U.S. Population, Energy & Climate Change

Explains how U.S. population trends tend to exacerbate both the causes and effects of climate change. Outlines how population density and composition affect energy and land use, the role each U.S. region plays in climate change, and the risks they face

Lipid Coated Gold Nanoparticle Cores: Synthesis and Characterization

Including environmental, industrial, and biomedical sciences, applications of gold nanoparticles are on the forefront of research in many areas. By altering the surface treatment of spherical gold nanoparticle cores, particularly those smaller than 100 nm (nanometers), one can influence their potential use in a number of ways. Lipid coated nanoparticles with specifically selected surface ligands can be used for multiple biomedical functions, including medical imaging, for use as colorimetric and plasmonic sensors within the body, and as cell or organelle specific targets for therapeutic drug delivery or cancer treatment. Here, spherical gold nanoparticles ranging in size from 8-40 nm (avg. diameter 23-48 nm) have been synthesized and coated with poly(allylamine hydrochloride) (PAH) and a mixed lipid solution of 1:1 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) and lysophosphatidylcholine (LPC), two of the four major types of lipids found in the human body. Characterization was performed using a NanoSight LM10HS particle sizer, and shows a gradual increase in size after each step in the coating process for nanoparticle cores ranging in size from 16-27 nm. The thickness of these purified and lipid coated nanoparticles was consistently 2-3 times that of the PAH coated sample it was layered onto, suggesting a successful, multi-layered coat that ranges in size based on the PAH coated core size. UV-Vis spectroscopy shows a slight red shift, indicating an increase in size and change in refractive index, which supports the presence of lipid coating on the PAH coated gold nanoparticle cores

microRNAs of parasitic helminths – identification, characterization and potential as drug targets

microRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional gene regulation. They were first identified in the free-living nematode Caenorhabditis elegans, where the miRNAs lin-4 and let-7 were shown to be essential for regulating correct developmental progression. The sequence of let-7 was subsequently found to be conserved in higher organisms and changes in expression of let-7, as well as other miRNAs, are associated with certain cancers, indicating important regulatory roles. Some miRNAs have been shown to have essential functions, but the roles of many are currently unknown. With the increasing availability of genome sequence data, miRNAs have now been identified from a number of parasitic helminths, by deep sequencing of small RNA libraries and bioinformatic approaches. While some miRNAs are widely conserved in a range of organisms, others are helminth-specific and many are novel to each species. Here we review the potential roles of miRNAs in regulating helminth development, in interacting with the host environment and in development of drug resistance. Use of fluorescently-labeled small RNAs demonstrates uptake by parasites, at least in vitro. Therefore delivery of miRNA inhibitors or mimics has potential to alter miRNA activity, providing a useful tool for probing the roles of miRNAs and suggesting novel routes to therapeutics for parasite control

Identifying prognostic indicators for electrical treeing in solid insulation through pulse sequence analysis

Predictive maintenance attempts to evaluate the condition of equipment and predict the future trend of the equipment's aging, in order to reduce costs when compared to the two traditional approaches: corrective and preventive maintenance. This prediction requires an accurate prognostic model of aging. In solid insulation, the ultimate goal of prognostics is to predict the advent of failure, i.e., insulation breakdown, in terms of remaining useful life (RUL). One fault is electrical treeing, which is progressive thus leading to potentially catastrophic failure. Research has shown that diagnosis of faults can be achieved based on partial discharge (PD) monitoring [1], i.e., phase-resolved and pulse sequence analysis (PSA). This work will explore the extension of this concept towards predicting evolution of the defect: moving beyond diagnostics towards prognostics. To do this, there is a need for further investigation of prognostic features within PD characteristics leading up to breakdown. In this work, a needle-plane test arrangement was set up using a hypodermic needle and pre-formed silicone rubber as test samples. The visual observations and tree growth measurements were made using a digital microscope. PD data was captured using a radio frequency (RF) sensor and analysed using PSA. The main idea of the PSA approach is the strong relationship between two consecutive pulses caused by PD activities, which can give an understanding of the local degradation processes [1]. As for electrical treeing, a breakdown indicator in PSA is the appearance of heavily clustered data points that lie diagonally in scatter plots of the differential ratio of voltage and time of consecutive charges (Un = Δun/Δtn) [2,3]. Figure 1 shows an example of a plot that changed to a diagonal line after 14 hours of aging time. This paper investigates the formation of the diagonal line based on the distribution of the plot from the start of electrical treeing until breakdown occurs. Finally, statistical features of the PSA plot are given and will be used for lifetime prediction of insulation samples in future work

Developing a computer aided design tool for inclusive design

The purpose of this study was to investigate age-related changes in the performance of a range of movement tasks for integration into a computer aided design (CAD) tool for use in inclusive design

Organic Haze as a Biosignature in Anoxic Earth-like Atmospheres

Early Earth may have hosted a biologically-mediated global organic haze during the Archean eon (3.8-2.5 billion years ago). This haze would have significantly impacted multiple aspects of our planet, including its potential for habitability and its spectral appearance. Here, we model worlds with Archean-like levels of carbon dioxide orbiting the ancient sun and an M4V dwarf (GJ 876) and show that organic haze formation requires methane fluxes consistent with estimated Earth-like biological production rates. On planets with high fluxes of biogenic organic sulfur gases (CS2, OCS, CH3SH, and CH3SCH3), photochemistry involving these gases can drive haze formation at lower CH4/CO2 ratios than methane photochemistry alone. For a planet orbiting the sun, at 30x the modern organic sulfur gas flux, haze forms at a CH4/CO2 ratio 20% lower than at 1x the modern organic sulfur flux. For a planet orbiting the M4V star, the impact of organic sulfur gases is more pronounced: at 1x the modern Earth organic sulfur flux, a substantial haze forms at CH4/CO2 ~ 0.2, but at 30x the organic sulfur flux, the CH4/CO2 ratio needed to form haze decreases by a full order of magnitude. Detection of haze at an anomalously low CH4/CO2 ratio could suggest the influence of these biogenic sulfur gases, and therefore imply biological activity on an exoplanet. When these organic sulfur gases are not readily detectable in the spectrum of an Earth-like exoplanet, the thick organic haze they can help produce creates a very strong absorption feature at UV-blue wavelengths detectable in reflected light at a spectral resolution as low as 10. In direct imaging, constraining CH4 and CO2 concentrations will require higher spectral resolution, and R > 170 is needed to accurately resolve the structure of the CO2 feature at 1.57 {\mu}m, likely, the most accessible CO2 feature on an Archean-like exoplanet.Comment: accepted for publication in Astrobiolog

Frequency over function : raised levels of CD127low/- regulatory T cells in the tumour microenvironment compared with the periphery of head and neck cancer patients

Objective: Regulatory T cells (Tregs) are known to infiltrate the tumour microenvironment of many cancers, including head and neck malignancies, and are thought to contribute to the host's impaired anti-tumour immune response. However, their immunosuppressive function remains poorly understood within the tumour microenvironment and this study aimed to address this. Methods: The frequency and suppressive capacity of two CD4?CD127low/- Treg populations, separated on the basis of different levels of CD25 expression (CD25inter and CD25high), from the tumour/node microenvironment and peripheral circulation of newly-presenting head and neck squamous cell carcinoma patients (n=19), were assessed using multicolour flow cytometry. Results: The proportion of Tregs (CD4?CD25high/?interCD127low/-) in the tumour/node microenvironment was significantly elevated compared with the peripheral circulation (p<0.001) and similar percentages were present in both the primary tumour and metastatic lymph node. The percentage of suppression induced by Tregs isolated from tumour associated nodes on the proliferation of nodal effector T cells was similar to that of peripheral Tregs on peripheral effector T cells. However, when the suppressive activity of both nodal and peripheral Tregs was compared on the same peripheral effectors, peripheral Tregs suppressed proliferation to a greater extent. Conclusion: This work shows that the recruitment and percentages of tumour infiltrating Tregs are key factors in modulating the immune environment of head and neck tumours

Asymmetries in top quark pair production at hadron colliders

We review the asymmetries in top quark pair production at the Tevatron and the LHC. We summarize the experimental measurements and the interpretations of a possible excess in terms of new physics. We also review other top quark properties-emphasizing effects related to the $t \bar t$ asymmetries-as well as other collider signals.Comment: RevTeX 40 pages. Final version to be published in Reviews of Modern Physics, with several addition