75 research outputs found

    Reconstruction of the mandible with an autogenous corticocancellous bone graft and fibrin glue: An animal experiment

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    This paper reports on an experimental animal study evaluating a method of mandibular reconstruction in dogs using autogenous corticocaneclous bone graft and fibrin glue. Eight animals underwent a resection at the mandibular body and primary reconstruction was carried out using osteosynthesis plates and screws. The defect was bridged with an autogenous particulate bone graft from the anterior iliac crest. To accelerate bone healing, fibrin glue (FG) was mixed with particulate bone graft. The hypothesis of this study was based on the presumption that bone healing, in segmental reconstruction of the dog mandible with a particulate cancellous bone graft mixed with FG, would be successful. All dogs had eventful healing. The histological results were less favorable with regard to bone remodeling than the results obtained in similar experiments with a particulate corticocancellous bone graft

    The influence of low-frequency magnetic field regions on the Saccharomyces cerevisiae respiration and growth

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    The influence of four low-frequency magnetic field (MF) ranges 10–300 Hz, 10–100 Hz, 10–50 Hz and 50–100 Hz in scanning regime (all frequencies from selected range were scanned during 100 s repetitively during 24 h) on baker's yeast cells Saccharomyces cerevisiae was examined by continuous measurements of cumulative O2 consumption and cumulative CO2 production over 24 h with Micro-Oxymax® respirometer. Besides respiration activity, measurements of cell growth and glucose uptake were performed as well. Statistical analysis indicated that, among all investigated low-frequency MF ranges, range from 10 Hz to 50 Hz had the greatest influence to yeast cell respiration and cell growth. More precisely, for this region, paired two sample one-tail t-test showed statistically significant differences in cumulative O2 consumption, cumulative CO2 production and S. cerevisiae cell number. Moreover samples exposed to MF range from 10 Hz to 50 Hz showed the same behavior in all five replicates: lower cumulative O2 consumption, higher cumulative CO2 production and higher cell number compared to control sample. This could be important from the application aspect, in industry (food, feed, brewery etc.) and biotechnology, because changes in cells metabolism are not caused by chemical treatment.Related to published version: [http://cherry.chem.bg.ac.rs/handle/123456789/3321

    Kinetics of the Bray-Liebhafsky oscillatory reaction perturbed by polymer supported cobalt catalyst

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    The Bray-Liebhafsky (BL) oscillatory reaction generated in the batch reactor at 62- 68 oC was perturbed by cobalt(II)-nitrate, supported on the macroreticular copolymer of poly-4-vinylpyridine with divinylbenzene (Co-PVPDVB). The kinetic data was analyzed of the complex pathways of the hydrogen peroxide decomposition in the examined BL reaction. The obtained results confirm that the kinetics of the BL reaction in the presence Co-PVPDVB comes partially from the Co-catalyst and partially from the macroreticular copolymer support.Oscilatorna reakcija Bray-Liebhafsky (BL) realizovana u zatvorenom reaktoru na temperaturi 62-68 oC je perturbovana sa kobalt(II)nitratom ugrađenim na makroretikularnom kopolimeru poli-4-vinilpiridina sa divinilbenzenom (Co- PVPDVB). Analizirani su kinetički podaci kompleksnih reakcionih puteva razlaganja vodonikperoksida u ispitivanoj BL reakciji. Dobijeni rezultati potvrđuju da je u prisustvu Co-PVPDVB kinetika BL reakcije delimično određena Co-katalizatorom, a delimično makromolekularnim kopolimernim nosačem katalizatora

    Changes in expression of GFAP, ApoE and APP mRNA and protein levels in the adult rat brain following cortical injury

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    The recovery period following cortical injury (CI) is characterized by a dynamic and highly complex interplay between beneficial and detrimental events. The aim of this study was to examine the expressions of Glial Fibrillary Acidic Protein (GFAP), Apolipoprotein E (ApoE) and Amyloid Precursor Protein (APP), all of which are involved in brain plasticity and neurodegeneration. Our results reveal that CI strongly influenced GFAP, ApoE and APP mRNA expression, as well as GFAP and ApoE protein expression. Considering the pivotal role of these proteins in the brain, the obtained results point to their potential contribution in neurodegeneration and consequent Alzheimer's disease development.Projekat ministarstva br. 17305
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