by 1996 wherein the technology has been demonstrated with a system validation model in a simulated environment. The work was accomplished through the conduct of four subtasks. In subtask 1 the minimally required functions for the control and monitoring system was specified. The elements required to perform these functions were specified in Subtask 2. In Subtask 3, the technology readiness level of each element was assessed. Finally, in Subtask 4, the development cost and schedule requirements were estimated for bringing each element to 'readiness level 6'