703 research outputs found
Transcriptional Regulation of the Human Hepatic Lipase Gene: Relation to Glucose and Lipid Metabolism
Hepatic Lipase (HL; EC 3.1.1.3) is an extracellular glycoprotein with phospholipase A1 and
triacylglycerol hydrolase activity. The human HL protein is encoded by the LIPC gene on
chromosome 15q21. Most of this protein is synthesized in the parenchymal cells of the liver
and secreted into the space of Disse where it binds to heparin sulfate proteoglycans.
Some synthesis of HL was also observed in macrophages. The HL protein is also present in
the steroidogenic adrenal glands, ovaries and, in small amounts, in the testes. By using
heparin, HL protein is displaced from its binding site. Human HL protein is a homodimer, the
monomer has a molecular weight of 65 kDa. In the metabolism of plasma lipoproteins HL
plays an important role; it mediates the conversion of high density lipoprotein subfraction 2
(HDL2) to high density lipoprotein subfraction 3 (HDL3), the conversion of intermediate density
lipoprotein (IDL) to low density l
On the Effectiveness of Unit Tests in Test-driven Development
Background: Writing unit tests is one of the primary activities
in test-driven development. Yet, the existing reviews report few
evidence supporting or refuting the effect of this development approach
on test case quality. Lack of ability and skills of developers to
produce sufficiently good test cases are also reported as limitations
of applying test-driven development in industrial practice.
Objective: We investigate the impact of test-driven development
on the effectiveness of unit test cases compared to an incremental
test last development in an industrial context.
Method: We conducted an experiment in an industrial setting
with 24 professionals. Professionals followed the two development
approaches to implement the tasks. We measure unit test effectiveness
in terms of mutation score. We also measure branch and
method coverage of test suites to compare our results with the
literature.
Results: In terms of mutation score, we have found that the test
cases written for a test-driven development task have a higher
defect detection ability than test cases written for an incremental
test-last development task. Subjects wrote test cases that cover
more branches on a test-driven development task compared to the
other task. However, test cases written for an incremental test-last
development task cover more methods than those written for the
second task.
Conclusion: Our findings are different from previous studies
conducted at academic settings. Professionals were able to perform
more effective unit testing with test-driven development. Furthermore,
we observe that the coverage measure preferred in academic
studies reveal different aspects of a development approach. Our
results need to be validated in larger industrial contexts.Istanbul Technical University
Scientific Research Projects (MGA-2017-40712), and the
Academy of Finland (Decision No. 278354)
Defining Domain-Specific Modeling Languages to Automate Product Derivation: Collected Experiences
Abstract. Domain-Specific Modeling offers a language-based approach to raise the level of abstraction in order to speed up development work and set variation space already at specification and design phase. In this paper we identify approaches that are applied for defining languages that enable automated variant derivation. This categorization is based on analyzing over 20 industrial cases of DSM language definition.
Prototyping the Semantics of a DSL using ASF+SDF: Link to Formal Verification of DSL Models
A formal definition of the semantics of a domain-specific language (DSL) is a
key prerequisite for the verification of the correctness of models specified
using such a DSL and of transformations applied to these models. For this
reason, we implemented a prototype of the semantics of a DSL for the
specification of systems consisting of concurrent, communicating objects. Using
this prototype, models specified in the DSL can be transformed to labeled
transition systems (LTS). This approach of transforming models to LTSs allows
us to apply existing tools for visualization and verification to models with
little or no further effort. The prototype is implemented using the ASF+SDF
Meta-Environment, an IDE for the algebraic specification language ASF+SDF,
which offers efficient execution of the transformation as well as the ability
to read models and produce LTSs without any additional pre or post processing.Comment: In Proceedings AMMSE 2011, arXiv:1106.596
Fermi Surface Properties of Low Concentration CeLaB: dHvA
The de Haas-van Alphen effect is used to study angular dependent extremal
areas of the Fermi Surfaces (FS) and effective masses of CeLaB alloys for between 0 and 0.05. The FS of these alloys was previously
observed to be spin polarized at low Ce concentration ( = 0.05). This work
gives the details of the initial development of the topology and spin
polarization of the FS from that of unpolarized metallic LaB to that of
spin polarized heavy Fermion CeB .Comment: 7 pages, 9 figures, submitted to PR
Heavy quasiparticles in the ferromagnetic superconductor ZrZn2
We report a study of the de Haas-van Alphen effect in the normal state of the
ferromagnetic superconductor ZrZn2. Our results are generally consistent with
an LMTO band structure calculation which predicts four exchange-split Fermi
surface sheets. Quasiparticle effective masses are enhanced by a factor of
about 4.9 implying a strong coupling to magnetic excitations or phonons. Our
measurements provide insight in to the mechanism for superconductivity and
unusual thermodynamic properties of ZrZn2.Comment: 5 pages, 2 figures (one color
The epigenetic regulators CBP and p300 facilitate leukemogenesis and represent therapeutic targets in acute myeloid leukemia.
Growing evidence links abnormal epigenetic control to the development of hematological malignancies. Accordingly, inhibition of epigenetic regulators is emerging as a promising therapeutic strategy. The acetylation status of lysine residues in histone tails is one of a number of epigenetic post-translational modifications that alter DNA-templated processes, such as transcription, to facilitate malignant transformation. Although histone deacetylases are already being clinically targeted, the role of histone lysine acetyltransferases (KAT) in malignancy is less well characterized. We chose to study this question in the context of acute myeloid leukemia (AML), where, using in vitro and in vivo genetic ablation and knockdown experiments in murine models, we demonstrate a role for the epigenetic regulators CBP and p300 in the induction and maintenance of AML. Furthermore, using selective small molecule inhibitors of their lysine acetyltransferase activity, we validate CBP/p300 as therapeutic targets in vitro across a wide range of human AML subtypes. We proceed to show that growth retardation occurs through the induction of transcriptional changes that induce apoptosis and cell-cycle arrest in leukemia cells and finally demonstrate the efficacy of the KAT inhibitors in decreasing clonogenic growth of primary AML patient samples. Taken together, these data suggest that CBP/p300 are promising therapeutic targets across multiple subtypes in AML.Funding in the Huntly laboratory comes from Cancer Research UK, Leukemia
Lymphoma Research, the Kay Kendal Leukemia Fund, the Leukemia lymphoma
Society of America, the Wellcome Trust, The Medical Research Council and an NIHR
Cambridge Biomedical Research Centre grant. Patient samples were processed in the
Cambridge Blood and Stem Cell Biobank.This is the author accepted manuscript. The final version is available via NPG at http://dx.doi.org/10.1038/onc.2015.9
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