Meteorological and operational aspects of 46 clear air turbulent sampling missions with an instrumented B-57B aircraft. Volume 2, appendix C: Turbulence missions

The results of 46 clear air turbulence (CAT) probing missions conducted with an extensively instrumented B-57B aircraft are summarized from a meteorological viewpoint in a two-volume technical memorandum. The missions were part of the NASA Langley Research Center's MAT (Measurement of Atmospheric Turbulence) program, which was conducted between March 1974, and September 1975, at altitudes ranging up to 15 km. Turbulence samples were obtained under diverse conditions including mountain waves, jet streams, upper level fronts and troughs, and low altitude mechanical and thermal turbulence. CAT was encountered on 20 flights comprising 77 data runs. In all, approximately 4335 km were flown in light turbulence, 1415 km in moderate turbulence, and 255 km in severe turbulence during the program

Performance of a frost hollow as a hemispherical thermal radiometer

Radiant sky hemispheric temperature, snow-surface temperature, and thermal profiles within the snowpack were measured at night in a frost hollow in southeastern Michigan, U.S.A. Snow-surface temperatures remained 3° to 5°C colder than air temperatures at 3 m above the snow surface and 6° to 7°C colder than air temperatures at 18 m, the height of the hollow's rim above its floor. Due to suppression of turbulent heat transfer, the energy balance at the surface was dominated by net longwave radiation; energy involved in sensible heat transfer through the snow was equal to only about 10% of the incoming longwave radiation. Incoming longwave radiation can be expressed as a linear function of surface temperature by means of a regression equation, which yields a coefficient of determination of 0.75. Die Strahlungstemperatur der Himmelshemisphäre, die Schneeoberflächentemperatur und thermische Profile in der Schneedecke wurden in einer klaren Nacht in einer Frostmulde im Südosten von Michigan, U.S.A., gemessen. Die Schneeoberflächentemperatur blieb 3 bis 5°C kälter als die Lufttemperatur in 3 m über der Schneeoberfläche und um 6 bis 7°C kälter als die Lufttemperatur in 18 m Höhe, das ist die Höhe des oberen Randes der Mulde über ihrem Boden. Bei Bestimmung der turbulenten Wärmeübertragung war der Energiehaushalt an der Oberfläche von der langwelligen Strahlungsbilanz beherrscht. Die mit der Transport fühlbarer Wärme durch den Schnee verbundene Energie betrug nur ungefähr 10% der langwelligen Einstrahlung. Die langwellige Einstrahlung kann durch eine lineare Funktion der Oberflächentemperatur mittels einer Regressionsgleichung ausgedrückt werden, die einen Regressionskoeffizienten von 0,75 ergibt.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/41662/1/703_2005_Article_BF02273978.pd

High-volume hybridoma sequencing on the NeuroMabSeq platform enables efficient generation of recombinant monoclonal antibodies and scFvs for neuroscience research

Abstract The Neuroscience Monoclonal Antibody Sequencing Initiative (NeuroMabSeq) is a concerted effort to determine and make publicly available hybridoma-derived sequences of monoclonal antibodies (mAbs) valuable to neuroscience research. Over 30 years of research and development efforts including those at the UC Davis/NIH NeuroMab Facility have resulted in the generation of a large collection of mouse mAbs validated for neuroscience research. To enhance dissemination and increase the utility of this valuable resource, we applied a high-throughput DNA sequencing approach to determine immunoglobulin heavy and light chain variable domain sequences from source hybridoma cells. The resultant set of sequences was made publicly available as a searchable DNA sequence database (neuromabseq.ucdavis.edu) for sharing, analysis and use in downstream applications. We enhanced the utility, transparency, and reproducibility of the existing mAb collection by using these sequences to develop recombinant mAbs. This enabled their subsequent engineering into alternate forms with distinct utility, including alternate modes of detection in multiplexed labeling, and as miniaturized single chain variable fragments or scFvs. The NeuroMabSeq website and database and the corresponding recombinant antibody collection together serve as a public DNA sequence repository of mouse mAb heavy and light chain variable domain sequences and as an open resource for enhancing dissemination and utility of this valuable collection of validated mAbs

Constructing Regularity Feature Trees for Solid Models

Approximate geometric models, e.g. as created by reverse engineering, describe the approximate shape of an object, but do not record the underlying design intent. Automatically inferring geometric aspects of the design intent, represented by feature trees and geometric constraints, enhances the utility of such models for downstream tasks. One approach to design intent detection in such models is to decompose them into regularity features. Geometric regularities such as symmetries may then be sought in each regularity feature, and subsequently be combined into a global, consistent description of the model’s geometric design intent. This paper describes a systematic approach for finding such regularity features based on recovering broken symmetries in the model. The output is a tree of regularity features for subsequent use in regularity detection and selection. Experimental results are given to demonstrate the operation and efficiency of the algorithm