Evolutionary Divergence in Developmental Strategies and Neuromodulatory Control Systems of Two Amphibian Locomotor Networks

Attempts to understand the neural mechanisms which produce behaviour must consider both prevailing sensory cues and the central cellular and synaptic changes they direct. At each level, neuromodulation can additionally shape the final output. We have investigated neuromodulation in the developing spinal motor networks in hatchling tadpoles of two closely related amphibians, Xenopus laevis and Rana temporaria to examine the subtle differences in their behaviours that could be attributed to their evolutionary divergence. At the point of hatching, both species can swim in response to a mechanosensory stimulus, however Rana embryos often display a more forceful, non-locomotory coiling behaviour. Whilst the synaptic drive that underlies these behaviours appears similar, subtle inter-specific differences in neuronal properties shape motor outputs in different ways. For example, Rana neurons express N-methyl-D-aspartate (NMDA)/serotonin (5-HT)-dependent oscillations, not present in hatchling Xenopus and many also exhibit a prominent slow spike after-hyperpolarisation. Such properties may endow the spinal circuitry of Rana with the ability to produce a more flexible range of outputs. Finally, we compare the roles of the neuromodulators 5-HT, noradrenaline (NA) and nitric oxide (NO) in shaping motor outputs. 5-HT increases burst durations during swimming in both Xenopus and Rana, but 5-HT dramatically slows the cycle period in Rana with little effect in Xenopus. Three distinct, but presumably homologous NO-containing brainstem clusters of neurons have been described, yet the effects of NO differ between species. In Xenopus, NO slows and shortens swimming in a manner similar to NA, yet in Rana NO and NA elicit the non-rhythmic coiling pattern

Integrating Research Data Management into Geographical Information Systems

Ocean modelling requires the production of high-fidelity computational meshes upon which to solve the equations of motion. The production of such meshes by hand is often infeasible, considering the complexity of the bathymetry and coastlines. The use of Geographical Information Systems (GIS) is therefore a key component to discretising the region of interest and producing a mesh appropriate to resolve the dynamics. However, all data associated with the production of a mesh must be provided in order to contribute to the overall recomputability of the subsequent simulation. This work presents the integration of research data management in QMesh, a tool for generating meshes using GIS. The tool uses the PyRDM library to provide a quick and easy way for scientists to publish meshes, and all data required to regenerate them, to persistent online repositories. These repositories are assigned unique identifiers to enable proper citation of the meshes in journal articles.Comment: Accepted, camera-ready version. To appear in the Proceedings of the 5th International Workshop on Semantic Digital Archives (http://sda2015.dke-research.de/), held in Pozna\'n, Poland on 18 September 2015 as part of the 19th International Conference on Theory and Practice of Digital Libraries (http://tpdl2015.info/

Improving the worthiness of the Elder problem as a benchmark for buoyancy driven convection models

An important trapping mechanism associated with the geosequestration of CO~2~ is that of dissolution into the formation water. Although supercritical CO~2~ is significantly less dense than water, experimental data reported in the literature show that the density of an aqueous solution of CO~2~ could be slightly greater. Under normal situations, the transfer of gas to solution is largely controlled by the relatively slow process of molecular diffusion. However, the presence of variable densities can trigger off gravity instabilities leading to much larger-scale convection processes. Such processes can potentially enhance rates of dissolution by an order of magnitude. Consequently there is a need for future performance assessment models to incorporate buoyancy driven convection (BDC). A major issue associated with BDC models is that of grid convergence when benchmarking to the Elder problem. The Elder problem originates from a heat convection experiment whereby a rectangular Hele-Shaw cell was heated over the central half of its base. A quarter of the way through the experiment, Elder (1967) observed six plumes, with four narrow plumes in the center and two larger plumes at the edges. As the experiment progressed, only four plumes remained. The issue is that depending on the grid resolution used when seeking to model this problem, modelers have found that different schemes yield steady states with either one, two or three plumes. The aim of this paper is to clarify and circumvent the issue of multiple steady state solutions in the Elder problem using a pseudospectral method

Mean eigenvalues for simple, simply connected, compact Lie groups

We determine for each of the simple, simply connected, compact and complex Lie groups SU(n), Spin$(4n+2)$ and $E_6$ that particular region inside the unit disk in the complex plane which is filled by their mean eigenvalues. We give analytical parameterizations for the boundary curves of these so-called trace figures. The area enclosed by a trace figure turns out to be a rational multiple of $\pi$ in each case. We calculate also the length of the boundary curve and determine the radius of the largest circle that is contained in a trace figure. The discrete center of the corresponding compact complex Lie group shows up prominently in the form of cusp points of the trace figure placed symmetrically on the unit circle. For the exceptional Lie groups $G_2$, $F_4$ and $E_8$ with trivial center we determine the (negative) lower bound on their mean eigenvalues lying within the real interval $[-1,1]$. We find the rational boundary values -2/7, -3/13 and -1/31 for $G_2$, $F_4$ and $E_8$, respectively.Comment: 12 pages, 8 figure

Irrigation-Yield Production Functions and Irrigation Water Use Effciency Response of Drought-Tolerant and Non-Drought-Tolerant Maize Hybrids under Different Irrigation Levels, Population Densities, and Environments

Irrigation-yield production functions (IYPFs), irrigation water use effciency (IWUE), and grain production per unit of applied irrigation of non-drought-tolerant (NDT) and drought-tolerant (DT) maize (Zea mays L.) hybrids were quantified in four locations with different climates in Nebraska [Concord (sub-humid), Clay Center (transition zone between sub-humid and semi-arid); North Platte (semi-arid); and, Scottsbluff (semi-arid)] during three growing seasons (2010, 2011, and 2012) at three irrigation levels (fully-irrigated treatment (FIT), early cut-off (ECOT), and rainfed (RFT)) under two plant population densities (PPDs) (low-PPD; 59,300 plants ha-1; and, high-PPD, 84,000 plants ha-1). Overall, DT hybrids’ performance was superior to NDT hybrid at RFT, ECT, and FIT conditions, as confirmed by the yield response, IYPF and IWUE when all locations, years, and PPDs were averaged. The yield response to water was greater with the high-PPD than the low-PPD in most cases. The magnitude of the highest yields for DT hybrids ranged from 7.3 (low-PPD) to 8.5% (high-PPD) under RFT, 3.7 (low-PPD) to 9.6% (high-PPD) under ECOT, and 3.9% (high-PPD) under FIT higher than NDT hybrid. Relatively, DT hybrids can resist drought-stress conditions longer than NDT hybrid with fewer penalties in yield reduction and maintain comparable or even higher yield production at non-stress-water conditions

Recent developments in the application of risk analysis to waste technologies.

The European waste sector is undergoing a period of unprecedented change driven by business consolidation, new legislation and heightened public and government scrutiny. One feature is the transition of the sector towards a process industry with increased pre-treatment of wastes prior to the disposal of residues and the co-location of technologies at single sites, often also for resource recovery and residuals management. Waste technologies such as in-vessel composting, the thermal treatment of clinical waste, the stabilisation of hazardous wastes, biomass gasification, sludge combustion and the use of wastes as fuel, present operators and regulators with new challenges as to their safe and environmentally responsible operation. A second feature of recent change is an increased regulatory emphasis on public and ecosystem health and the need for assessments of risk to and from waste installations. Public confidence in waste management, secured in part through enforcement of the planning and permitting regimes and sound operational performance, is central to establishing the infrastructure of new waste technologies. Well-informed risk management plays a critical role. We discuss recent developments in risk analysis within the sector and the future needs of risk analysis that are required to respond to the new waste and resource management agenda

A commentary on recent water safety initiatives in the context of water utility risk management.

Over the last decade, suppliers of drinking water have recognised the limitations of relying solely on end-product monitoring to ensure safe water quality and have sought to reinforce their approach by adopting preventative strategies where risks are proactively identified, assessed and managed. This is leading to the development of water safety plans; structured ‘route maps’ for managing risks to water supply, from catchment to consumer taps. This paper reviews the Hazard Analysis and Critical Control Point (HACCP) procedure on which many water safety plans are based and considers its appropriateness in the context of drinking water risk management. We examine water safety plans in a broad context, looking at a variety of monitoring, optimisation and risk management initiatives that can be taken to improve drinking water safety. These are cross-compared using a simple framework that facilitates an integrated approach to water safety. Finally, we look at how risk management practices are being integrated across water companies and how this is likely to affect the future development of water safety p

Translating research into policy and practice in developing countries: a case study of magnesium sulphate for pre-eclampsia.

BACKGROUND: The evidence base for improving reproductive health continues to grow. However, concerns remain that the translation of this evidence into appropriate policies is partial and slow. Little is known about the factors affecting the use of evidence by policy makers and clinicians, particularly in developing countries. The objective of this study was to examine the factors that might affect the translation of randomised controlled trial (RCT) findings into policies and practice in developing countries. METHODS: The recent publication of an important RCT on the use of magnesium sulphate to treat pre-eclampsia provided an opportunity to explore how research findings might be translated into policy. A range of research methods, including a survey, group interview and observations with RCT collaborators and a survey of WHO drug information officers, regulatory officials and obstetricians in 12 countries, were undertaken to identify barriers and facilitators to knowledge translation. RESULTS: It proved difficult to obtain reliable data regarding the availability and use of commonly used drugs in many countries. The perceived barriers to implementing RCT findings regarding the use of magnesium sulphate for pre-eclampsia include drug licensing and availability; inadequate and poorly implemented clinical guidelines; and lack of political support for policy change. However, there were significant regional and national differences in the importance of specific barriers. CONCLUSION: The policy changes needed to ensure widespread availability and use of magnesium sulphate are variable and complex. Difficulties in obtaining information on availability and use are combined with the wide range of barriers across settings, including a lack of support from policy makers. This makes it difficult to envisage any single intervention strategy that might be used to promote the uptake of research findings on magnesium sulphate into policy across the study settings. The publication of important trials may therefore not have the impacts on health care that researchers hope for

The future of sustainable cities: governance, policy and knowledge

The aim of this special issue is to address a conceptual and empirical gap in the existing literature on sustainable cities. Uniquely, it brings together questions about the “what” (in this case, the content and representation of urban policy) of research on urban sustainability with “how” (the social organisation of knowledge and action) through the results of collaborative and comparative work. The special issue contains curated contributions that draw upon the findings of a comparative international project funded by Mistra (Swedish Foundation for Strategic Environmental Research) through the Mistra Urban Futures Centre, based in Gothenburg. The project sought to bring together two questions that are usually treated as separate in existing research approaches. They are the “what” and “how” of sustainable cities’ debates. By working in partnership with local policy-makers, practitioners and universities, each local area entered into a process of collaborative design in order to examine assumptions, expectations, processes and the outcomes of knowledge co-production. This paper introduces the conceptual ideas behind this initiative and so provides a frame for the reader to situate the contributions. It then outlines those articles to draw connections between them and concludes with a short summary of what research and societal lessons can be learnt from the project