Identification of candidate genes affecting Delta9-tetrahydrocannabinol biosynthesis in Cannabis sativa.

RNA isolated from the glands of a Delta(9)-tetrahydrocannabinolic acid (THCA)-producing strain of Cannabis sativa was used to generate a cDNA library containing over 100 000 expressed sequence tags (ESTs). Sequencing of over 2000 clones from the library resulted in the identification of over 1000 unigenes. Candidate genes for almost every step in the biochemical pathways leading from primary metabolites to THCA were identified. Quantitative PCR analysis suggested that many of the pathway genes are preferentially expressed in the glands. Hexanoyl-CoA, one of the metabolites required for THCA synthesis, could be made via either de novo fatty acids synthesis or via the breakdown of existing lipids. qPCR analysis supported the de novo pathway. Many of the ESTs encode transcription factors and two putative MYB genes were identified that were preferentially expressed in glands. Given the similarity of the Cannabis MYB genes to those in other species with known functions, these Cannabis MYBs may play roles in regulating gland development and THCA synthesis. Three candidates for the polyketide synthase (PKS) gene responsible for the first committed step in the pathway to THCA were characterized in more detail. One of these was identical to a previously reported chalcone synthase (CHS) and was found to have CHS activity. All three could use malonyl-CoA and hexanoyl-CoA as substrates, including the CHS, but reaction conditions were not identified that allowed for the production of olivetolic acid (the proposed product of the PKS activity needed for THCA synthesis). One of the PKS candidates was highly and specifically expressed in glands (relative to whole leaves) and, on the basis of these expression data, it is proposed to be the most likely PKS responsible for olivetolic acid synthesis in Cannabis glands

The Role of a Hot Gas Environment on the Evolution of Galaxies

Most spiral galaxies are found in galaxy groups with low velocity dispersions; most E/S0 galaxies are found in galaxy groups with relatively high velocity dispersions. The mass of the hot gas we can observe in the E/S0 groups via their thermal X-ray emission is, on average, as much as the baryonic mass of the galaxies in these groups. By comparison, galaxy clusters have as much or more hot gas than stellar mass. Hot gas in S-rich groups, however, is of low enough temperature for its X-ray emission to suffer heavy absorption due to Galactic HI and related observational effects, and hence is hard to detect. We postulate that such lower temperature hot gas does exist in low velocity dispersion, S-rich groups, and explore the consequences of this assumption. For a wide range of metallicity and density, hot gas in S-rich groups can cool in far less than a Hubble time. If such gas exists and can cool, especially when interacting with HI in existing galaxies, then it can help link together a number of disparate observations, both Galactic and extragalactic, that are otherwise difficult to understand.Comment: 16 pages with one figure. ApJ Letters, in pres

Identification of candidate genes affecting Δ9-tetrahydrocannabinol biosynthesis in Cannabis sativa

RNA isolated from the glands of a Δ9-tetrahydrocannabinolic acid (THCA)-producing strain of Cannabis sativa was used to generate a cDNA library containing over 100 000 expressed sequence tags (ESTs). Sequencing of over 2000 clones from the library resulted in the identification of over 1000 unigenes. Candidate genes for almost every step in the biochemical pathways leading from primary metabolites to THCA were identified. Quantitative PCR analysis suggested that many of the pathway genes are preferentially expressed in the glands. Hexanoyl-CoA, one of the metabolites required for THCA synthesis, could be made via either de novo fatty acids synthesis or via the breakdown of existing lipids. qPCR analysis supported the de novo pathway. Many of the ESTs encode transcription factors and two putative MYB genes were identified that were preferentially expressed in glands. Given the similarity of the Cannabis MYB genes to those in other species with known functions, these Cannabis MYBs may play roles in regulating gland development and THCA synthesis. Three candidates for the polyketide synthase (PKS) gene responsible for the first committed step in the pathway to THCA were characterized in more detail. One of these was identical to a previously reported chalcone synthase (CHS) and was found to have CHS activity. All three could use malonyl-CoA and hexanoyl-CoA as substrates, including the CHS, but reaction conditions were not identified that allowed for the production of olivetolic acid (the proposed product of the PKS activity needed for THCA synthesis). One of the PKS candidates was highly and specifically expressed in glands (relative to whole leaves) and, on the basis of these expression data, it is proposed to be the most likely PKS responsible for olivetolic acid synthesis in Cannabis glands

Transcription profiling reveals potential mechanisms of dysbiosis in the oral microbiome of rhesus macaques with chronic untreated SIV infection.

A majority of individuals infected with human immunodeficiency virus (HIV) have inadequate access to antiretroviral therapy and ultimately develop debilitating oral infections that often correlate with disease progression. Due to the impracticalities of conducting host-microbe systems-based studies in HIV infected patients, we have evaluated the potential of simian immunodeficiency virus (SIV) infected rhesus macaques to serve as a non-human primate model for oral manifestations of HIV disease. We present the first description of the rhesus macaque oral microbiota and show that a mixture of human commensal bacteria and "macaque versions" of human commensals colonize the tongue dorsum and dental plaque. Our findings indicate that SIV infection results in chronic activation of antiviral and inflammatory responses in the tongue mucosa that may collectively lead to repression of epithelial development and impact the microbiome. In addition, we show that dysbiosis of the lingual microbiome in SIV infection is characterized by outgrowth of Gemella morbillorum that may result from impaired macrophage function. Finally, we provide evidence that the increased capacity of opportunistic pathogens (e.g. E. coli) to colonize the microbiome is associated with reduced production of antimicrobial peptides

Extracting the late-time kinetic Sunyaev-Zel'dovich effect

We propose a novel technique to separate the late-time, post-reionization component of the kinetic Sunyaev Zeldovich (kSZ) effect from the contribution to it from a (poorly understood and probably patchy) reionization history. The kSZ effect is one of the most promising probe of the missing baryons in the Universe. We study the possibility of reconstructing it in three dimensions (3D), using future spectroscopic surveys such as the Euclid survey. By reconstructing a 3D template from galaxy density and peculiar velocity fields from spectroscopic surveys we cross-correlate the estimator against CMB maps. The resulting cross-correlation can help us to map out the kSZ contribution to CMB in 3D as a function of redshift thereby extending previous results which use tomographic reconstruction. This allows the separation of the late-time effect from the contribution owing to reionization. By construction, it avoids contamination from foregrounds, primary CMB, tSZ effect as well as from star-forming galaxies. Due to a high number density of galaxies the signal-to-noise ratio (S/N) for such cross-correlational studies is higher, compared to the studies involving CMB power-spectrum analysis. Using a spherical Bessel–Fourier (sFB) transform we introduce a pair of 3D power spectra: C⊥ℓ(k) and C⊥ℓ(k) that can be used for this purpose. We find that in a future spectroscopic survey with near all-sky coverage and a survey depth of z ≈ 1, reconstruction of C⊥ℓ(k) can be achieved in a few radial wave bands k ≈ (0.01–0.5 h−1 Mpc) with a S/N ratio of up to O(10) for angular harmonics in the range = (200–2000)

KSU Trombone Choir

Kennesaw State University School of Music presents Trombone Choir.https://digitalcommons.kennesaw.edu/musicprograms/1340/thumbnail.jp

Detection of Extended He II Reionization in the Temperature Evolution of the Intergalactic Medium

We present new measurements of the temperature of the intergalactic medium (IGM) derived from the Lyman-alpha forest over 2.0 < z < 4.8. The small-scale structure in the forest of 61 high-resolution QSO spectra is quantified using a new statistic, the curvature, and the conversion to temperature calibrated using a suite of hydrodynamic simulations. At each redshift we focus on obtaining the temperature at an optimal overdensity probed by the Lyman-alpha forest, T(Delta), where the temperature is nearly a one-to-one function of the curvature regardless of the slope of the temperature-density relation. The median 2-sigma statistical uncertainty in these measurements is 8 per cent, though there may be comparable systematic errors due to the unknown amount of Jeans smoothing in the IGM. We use our T(Delta) results to infer the temperature at the mean density, T0. Even for a maximally steep temperature-density relation, T0 must increase from ~8000 K at z ~ 4.4 to >~12000 K at z ~ 2.8. This increase is not consistent with the monotonic decline in T0 expected in the absence of He II reionization. We therefore interpret the observed rise in temperature as evidence of He II reionization beginning at z >~ 4.4. The evolution of T0 is consistent with an end to He II reionization at z ~ 3, as suggested by opacity measurements of the He II Lyman-alpha forest, although the redshift at which T0 peaks will depend somewhat on the evolution of the temperature-density relation. These new temperature measurements suggest that the heat input due to the reionization of He II dominates the thermal balance of the IGM over an extended period with Delta_z >~ 1.Comment: 19 pages, 15 figures, accepted to MNRA