The probiotic potential of lactobacilli isolated from Nile tilapia (Oreochromis niloticus)’s intestine

The potential probiotic acid lactic bacteria isolated from Nile tilapia (Oreochromis niloticus)fs intestine was tested for fish farming. In our collection, 10 Lactobacillus strains were targeted to confront a series of antibiotics in order to draw their resistance profile, and to test their degree of inhibitory to four pathogenic bacteria, Staphylococcus aureus, Escherichia coli, Streptococcus sp. and Pseudomonas sp. The power of acidification and tolerance was tested. Overall, our results show that strains BLT31 and BLT21 are fully susceptible and resistance to the tested antibiotics. Strains BLT3, BLT20, BLT21 and BLT23 have a good antagonistic effect against pathogenic bacteria that cause the highest damage in aquaculture. For acid lactic production, strains BLT3, BLT26, BLT27, BLT28, and BLT31 are considered fast since Ģ pH . 4U in less than three hours. As for the resistance to pH and bile salts, two strains BLT3 and BLT31 showed significant power which gives them acceptable probiotic potential.Key words: Probiotics, antibiotics, aquaculture, the Nile Tilapia, inhibitory activities, lactobacilli

Orientation Invariant ECG-Based Stethoscope Tracking for Heart Auscultation Training on Augmented Standardized Patients

Auscultation, the act of listening to the heart and lung sounds, can reveal substantial information about patients’ health and other cardiac-related problems; therefore, competent training can be a key for accurate and reliable diagnosis. Standardized patients (SPs), who are healthy individuals trained to portray real patients, have been extensively used for such training and other medical teaching techniques; however, the range of symptoms and conditions they can simulate remains limited since they are only patient actors. In this work, we describe a novel tracking method for placing virtual symptoms in correct auscultation areas based on recorded ECG signals with various stethoscope diaphragm orientations; this augmented reality simulation would extend the capabilities of SPs and allow medical trainees to hear abnormal heart and lung sounds in a normal SP. ECG signals recorded from two different SPs over a wide range of stethoscope diaphragm orientations were processed and analyzed to accurately distinguish four different heart auscultation areas, aortic, mitral, pulmonic and tricuspid, for any stethoscope’s orientation. After processing the signals and extracting relevant features, different classifiers were applied for assessment of the proposed method; 95.1% and 87.1% accuracy were obtained for SP1 and SP2, respectively. The proposed system provides an efficient, non-invasive, and cost efficient method for training medical practitioners on heart auscultation

Engineering Collaborations in Medical Modeling and Simulation

Fifty years ago computer science was just beginning to see common acceptance as a growing discipline and very few universities had a computer science department although other departments were utilizing computers and software to enhance their methodologies. We believe modeling and simulation (M&S) is on a similar path. Many other disciplines utilize M&S to enhance their methodologies but we also believe that M&S fundamentals can be essential in making better decisions by utilizing the appropriate model for the problem at hand, expanding the solution space through simulation, and understanding it through visualization and proper analyses. After our students learn these fundamentals, we offer the opportunity to apply them to varied application areas. One such application area is medical M&S, which is a broad area involving anatomical modeling, planning and training simulations, image-guided procedures and more. In this paper, we share several research projects involving M&S and the collaborations that make them possible

Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana

Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies is a risk factor for acquiring BU, the epidemiology and mode of M. ulcerans transmission is poorly understood. Here we have used high-throughput DNA sequencing and comparisons of the genomes of seven M. ulcerans isolates that appeared monomorphic by existing typing methods. We identified a limited number of single nucleotide polymorphisms (SNPs) and developed a real-time PCR SNP typing method based on these differences. We then investigated clinical isolates of M. ulcerans on which we had detailed information concerning patient location and time of diagnosis. Within the Densu river basin of Ghana we observed dominance of one clonal complex and local clustering of some of the variants belonging to this complex. These results reveal focal transmission and demonstrate, that micro-epidemiological analyses by SNP typing has great potential to help us understand how M. ulcerans is transmitted

Distribution of Mycobacterium ulcerans in Buruli Ulcer Endemic and Non-Endemic Aquatic Sites in Ghana

Mycobacterium ulcerans, the causative agent of Buruli ulcer, is an emerging environmental bacterium in Australia and West Africa. The primary risk factor associated with Buruli ulcer is proximity to slow moving water. Environmental constraints for disease are shown by the absence of infection in arid regions of infected countries. A particularly mysterious aspect of Buruli ulcer is the fact that endemic and non-endemic villages may be only a few kilometers apart within the same watershed. Recent studies suggest that aquatic invertebrate species may serve as reservoirs for M. ulcerans, although transmission pathways remain unknown. Systematic studies of the distribution of M. ulcerans in the environment using standard ecological methods have not been reported. Here we present results from the first study based on random sampling of endemic and non-endemic sites. In this study PCR-based methods, along with biofilm collections, have been used to map the presence of M. ulcerans within 26 aquatic sites in Ghana. Results suggest that M. ulcerans is present in both endemic and non-endemic sites and that variable number tandem repeat (VNTR) profiling can be used to follow chains of transmission from the environment to humans. Our results suggesting that the distribution of M. ulcerans is far broader than the distribution of human disease is characteristic of environmental pathogens. These findings imply that focal demography, along with patterns of human water contact, may play a major role in transmission of Buruli ulcer

Genomic Diversity and Evolution of Mycobacterium ulcerans Revealed by Next-Generation Sequencing

Mycobacterium ulcerans is the causative agent of Buruli ulcer, the third most common mycobacterial disease after tuberculosis and leprosy. It is an emerging infectious disease that afflicts mainly children and youths in West Africa. Little is known about the evolution and transmission mode of M. ulcerans, partially due to the lack of known genetic polymorphisms among isolates, limiting the application of genetic epidemiology. To systematically profile single nucleotide polymorphisms (SNPs), we sequenced the genomes of three M. ulcerans strains using 454 and Solexa technologies. Comparison with the reference genome of the Ghanaian classical lineage isolate Agy99 revealed 26,564 SNPs in a Japanese strain representing the ancestral lineage. Only 173 SNPs were found when comparing Agy99 with two other Ghanaian isolates, which belong to the two other types previously distinguished in Ghana by variable number tandem repeat typing. We further analyzed a collection of Ghanaian strains using the SNPs discovered. With 68 SNP loci, we were able to differentiate 54 strains into 13 distinct SNP haplotypes. The average SNP nucleotide diversity was low (average 0.06–0.09 across 68 SNP loci), and 96% of the SNP locus pairs were in complete linkage disequilibrium. We estimated that the divergence of the M. ulcerans Ghanaian clade from the Japanese strain occurred 394 to 529 thousand years ago. The Ghanaian subtypes diverged about 1000 to 3000 years ago, or even much more recently, because we found evidence that they evolved significantly faster than average. Our results offer significant insight into the evolution of M. ulcerans and provide a comprehensive report on genetic diversity within a highly clonal M. ulcerans population from a Buruli ulcer endemic region, which can facilitate further epidemiological studies of this pathogen through the development of high-resolution tools