55 research outputs found
Lower NPAS3 expression during the later stages of abnormal lung development in rat congenital diaphragmatic hernia
Purpose
Congenital diaphragmatic hernia (CDH) is characterized by a developmental defect in the diaphragm, pulmonary hypoplasia and pulmonary hypertension. NPAS3 is a PAS domain transcription factor regulating Drosophila tracheogenesis. NPAS3 null mice develop pulmonary hypoplasia in utero and die after birth due to respiratory failure. We aimed to evaluate NPAS3 expres- sion during normal and abnormal lung development due to CDH.
Methods
CDH was induced by administering 100 mg/ml nitrofen to time-pregnant dams on embryonic day (E) 9 of gestation. Lungs were isolated on E15, E18 and E21 and NPAS3 localization was determined by immunohisto- chemistry and quantified using Western blotting.
Results
We found that only E21 hypoplastic CDH lungs have reduced expression of NPAS3 in the terminal sac- cules. Western blotting confirmed the down-regulation of NPAS3 protein in the nitrofen-induced hypoplastic lungs.
Conclusions
We demonstrate for the first time that ni- trofen-induced hypoplastic CDH lungs have reduced NPAS3 expression in the terminal saccules during the later stages of abnormal lung development. Our findings suggest that NPAS3 is associated with pulmonary hypoplasia in CDH.Supported by the Children’s Hospital Research Institute of Manitoba; RK is the recipient of a Career Enhancement Award from the Canadian Child Health Clinician Scientist Program and a New Investigator Salary Award from the Canadian Institutes of Health Research, Manitoba Lung Association and the Children’s Hospital Research Institute
Switchable Gene Expression in Escherichia coli Using a Miniaturized Photobioreactor
We present a light-switchable gene expression system for both inducible and switchable control of gene expression at a single cell level in Escherichia coli using a previously constructed light-sensing system. The lambda cl repressor gene with an LVA degradation tag was expressed under the control of the ompC promoter on the chromosome. The green fluorescent protein (GFP) gene fused to a lambda repressor-repressible promoter was used as a reporter. This light-switchable system allows rapid and reversible induction or repression of expression of the target gene at any desired time. This system also ensures homogenous expression across the entire cell population. We also report the design of a miniaturized photobioreactor to be used in combination with the light-switchable gene expression system. The miniaturized photobioreactor helps to reduce unintended induction of the light receptor due to environmental disturbances and allows precise control over the duration of induction. This system would be a good tool for switchable, homogenous, strong, and highly regulatable expression of target genes over a wide range of induction times. Hence, it could be applied to study gene function, optimize metabolic pathways, and control biological systems both spatially and temporally.open0
Study of in the vicinity of
Using 2917 of data accumulated at 3.773~,
44.5~ of data accumulated at 3.65~ and data accumulated
during a line-shape scan with the BESIII detector, the reaction
is studied considering a possible interference
between resonant and continuum amplitudes. The cross section of
,
, is found to have two
solutions, determined to be () pb with the phase angle
(0.11 pb at the 90% confidence level),
or ) pb with both of which
agree with a destructive interference. Using the obtained cross section of
, the cross section of , which is useful information for the future PANDA experiment, is
estimated to be either () nb ( nb at 90% C.L.) or
nb
Transfer function development for control of cathode airflow transients in fuel cell gas turbine hybrid systems
Direct-fired fuel cell gas turbine hybrid power system responses to open-loop transients were evaluated using a hardware-based simulation of an integrated solid oxide fuel cell gas turbine (SOFC/GT) hybrid system, implemented through the Hybrid Performance (Hyper) facility at the U.S. Department of Energy, National Energy Technology Laboratory (NETL). A disturbance in the cathode inlet air mass flow was performed by manipulating a hot-air bypass valve implemented in the hardware component. Two tests were performed; the fuel cell stack subsystem numerical simulation model was both decoupled and fully coupled with the gas turbine hardware component. The dynamic responses of the entire SOFC/GT hybrid system were studied in this paper. The reduction of cathode airflow resulted in a sharp decrease and partial recovery of the fuel cell thermal effluent in 10 s. In contrast, the turbine rotational speed did not exhibit a similar trend. The transfer functions of several important variables in the fuel cell stack subsystem and gas turbine subsystem were developed to be used in the future control method development. The importance of the cathode airflow regulation was quantified through transfer functions. The management of cathode airflow was also suggested to be a potential strategy to increase the life of fuel cells by reducing the thermal impact of operational transients on the fuel cell subsystem
Performance analysis of network traffic capture tools and machine learning algorithms for the classification of applications, states and anomalies
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Microbial Functional Gene Diversity with a Shift of Subsurface Redox Conditions during In Situ Uranium Reduction
To better understand the microbial functional diversity changes with subsurface redox conditions during in situ uranium bioremediation, key functional genes were studied with GeoChip, a comprehensive functional gene microarray, in field experiments at a uranium mill tailings remedial action (UMTRA) site (Rifle, CO). The results indicated that functional microbial communities altered with a shift in the dominant metabolic process, as documented by hierarchical cluster and ordination analyses of all detected functional genes. The abundance of dsrAB genes (dissimilatory sulfite reductase genes) and methane generation-related mcr genes (methyl coenzyme M reductase coding genes) increased when redox conditions shifted from Fe-reducing to sulfate-reducing conditions. The cytochrome genes detected were primarily from Geobacter sp. and decreased with lower subsurface redox conditions. Statistical analysis of environmental parameters and functional genes indicated that acetate, U(VI), and redox potential (Eh) were the most significant geochemical variables linked to microbial functional gene structures, and changes in microbial functional diversity were strongly related to the dominant terminal electron-accepting process following acetate addition. The study indicates that the microbial functional genes clearly reflect the in situ redox conditions and the dominant microbial processes, which in turn influence uranium bioreduction. Microbial functional genes thus could be very useful for tracking microbial community structure and dynamics during bioremediation
“Benign intracranial hypertension” is a misleading name for a more serious disease: analysis of the visual outcome in cases with idiopathic intracranial hypertension
First Characterization of PAH-degrading bacteria from Río de la Plata and high-resolution melting: an encouraging step toward bioremediation
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