STUDY OF THE LIPID COMPOSITION OF SOME PLANTS GROWING IN GEORGIA

Sums of neutral and polar lipids were extracted from the fruits of Vitis vinifera and pericarpium of Physalis alkekengi L.; their main classes and physical-chemical constants of some neutral lipids were determined. The saturated, unsaturated and polyunsaturated fatty acids were identified by using High Performance Liquid Chromatography. The phospholipids- lysophosphatidylcholine, phosphatidylinosite, phosphatidylcholine, phosphatidylethanolamine and N-acyl phosphatidylethanolamine were identified in them. The content of carotenoids is up 2,5 mg% and 65,5mg% in Vitis vinifera and Physalis alkekengi L. respectively. Amino acids were detected in studied species. The oils from both plants are characterized with anti-inflammatory, hepatoprotective, choleretic and immunotropic activities, they can be used in medicine for curative and preventive purposes.Sums of neutral and polar lipids were extracted from the fruits of Vitis vinifera and pericarpium of Physalis alkekengi L.; their main classes and physical-chemical constants of some neutral lipids were determined. The saturated, unsaturated and polyunsaturated fatty acids were identified by using High Performance Liquid Chromatography. The phospholipids- lysophosphatidylcholine, phosphatidylinosite, phosphatidylcholine, phosphatidylethanolamine and N-acyl phosphatidylethanolamine were identified in them. The content of carotenoids is up 2,5 mg% and 65,5mg% in Vitis vinifera and Physalis alkekengi L. respectively. Amino acids were detected in studied species. The oils from both plants are characterized with anti-inflammatory, hepatoprotective, choleretic and immunotropic activities, they can be used in medicine for curative and preventive purposes

Molecular characterization and antiviral activity test of common drugs against echovirus 18 isolated in Korea

Genetic diversity and antiviral activity for five common antiviral drugs of echovirus (ECV) 5 isolated in Korea have been described. The present study extended these tests to a Korean ECV 18 isolate. An outbreak of aseptic meningitis caused by the ECV 18 isolate was reported in Korea in 2005, marking the first time this virus had been identified in the country since enterovirus surveillance began in 1993. Using a sample isolated from stool specimen of a 5-year-old male patient with aseptic meningitis, the complete genome sequence was obtained and was compared it with the Metcalf prototype strain. Unlike the ECV5 isolate, the 3' untranslated region had the highest identity value (94.2%) at the nucleotide level, while, at the amino acid level, the P2 region displayed the highest identity value (96.9%). These two strains shared all cleavage sites, with the exception of the 2B/2C site, which was RQ/NN in the Metcalf strain but RQ/NS in the Korean ECV 18 isolate. In Vero cells infected with the Korean ECV 18 isolate, no cytotoxicity was observed in the presence of azidothymidine, acyclovir, amantadine, lamivudine, or ribavirin, when the drugs were administered at a CC50 value >100 μg/mL. Of the five drugs, only amantadine (IC50: 4.97 ± 0.77 μg/mL, TI: 20.12) and ribavirin (IC50: 7.63 ± 0.87 μg/mL, TI: 13.11) had any antiviral activity against the Korean ECV 18 isolate in the five antiviral drugs. These antiviral activity effects were similar with results of the Korean ECV5 isolate

Study Of The Lipid Composition Of Some Plants Growing In Georgia

Sums of neutral and polar lipids were extracted from the fruits of Vitis vinifera and pericarpium of Physalis alkekengi L.; their main classes and physical-chemical constants of some neutral lipids were determined. The saturated, unsaturated and polyunsaturated fatty acids were identified by using High Performance Liquid Chromatography. The phospholipids- lysophosphatidylcholine, phosphatidylinosite, phosphatidylcholine, phosphatidylethanolamine and N-acyl phosphatidylethanolamine were identified in them. The content of carotenoids is up 2,5 mg% and 65,5mg% in Vitis vinifera and Physalis alkekengi L. respectively. Amino acids were detected in studied species. The oils from both plants are characterized with anti-inflammatory, hepatoprotective, choleretic and immunotropic activities, they can be used in medicine for curative and preventive purposes

Outbreak of Shiga toxin-producing Escherichia coli (STEC) O157:H7 associated with romaine lettuce consumption, 2011.

BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is the causal agent for more than 96,000 cases of diarrheal illness and 3,200 infection-attributable hospitalizations annually in the United States. MATERIALS AND METHODS: We defined a confirmed case as a compatible illness in a person with the outbreak strain during 10/07/2011-11/30/2011. Investigation included hypothesis generation, a case-control study utilizing geographically-matched controls, and a case series investigation. Environmental inspections and tracebacks were conducted. RESULTS: We identified 58 cases in 10 states; 67% were hospitalized and 6.4% developed hemolytic uremic syndrome. Any romaine consumption was significantly associated with illness (matched Odds Ratio (mOR) = 10.0, 95% Confidence Interval (CI) = 2.1-97.0). Grocery Store Chain A salad bar was significantly associated with illness (mOR = 18.9, 95% CI = 4.5-176.8). Two separate traceback investigations for romaine lettuce converged on Farm A. Case series results indicate that cases (64.9%) were more likely than the FoodNet population (47%) to eat romaine lettuce (p-value = 0.013); 61.3% of cases reported consuming romaine lettuce from the Grocery Store Chain A salad bar. CONCLUSIONS: This multistate outbreak of STEC O157:H7 infections was associated with consumption of romaine lettuce. Traceback analysis determined that a single common lot of romaine lettuce harvested from Farm A was used to supply Grocery Store Chain A and a university campus linked to a case with the outbreak strain. An investigation at Farm A did not identify the source of contamination. Improved ability to trace produce from the growing fields to the point of consumption will allow more timely prevention and control measures to be implemented

Frequency of selected case-series exposures in case patients vs. FoodNet population survey exposure.

†<p>Any consumption in the seven days prior to illness onset.</p>‡<p>Foodborne Diseases Active Surveillance Network (FoodNet) Population Survey Atlas of Exposures, 2006–2007 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055300#pone.0055300-Centers1" target="_blank">[6]</a>.</p