815 research outputs found
Comparative analysis of Bacillus weihenstephanensis KBAB4 spores obtained at different temperatures
The impact of Bacillus weihenstephanensis KBAB4 sporulation temperature history was assessed on spore heat resistance, germination and outgrowth capacity at a temperature range from 7 to 30 °C. Sporulation rate and efficiency decreased at low temperature, as cells sporulated at 12, 20 and 30 °C with approximately 99% efficiency, whereas at 7 °C and 10 °C, a maximum 15% of sporulation was reached. Spores formed at 30 °C showed the highest wet heat resistance at 95 °C, with spores formed at 7 and 10 °C displaying only survival of 15 min exposure at 70 °C, indicating their low level heat resistance. RT-PCR analysis revealed expression of sporulation sigma factor sigG, and germinant receptor operons gerI, gerK, gerL, gerR, gerS, and (plasmid-located) gerS2 to be activated in all sporulation conditions tested. Subsequent germination assays revealed a combination of inosine and L-Alanine to be very efficient, triggering over 99% of the spores to germinate, with spores obtained at 30 °C showing the highest germination rates (99%). Notably, spores obtained at 12, 20 and 30 °C, germinated at all tested temperatures, showing > 70% spore germination even at temperatures as low as 5 °C. Less than 5% of spores obtained at 7 and 10 °C showed a germination response. Furthermore, spores produced at 12, 20 and 30 °C showed similar outgrowth effiency at these temperatures, indicating that low temperature sporulation history does not improve low temperature outgrowth performance. Insights obtained in sporulation and germination behaviour of B. weihenstephanensis KBAB4, in combination with the availability of its genome sequence, may contribute to our understanding of the behaviour of psychrotolerant spoilage and pathogenic Bacill
Review of catalytic systems and thermodynamics for the Guerbet condensation reaction and challenges for biomass valorization
The Guerbet condensation reaction is an alcohol coupling reaction that has been known for more than a century. Because of the increasing availability of bio-based alcohol feedstock, this reaction is of growing importance and interest in terms of value chains of renewable chemical and biofuel production. Due to the specific branching pattern of the alcohol products, the Guerbet reaction has many interesting applications. In comparison to their linear isomers, branched-chain Guerbet alcohols have extremely low melting points and excellent fluidity. This review provides thermodynamic insights and unravels the various mechanistic steps involved. A comprehensive overview of the homogeneous, heterogeneous and combined homogeneous and heterogeneous catalytic systems described in published reports and patents is also given. Technological considerations, challenges and perspectives for the Guerbet chemistry are discussed
The multi-thermal and multi-stranded nature of coronal rain
In this work, we analyse coordinated observations spanning chromospheric, TR
and coronal temperatures at very high resolution which reveal essential
characteristics of thermally unstable plasmas. Coronal rain is found to be a
highly multi-thermal phenomenon with a high degree of co-spatiality in the
multi-wavelength emission. EUV darkening and quasi-periodic intensity
variations are found to be strongly correlated to coronal rain showers.
Progressive cooling of coronal rain is observed, leading to a height dependence
of the emission. A fast-slow two-step catastrophic cooling progression is
found, which may reflect the transition to optically thick plasma states. The
intermittent and clumpy appearance of coronal rain at coronal heights becomes
more continuous and persistent at chromospheric heights just before impact,
mainly due to a funnel effect from the observed expansion of the magnetic
field. Strong density inhomogeneities on spatial scales of 0.2"-0.5" are found,
in which TR to chromospheric temperature transition occurs at the lowest
detectable scales. The shape of the distribution of coronal rain widths is
found to be independent of temperature with peaks close to the resolution limit
of each telescope, ranging from 0.2" to 0.8". However we find a sharp increase
of clump numbers at the coolest wavelengths and especially at higher
resolution, suggesting that the bulk of the rain distribution remains
undetected. Rain clumps appear organised in strands in both chromospheric and
TR temperatures, suggesting an important role of thermal instability in the
shaping of fundamental loop substructure. We further find structure reminiscent
of the MHD thermal mode. Rain core densities are estimated to vary between
2x10^{10} cm^{-3} and 2.5x10^{11} cm^{-3} leading to significant downward mass
fluxes per loop of 1-5x10^{9} g s^{-1}, suggesting a major role in the
chromosphere-corona mass cycle.Comment: Abstract is only short version. See paper for full. Countless pages,
figures (and movies, but not included here). Accepted for publication in the
Astrophysical Journa
Quantifying Spicules
Understanding the dynamic solar chromosphere is fundamental in solar physics.
Spicules are an important feature of the chromosphere, connecting the
photosphere to the corona, potentially mediating the transfer of energy and
mass. The aim of this work is to study the properties of spicules over
different regions of the sun. Our goal is to investigate if there is more than
one type of spicules, and how spicules behave in the quiet sun, coronal holes,
and active regions. We make use of high-cadence and high-spatial resolution Ca
II H observations taken by Hinode/SOT. Making use of a semi-automated detection
algorithm, we self-consistently track and measure the properties of 519
spicules over different regions. We find clear evidence of two types of
spicules. Type I spicules show a rise and fall and have typical lifetimes of
150-400 s and maximum ascending velocities of 15-40 km/s, while type II
spicules have shorter lifetimes of 50-150 s, faster velocities of 30-110 km/s,
and are not seen to fall down, but rather fade at around their maximum length.
Type II spicules are the most common, seen in quiet sun and coronal holes. Type
I spicules are seen mostly in active regions. There are regional differences
between quiet sun and coronal hole spicules, likely attributable to the
different field configurations. The properties of type II spicules are
consistent with published results of Rapid Blueshifted Events (RBEs),
supporting the hypothesis that RBEs are their disk counterparts. For type I
spicules we find the relations between their properties to be consistent with a
magnetoacoustic shock wave driver, and with dynamic fibrils as their disk
counterpart. The driver of type II spicules remains unclear from limb
observations.Comment: Accepted for publication in ApJ. 17 pages, 9 figure
Effect of the C-bridge length on the ultraviolet-resistance of oxycarbosilane low-k films
The ultra-violet (UV) and vacuum ultra-violet (VUV) resistance of bridging alkylene groups in organosilica films has been investigated. Similar to the Si-CH3 (methyl) bonds, the Si-CH2-Si (methylene) bonds are not affected by 5.6 eV irradiation. On the other hand, the concentration of the Si-CH2-CH2-Si (ethylene) groups decreases during such UV exposure. More significant difference in alkylene reduction is observed when the films are exposed to VUV (7.2 eV). The ethylene groups are depleted by more than 75% while only about 40% methylene and methyl groups loss is observed. The different sensitivity of bridging groups to VUV light should be taken into account during the development of curing and plasma etch processes of low-k materials based on periodic mesoporous organosilicas and oxycarbosilanes. The experimental results are qualitatively supported by ab-initio quantum-chemical calculations
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