Implementation of fatigue model for unidirectional laminate based on finite element analysis : theory and practice

The aim of this study is to deal with the simulation of intra-laminar fatigue damage in unidirectional composite under multi-axial and variable amplitude loadings. The variable amplitude and multi-axial loading is accounted for by using the damage hysteresis operator based on Brokate method [6]. The proposed damage model for fatigue is based on stiffness degradation laws from Van Paepegem combined with the 'damage' cycle jump approach extended to deal with unidirectional carbon fibres. The parameter identification method is here presented and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the LadevSze damage model and the permanent shear strain accumulation based on Van Paepegem's formulation. This approach is implemented into commercial software (Siemens PLM). The validation case is run on a bending test coupon (with arbitrary stacking sequence and load level) in order to minimise the risk of inter-laminar damages. This intra-laminar fatigue damage model combined efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level. This work concludes on the next challenges to be addressed (validation tests, multiple-loadings validation, failure criteria, inter-laminar damages...)

A progessive damage fatigue model for unidirectional laminated composites based on finite element analysis: theory and practice

The simulation of the fatigue damage of laminated composites under multi-axial and variable amplitude loadings has to deal with several new challenges and several methods of damage modelling. In this paper we present how to account for the complex loading by using the damage hysteresis operator approach for fatigue. It is applied to a fatigue model for intra-laminar damage based on stiffness degradation laws from Van Paepegem and has been extended to deal with unidirectional carbon fibres. The parameter identification method is presented here and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the Ladevèze damage model and the permanent shear strain accumulation based on Van Paepegem’s formulation. This approach has been implemented into commercial software. The intra-laminar fatigue damage model combines efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level

A progessive damage fatigue model for unidirectional laminated composites based on finite element analysis: theory and practice

The simulation of the fatigue damage of laminated composites under multi-axial and variable amplitude loadings has to deal with several new challenges and several methods of damage modelling. In this paper we present how to account for the complex loading by using the damage hysteresis operator approach for fatigue. It is applied to a fatigue model for intra-laminar damage based on stiffness degradation laws from Van Paepegem and has been extended to deal with unidirectional carbon fibres. The parameter identification method is presented here and parameter sensitivities are discussed. The initial static damage of the material is accounted for by using the Ladevèze damage model and the permanent shear strain accumulation based on Van Paepegem’s formulation. This approach has been implemented into commercial software. The intra-laminar fatigue damage model combines efficient methods with a low number of tests to identify the parameters of the stiffness degradation law, this overall procedure for fatigue life prediction is demonstrated to be cost efficient at industrial level

RNA metabolism is the primary target of formamide in vivo

The synthesis, processing and function of coding and non-coding RNA molecules and their interacting proteins has been the focus of a great deal of research that has boosted our understanding of key molecular pathways that underlie higher order events such as cell cycle control, development, innate immune response and the occurrence of genetic diseases. In this study, we have found that formamide preferentially weakens RNA related processes in vivo. Using a non-essential Schizosaccharomyces pombe gene deletion collection, we identify deleted loci that make cells sensitive to formamide. Sensitive deletions are significantly enriched in genes involved in RNA metabolism. Accordingly, we find that previously known temperature-sensitive splicing mutants become lethal in the presence of the drug under permissive temperature. Furthermore, in a wild type background, splicing efficiency is decreased and R-loop formation is increased in the presence of formamide. In addition, we have also isolated 35 formamide-sensitive mutants, many of which display remarkable morphology and cell cycle defects potentially unveiling new players in the regulation of these processes. We conclude that formamide preferentially targets RNA related processes in vivo, probably by relaxing RNA secondary structures and/or RNA-protein interactions, and can be used as an effective tool to characterize these processes

A connection between pre-mRNA splicing and the cell cycle in fission yeast: cdc28+ is allelic with prp8+ and encodes an RNA-dependent ATPase/helicase.

The fission-yeast gene cdc28+ was originally identified in a screen for temperature-sensitive mutants that exhibit a cell-division cycle arrest and was found to be required for mitosis. We undertook a study of this gene to understand more fully the general requirements for entry into mitosis. Cells carrying the conditional lethal cdc28-P8 mutation divide once and arrest in G2 after being shifted to the restrictive temperature. We cloned the cdc28+ gene by complementation of the temperature-sensitive growth arrest in cdc28-P8. DNA sequence analysis indicated that cdc28+ encodes a member of the DEAH-box family of putative RNA-dependent ATPases or helicases. The Cdc28 protein is most similar to the Prp2, Prp16, and Prp22 proteins from budding yeast, which are required for the splicing of mRNA precursors. Consistent with this similarity, the cdc28-P8 mutant accumulates unspliced precursors at the restrictive temperature. Independently, we isolated a temperature-sensitive pre-mRNA splicing mutant prp8-1 that exhibits a cell-cycle phenotype identical to that of cdc28-P8. We have shown that cdc28 and prp8 are allelic. These results suggest a connection between pre-mRNA splicing and progression through the cell cycle

The prediction of low- and mid-frequency internal road vehicle noise: a literature survey

Over the past 40 years the low- and mid-frequency internal noise of road vehiles has been of increasing interest to both manufacturers and customers, and there have been many papers written on the subject. It is particularly important that manufacturers are able to predict the noise at an early stage of a new design so that expensive mistakes can be avoided. This paper reviews the relevant literature published over this 40 year period and concludes that the finite element method (FEM), and/or the boundary element method (BEM) are currently the most accurate ways of predicting this noise. However, although the emphasis of this review is on the low- and mid-frequency structure-borne aspect of the noise, other prediction methods (which are normally considered to be only applicable at high frequencies) are also considered. In particular, the statistical energy analysis (SEA) is shown to be an increasingly useful tool for predicting structure-borne noise, as is the newly developed FEM/SEA hybrid method. Other essentially high-frequency techniques are also considered in this review because recent research indicates that it might be possible to apply these methods over a broader frequency range than was initially envisaged