An industrial survey on the use of surface texture parameters

In 1999, CIRP conducted an industrial survey of the use of surface texture parameters [1]. In the seventeen years since, much has changed, with the most important advancement being the introduction of areal surface texture parameters as described in ISO 25178-2 [2]. There has also been the release of commercial software packages for the calculation of surface texture parameters and, therefore, it is expected that industry is starting to embrace areal surface texture characterisation. Industry is also increasingly using more optical instruments, which are often inherently areal in nature. These factors bring to light the need for a new parameter survey, to investigate whether industry really has been adopting areal surface texture parameters. This study used an online survey to obtain information about the current use of surface texture parameters in industry. The survey features both profile and areal surface texture parameters defined in specification standards ISO 4287 [3], ISO 25178-2 [2], ISO 12085 [4] and ISO 16565-2/3 [5, 6]. The survey was open to responses for eight months and obtained a total of 179 responses from a variety of industrial users of surface texture parameters spread across thirty-two countries. Responses from the survey offer information about the usage of individual surface texture parameters, highlighting any parameters that are unpopular and may require attention. The survey also enables participants to share their opinion on the current range of parameters in use, giving an insight into the perception of surface texture parameters in industry. The results from the survey highlight a strong adoption by industry of the areal surface texture parameters defined in ISO 25178-2. In comparison to the 1999 survey, there has also been an overall increase in the use of profile surface texture parameters, and an increase in the variety of parameters used, particularly for the ISO 4287 roughness parameters, suggesting a better understanding of the range of parameters available and their uses. Conversely, this increase in parameter variety could be due to the greater computational power available to users of surface texture parameters, allowing them to use more parameters with little cost. The results of the surface texture parameter survey will serve as an indication of the current state of the industry to those interested in the widespread acceptance and evolution of surface texture parameters. The analysis of the survey will identify common potential improvement areas in surface texture parameter selection and provide a starting point from which to better promote the current selection and better educate the users

Results of an industrial survey on the use of surface texture parameters

In 1999, CIRP conducted an industrial survey of the use of surface texture parameters [1]. In the seventeen years since, much has changed, with the most important advancement being the introduction of areal surface texture parameters as described in ISO 25178-2 [2]. There has also been the release of commercial software packages for the calculation of surface texture parameters and, therefore, it is expected that industry is starting to embrace areal surface texture characterisation. Industry is also increasingly using more optical instruments, which are often inherently areal in nature. These factors bring to light the need for a new parameter survey, to investigate whether industry really has been adopting areal surface texture parameters. This study used an online survey to obtain information about the current use of surface texture parameters in industry. The survey features both profile and areal surface texture parameters defined in specification standards ISO 4287 [3], ISO 25178-2 [2], ISO 12085 [4] and ISO 13565-2/3 [5, 6]. The survey was open to responses for eight months and obtained a total of 179 responses from a variety of industrial users of surface texture parameters spread across thirty-two countries. Responses from the survey offer information about the usage of individual surface texture parameters, highlighting any parameters that are unpopular and may require attention. The survey also enables participants to share their opinion on the current range of parameters in use, giving an insight into the perception of surface texture parameters in industry. The results from the survey highlight a strong adoption by industry of the areal surface texture parameters defined in ISO 25178-2. In comparison to the 1999 survey, there has also been an overall increase in the use of profile surface texture parameters, and an increase in the variety of parameters used, particularly for the ISO 4287 roughness parameters, suggesting a better understanding of the range of parameters available and their uses. Conversely, this increase in parameter variety could be due to the greater computational power available to users of surface texture parameters, allowing them to use more parameters with little cost. The results of the surface texture parameter survey will serve as an indication of the current state of the industry to those interested in the widespread acceptance and evolution of surface texture parameters. The analysis of the survey will identify common potential improvement areas in surface texture parameter selection and provide a starting point from which to better promote the current selection and better educate the users

Development and validation of a chemostat gut model to study both planktonic and biofilm modes of growth of Clostridium difficile and human microbiota

Copyright: 2014 Crowther et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.The human gastrointestinal tract harbours a complex microbial community which exist in planktonic and sessile form. The degree to which composition and function of faecal and mucosal microbiota differ remains unclear. We describe the development and characterisation of an in vitro human gut model, which can be used to facilitate the formation and longitudinal analysis of mature mixed species biofilms. This enables the investigation of the role of biofilms in Clostridium difficile infection (CDI). A well established and validated human gut model of simulated CDI was adapted to incorporate glass rods that create a solid-gaseous-liquid interface for biofilm formation. The continuous chemostat model was inoculated with a pooled human faecal emulsion and controlled to mimic colonic conditions in vivo. Planktonic and sessile bacterial populations were enumerated for up to 46 days. Biofilm consistently formed macroscopic structures on all glass rods over extended periods of time, providing a framework to sample and analyse biofilm structures independently. Whilst variation in biofilm biomass is evident between rods, populations of sessile bacterial groups (log10 cfu/g of biofilm) remain relatively consistent between rods at each sampling point. All bacterial groups enumerated within the planktonic communities were also present within biofilm structures. The planktonic mode of growth of C. difficile and gut microbiota closely reflected observations within the original gut model. However, distinct differences were observed in the behaviour of sessile and planktonic C. difficile populations, with C. difficile spores preferentially persisting within biofilm structures. The redesigned biofilm chemostat model has been validated for reproducible and consistent formation of mixed species intestinal biofilms. This model can be utilised for the analysis of sessile mixed species communities longitudinally, potentially providing information of the role of biofilms in CDI.Peer reviewe

Industrial survey of ISO surface texture parameters

Results of an international survey are presented, detailing the use of surface texture parameters in industry. The survey received 179 responses from a total of 34 countries, revealing the use of a variety of parameters from ISO 4287, ISO 12085, ISO 13565-2/3 and ISO 25178-2. The survey responses show an increase in the number of users of profile parameters, and an increase in the range of surface texture parameters used, compared to the results from a similar survey in 1999, as well as a significant uptake of the new areal surface texture parameters. Individual sector usage is also discussed

A programmable software framework for the generation of simulated surface topography

A flexible and programmable software framework has been developed that enables the creation of simulated, areal surface topography datasets. The framework allows the creation of surfaces through a layered approach where deterministic topographic structures can be combined with pseudo-random periodic and non-periodic components. The software can be used to generate reference topographies useful for the testing and validation of surface metrology methods and algorithms. The software framework is implemented in Matlab, and features a graphical user interface that enables easy navigation, and allows users to control the topography creation process. In addition to providing a complete analytical description for some classes of generated surfaces, the framework allows the surface datasets to be exported in the Surface Data File format, thus enabling easy transfer to a wide array of commercial surface metrology software applications

Drooling Reduction Intervention randomised trial (DRI): comparing the efficacy and acceptability of hyoscine patches and glycopyrronium liquid on drooling in children with neurodisability

Objective: Investigate whether hyoscine patch or glycopyrronium liquid is more effective and acceptable to treat drooling in children with neurodisability. Design: Multicentre, single-blind, randomised controlled trial. Setting: Recruitment through neurodisability teams; treatment by parents. Participants: Ninety children with neurodisability who had never received medication for drooling (55 boys, 35 girls; median age 4 years). Exclusion criteria: medication contraindicated; in a trial that could affect drooling or management. Intervention: Children were randomised to receive a hyoscine skin patch or glycopyrronium liquid. Dose was increased over 4 weeks to achieve optimum symptom control with minimal side-effects; steady dose then continued to 12 weeks. Primary and secondary outcomes: Primary outcome: Drooling Impact Scale (DIS) score at week-4. Secondary outcomes: change in DIS scores over 12 weeks, Drooling Severity and Frequency Scale and Treatment Satisfaction Questionnaire for Medication; adverse events; children’s perception about treatment. Results: Both medications yielded clinically and statistically significant reductions in mean DIS at week-4 (25.0 (SD 22.2) for hyoscine and 26.6 (SD 16) for glycopyrronium). There was no significant difference in change in DIS scores between treatment groups. By week-12, 26/47 (55%) children starting treatment were receiving hyoscine compared with 31/38 (82%) on glycopyrronium. There was a 42% increased chance of being on treatment at week-12 for children randomised to glycopyrronium relative to hyoscine (1.42, 95% CI 1.04 to 1.95). Conclusions: Hyoscine and glycopyrronium are clinically effective in treating drooling in children with neurodisability. Hyoscine produced more problematic side effects leading to a greater chance of treatment cessation

SMT19969 as a treatment for Clostridium difficile infection : an assessment of antimicrobial activity using conventional susceptibility testing and an in vitro gut model

© The Author 2014. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/ 4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.OBJECTIVES: We investigated the efficacy of the novel antimicrobial agent SMT19969 in treating simulated Clostridium difficile infection using an in vitro human gut model. METHODS: Concentrations of the predominant cultivable members of the indigenous gut microfloras and C. difficile (total and spore counts) were determined by viable counting. Cytotoxin titres were determined using cell cytotoxicity and expressed as log10 relative units (RU). Clindamycin was used to induce simulated C. difficile PCR ribotype 027 infection. Once high-level cytotoxin titres (≥ 4 RU) were observed, SMT19969 was instilled for 7 days. Two SMT19969 dosing regimens (31.25 and 62.5 mg/L four times daily) were evaluated simultaneously in separate experiments. MICs of SMT19969 were determined against 30 genotypically distinct C. difficile ribotypes. RESULTS: SMT19969 was 7- and 17-fold more active against C. difficile than metronidazole and vancomycin, respectively, against a panel of genotypically distinct isolates (P < 0.05). Both SMT19969 dosing regimens demonstrated little antimicrobial activity against indigenous gut microflora groups except clostridia. SMT19969 inhibited C. difficile growth and repressed C. difficile cytotoxin titres in the gut model. CONCLUSIONS: These data suggest that SMT19969 is a narrow-spectrum and potent antimicrobial agent against C. difficile. Additional studies evaluating SMT19969 in other models of C. difficile infection are warranted, with human studies to place these gut model observations in context.Peer reviewedFinal Published versio

An analysis of type F2 software measurement standards for profile surface texture parameters

This paper reports on an in-depth analysis of ISO 5436 part 2 type F2 reference software for the calculation of profile surface texture parameters that has been performed on the input, implementation and output results of the reference software developed by the National Physical Laboratory (NPL), the National Institute of Standards and Technology (NIST) and Physikalisch-Technische Bundesanstalt (PTB). Surface texture parameters have been calculated for a selection of 17 test data files obtained from the type F1 reference data sets on offer from NPL and NIST. The surface texture parameter calculation results show some disagreements between the software methods of the National Metrology Institutes. These disagreements have been investigated further, and some potential explanations are given