Comparison of nucleic acid extraction platforms for detection of select biothreat agents for use in clinical resource limited settings

High-quality nucleic acids are critical for optimal PCR-based diagnostics and pathogen detection. Rapid sample processing time is important for the earliest administration of therapeutic and containment measures, especially in the case of biothreat agents. In this context, we compared the Fujifilm QuickGene-Mini80 to Qiagen\u27s QIAamp Mini Purification kits for extraction of DNA and RNA for potential use in austere settings. Qiagen (QIAamp) column-based extraction is the currently recommended purification platform by United States Army Medical Research Institute for Infectious Diseases for both DNA and RNA extraction. However, this sample processing system requires dedicated laboratory equipment including a centrifuge. In this study, we investigated the QuickGene-Mini80, which does not require centrifugation, as a suitable platform for nucleic acid extraction for use in resource-limited locations. Quality of the sample extraction was evaluated using pathogen-specific, real-time PCR assays for nucleic acids extracted from viable and γ-irradiated Bacillus anthracis, Yersinia pestis, vaccinia virus, Venezuelan equine encephalitis virus, or B. anthracis spores in buffer or human whole blood. QuickGene-Mini80 and QIAamp performed similarly for DNA extraction regardless of organism viability. It was noteworthy that γ-irradiation did not have a significant impact on real-time PCR for organism detection. Comparison with QIAamp showed a less than adequate performance of the Fujifilm instrument for RNA extraction. However, QuickGene-Mini80 remains a viable alternative to QIAamp for DNA extraction for use in remote settings due to extraction quality, time efficiency, reduced instrument requirements, and ease of use

Magnetic properties of soft magnetic cobalt-based alloy heat treated in presence of indium vapors

The effect of the indium presence on the magnetic properties of soft magnetic cobalt-based alloy during heat treatment (HT) was studied. It was shown that changes in magnetic characteristics were largely due to the impact mechanism of HT. The presence of indium vapors during HT increased the maximum magnetic permeability and changed magneto-optical hysteresis loops

Volitional self-regulation and emotional burnout of professional sambo-athletes who suffered from sports injuries

Objective: to study the level of volitional self-regulation and emotional burnout in male sambo-athletes who underwent sports injuries of various degrees of severity. Materials and methods: 60 male sambo-athletes were examined. They were divided into 3 groups of 20 people in each group based on types of sports injuries. Results: significantly (p=0.001) lower level of volitional self-regulation was found in the third group who underwent more injuries (total scale: Q1=6,0; Me=8,0; Q3=10,0; «perseverance»: Q1=5,0; Me=5,5; Q3=8,0; «self-control»: Q1=4,0; Me=4,5; Q3=5,0). More pronounced emotional burnout occured in athletes who had more injuries (Q1=64,5; Me=69,0; Q3=71,0; p=0,001). Inadequate emotional response and emotional and moral disorientation was leveled down with aggravation of the severity of injuries (Q1=25,0; Me=44,0; Q3=61,0; p=0,039). Adaptation systems strain is manifested by a marked emotional exhaustion and decrease in volitional self-regulation with an increase in the number of injuries. Conclusions: if incidence and severity of injuries increases in sambo-athletes the level of volitional self-regulation reduces and emotional burnout occurs, which is characterized by the stress of the system of psychological adaptation

DYNAMICS OF MORBIDITY OF POPULATION IN IRKUTSK BETWEEN DURING SOCIO-ECONOMIC REFORMS

This article presents an analysis of disease trends in selected age groups of the population of Irkutsk for the period of 1992-2009 and it is found that most of these trends are dependent on socio-economic factors. Built polynomial regression models revealed significant increase in morbidity of mental disorders in children, diseases of the nervous system and. the digestive system against opposing change prevalence of adolescent and. adult population for the analyzed period

SOCIO-DEMOGRAPHIC AND EPIDEMIOLOGICAL ASPECTS OF CHILDREN AND ADOLESCENTS HEALTH OF THE SMALLER ETHNIC GROUPS LIVING IN SIBERIA (BY EXAMPLE OF TOFALARS PEOPLE)

This article is devoted to analysis of pathologic affect of child's and adolescent's population, living on the Tofalariya territory, as well as socio-economic factors influencing the health of the native population in the study area according to a sociological study. In a study conducted a thorough medical examination of children living in Tofalaria. And also a sample case study of families was conducted. The article examines a set of negative socio-economic factors, typical to the territory of Tofalars habitation (one parent family, factors of poor housing, low family income, low levels of parental education, nutritional imbalances). We can identify the low availability of health care for this population group in combination with their low medical activity. The self-assessment of health among the population of Tofalariya is overstated, probably due to the significantly low level of information awareness about the prevention of disease and methods of solution these problems

Structure and magnetoelectric coupling of LiNi1-xCoxPO4 multiferroics

This work was supported by MES of RF (contract No. 3.6121.2017/8.9), Act 211 Government of RF (contract No. 02.A03.21.0006), and supported in part by FASO of Russia (theme “Flux” No. AAA-A18-118020190112-8). The equipment of the Ural Center for Shared Use “Modern nanotechnology” SNSM UrFU was used

Structure and magnetic properties of LiNi1-xCoxPO4 magnetoelectrics with x = (0, 0.1, and 0.2)

We present the magnetic properties of LiNi1-xCoxPO4 magnetoelectrics, with x = (0-0.2), and their analysis of concentration dependences. Samples have been synthesized by a glycerol-nitrate method. To refine crystal structure X-ray diffraction measurements were carried out. Magnetic measurements were performed at the external magnetic field of 500 Oe over the temperature range (2-300) K. The neutron powder diffraction patterns of LiNi0.9Co0.1PO4 were recorded over temperature interval from 4.4 K up to 25 K. The partial doping in the LiNi1-xCoxPO4 magnetoelectrics the Ni ions for Co ions leads to a narrowing of the temperature interval where the incommensurate phase is established. © Published under licence by IOP Publishing Ltd.3.6121.2017/8.902.The work was supported by MES of RF (contract No. 3.6121.2017/8.9), and by Act 211 Government of RF (contract No. 02.A03.21.0006)

Myotonia dystrophica of Rossolimo-Steinert-Kurshman (case history)

It is presented clinical examination of the patient with myotoniadystrophica of Rossolimo-Steinert-Kurshman. It is showed the variety of clinical symptoms (neurological and somatic) and importance of their value and recording by not only neurologists but cardiologists and endocrinologists.Приведено клиническое наблюдение за больным с дистрофическоймиотонией Россолимо-Штейнерта-Куршмана. Показаны многообразие клинической симптоматики (неврологической и соматической) и важность ее знания и учета не только неврологами, но и кардиологами, эндокринологами

Антиагрегантная и антикоагулянтная активность 7-О-гентиобиозида формононетина в условиях in vitro и in vivo

Aim. In experiments in vitro and in vivo it was investigated the effect of isoflavone 7-O-gentiobiozide formononetin (GBF) isolated from the roots of the plant Maackia аmurensis (Maackia amurensis Rupr. et Maxim.), on the processes of vascular-platelet and coagulation hemostasis.Materials and methods. In experiments using blood of healthy human plasma in concentrations of GBF 1,0–50,0 mM promoted dose-dependently ADP-induced weakening of platelet aggregation. Since the concentration of 10,0 mM GBF induced hypocoagulative changes in blood plasma, comparable with the effect of 0,2–0,5 IU/ml heparin. Revealed hypocoagulative effect was confirmed in the application thromboelastometry, showing pronounced hypocoagulation and a significant reduction in fibrin formation dynamics.Results. In chronic GBF oral administration to rats at a dose of 25 mg/kg was fixed almost a 10-fold reduction in ADP-induced platelet aggregation, with increased content of these cells in the peripheral blood. Furthermore, in these conditions, there was a pronounced effect of GBF’s hypocoagulation which was implemented in inhibiting reactions inner and outer tracks of blood clotting, reducing the rate of formation of fibrin and its mechanical density.Conclusion.Thus, in experiments in vitro and in vivo for the first time revealed the ability of isoflavone 7-O-gentiobiozideformononetin extracted from the roots Maackia amurensis, inhibit the processes of vascularplatelet and coagulation hemostasis. This fact is of great practical importance, because it opens the prospective of the development of a new drug that can reduce the risk of thrombosis in various cardiovascular diseases.Цель. В экспериментах in vitro и in vivo исследовать влияние изофлавоноида 7-О-гентиобиозида формононетина (ГБФ), выделенного из корней растения маакия амурская (Maackia amurensis Rupr. et Maxim.), на показатели сосудисто-тромбоцитарного и коагуляционного гемостаза.Материал и методы. В экспериментах in vitro использовалась плазма крови 19 практически здоровых добровольцев обоего пола в возрасте 23–34 года, не принимавших каких-либо лекарственных препаратов на протяжении как минимум 2 нед до забора крови. Изучение ГБФ проводилось с применением обедненной или обогащенной тромбоцитами плазмы, полученной в соответствии с имеющимися рекомендациями. В контрольных исследованиях применялась та же плазма, но с добавлением растворителя в конечных концентрациях 1,25– 2,5%. В качестве объекта сравнения в ходе изучения влияния ГБФ на коагуляционный гемостаз использовали гепаринизированную плазму крови человека с конечной концентрацией нефракционированного гепарина 0,2–0,5 МЕ/мл. Исследования in vivo выполнены на аутбредных крысах сток Wistar обоего пола массой 220–270 г. На протяжении всего периода наблюдения животные находились в условиях свободного доступа к воде и пище при нормальном чередовании светлого и темного времени суток. Для изучения влияния длительного введения ГБФ на показатели сосудисто-тромбоцитарного и коагуляционного гемостаза животные были разделены на четыре группы по 10–12 крыс в каждой. Первой и второй группам крыс на протяжении 10 сут перорально вводили ГБФ в виде крахмальной взвеси в дозе 25 мг/кг массы тела. Полученные результаты сравнивали с соответствующими показателями контрольных животных (третья и четвертая группы), которые в течение такого же периода времени получали эквиобъемные количества крахмальной слизи.Результаты. В опытах с использованием плазмы крови здоровых людей ГБФ в концентрациях 1,0–50,0 мМ способствовал дозозависимому ослаблению индуцируемой аденозиндифосфатом (АДФ) агрегации тромбоцитов. В концентрации 50,0 мМ ГБФ вызывал гипокоагуляционные сдвиги в плазме крови, сопоставимые с действием 0,2–0,5 МЕ/мл гепарина. Выявленный гипокоагуляционный эффект подтвердился при применении тромбоэластометрии, демонстрируя выраженную гипокоагуляцию и существенное снижение динамики фибринообразования. При хроническом энтеральном введении ГБФ крысам в дозе 25 мг/кг было зафиксировано почти 10-кратное снижение АДФ-индуцированной агрегации тромбоцитов на фоне увеличения содержания этих клеток в периферической крови. Кроме того, в этих условиях наблюдался выраженный гипоко-агуляционный эффект ГБФ, который реализовывался в торможении реакций внутреннего и внешнего путей свертываемости крови, снижении скорости образования фибрина и его механической плотности. Заключение. В экспериментах in vitro и in vivo впервые выявлена способность изофлавоноида 7-О-гентиобиозида формононетина, выделенного из корней маакии амурской, ингибировать показатели сосудисто-тромбоцитарного и коагуляционного гемостаза. Этот факт имеет важное практическое значение, поскольку открывает перспективу создания нового лекарственного средства, способного уменьшить вероятность возникновения тромбозов при различных сердечно-сосудистых заболеваниях